{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T17:57:17Z","timestamp":1777658237969,"version":"3.51.4"},"reference-count":28,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T00:00:00Z","timestamp":1644624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["scholarship 2021. 09461.BD  EcoARUn: POCI-01-0145-FEDER-030310 UIDP\/50017\/2020+UIDB\/50017\/202 UIDB\/04750\/2020  LA\/P\/0064\/2020"],"award-info":[{"award-number":["scholarship 2021. 09461.BD  EcoARUn: POCI-01-0145-FEDER-030310 UIDP\/50017\/2020+UIDB\/50017\/202 UIDB\/04750\/2020  LA\/P\/0064\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Porcine circovirus type 2 (PCV-2) is associated with several syndromes affecting swine, also known as porcine-circovirus-associated diseases, of which post-weaning multi-systemic wasting syndrome stands out due to its high economic impact on swine production. Recent data suggest the increasing circulation of the PCV-2d genotype in several countries worldwide. To provide updated data on PCV-2 genotypes currently circulating in swine in Portugal, we screened wild boar stools collected from several districts across Portugal, during the 2018\u20132020 hunting seasons, for PCV-2 and genetically characterized detected strains. From a total of 76 stool samples of wild boar tested by PCR for the partial PCV-2 ORF2 gene, two sequences were obtained (2\/76; 2.6%, 95% confidence interval: 0.032\u20139.18). Bidirectional sequencing showed that the sequences were 100% identical and both of the PCV-2d genotype, showing for the first time the presence of this genotype in Portugal. Monitoring wild PCV-2 reservoirs is important for both veterinary public health and economic reasons, since PCV-2 infection has a strong economic impact on the swine industry.<\/jats:p>","DOI":"10.3390\/ani12040451","type":"journal-article","created":{"date-parts":[[2022,2,13]],"date-time":"2022-02-13T20:32:30Z","timestamp":1644784350000},"page":"451","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Epidemiology of Porcine Circovirus Type 2 Circulating in Wild Boars of Portugal during the 2018\u20132020 Hunting Seasons Suggests the Emergence of Genotype 2d"],"prefix":"10.3390","volume":"12","author":[{"given":"Al\u00edcia","family":"de Sousa Moreira","sequence":"first","affiliation":[{"name":"ICBAS\u2014School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9742-5643","authenticated-orcid":false,"given":"S\u00e9rgio","family":"Santos-Silva","sequence":"additional","affiliation":[{"name":"ICBAS\u2014School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"}]},{"given":"Jo\u00e3o","family":"Mega","sequence":"additional","affiliation":[{"name":"ICBAS\u2014School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3729-9942","authenticated-orcid":false,"given":"Josman D.","family":"Palmeira","sequence":"additional","affiliation":[{"name":"Department of Biology & CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4570-459X","authenticated-orcid":false,"given":"Rita T.","family":"Torres","sequence":"additional","affiliation":[{"name":"Department of Biology & CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8769-8103","authenticated-orcid":false,"given":"Jo\u00e3o R.","family":"Mesquita","sequence":"additional","affiliation":[{"name":"ICBAS\u2014School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"},{"name":"Epidemiology Research Unit (EPIUnit), Instituto de Sa\u00fade P\u00fablica da Universidade do Porto, 4050-600 Porto, Portugal"},{"name":"Laborat\u00f3rio Para a Investiga\u00e7\u00e3o Integrativa e Translacional em Sa\u00fade Populacional (ITR), 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.meegid.2019.03.013","article-title":"Phylogenetic analysis of porcine circovirus type 2 in Sardinia, Italy, shows genotype 2d circulation among domestic pigs and wild boars","volume":"71","author":"Bonelli","year":"2019","journal-title":"Infect. 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