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Our objective was to study the effect of a novel mitochondrial-directed antioxidant, AntiOxBEN2, on bovine sperm function. This antioxidant was added to the semen capacitation medium (CAP), during the swim-up process, and to the fertilization medium (FERT) during the co-incubation of matured oocytes and capacitated spermatozoa, in concentrations of 0 (control), 1, and 10 \u00b5M. After the swim-up, sperm motility (CASA and visual analysis), vitality (eosin-nigrosin), mitochondrial membrane potential (JC1), intracellular ROS, adenosine triphosphate (ATP) levels, and basal metabolism (Seahorse Xfe96) were evaluated. Embryo development and quality were also assessed. Higher cleavage rates were obtained when 1 \u00b5M AntiOxBEN2 were added to CAP and FERT media (compared to control, p &lt; 0.04). A positive effect of AntiOxBEN2 on intracellular ROS reduction (p = 0.01), on the increment of mitochondrial membrane potential (p \u2264 0.003) and, consequently, on the sperm quality was identified. However, the highest dose impaired progressive motility, ATP production, and the number of produced embryos. The results demonstrate a beneficial effect of AntiOxBEN2 (1 \u00b5M) on sperm capacitation and fertilization processes, thus improving embryonic development. This may constitute a putative novel therapeutic strategy to improve the outcomes of assisted reproductive techniques (ART).<\/jats:p>","DOI":"10.3390\/ani12070804","type":"journal-article","created":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T14:55:35Z","timestamp":1647960935000},"page":"804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of a Novel Hydroxybenzoic Acid Based Mitochondria Directed Antioxidant Molecule on Bovine Sperm Function and Embryo Production"],"prefix":"10.3390","volume":"12","author":[{"given":"Jo\u00e3o Campos","family":"Santos","sequence":"first","affiliation":[{"name":"Biotechnology and Genetic Resources Unit, INIAV\u2014National Institute of Agrarian and Veterinarian Research, Quinta da Fonte Boa, 2005-048 Vale de Santar\u00e9m, Portugal"},{"name":"CIVG, Center for Investigation Vasco da Gama (CIVG), Department of Veterinary Sciences, Escola Universit\u00e1ria Vasco da Gama, 3020-210 Coimbra, Portugal"}]},{"given":"Carla Cruz","family":"Marques","sequence":"additional","affiliation":[{"name":"Biotechnology and Genetic Resources Unit, INIAV\u2014National Institute of Agrarian and Veterinarian Research, Quinta da Fonte Boa, 2005-048 Vale de Santar\u00e9m, Portugal"}]},{"given":"Maria Concei\u00e7\u00e3o","family":"Baptista","sequence":"additional","affiliation":[{"name":"Biotechnology and Genetic Resources Unit, INIAV\u2014National Institute of Agrarian and Veterinarian Research, Quinta da Fonte Boa, 2005-048 Vale de Santar\u00e9m, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3796-0238","authenticated-orcid":false,"given":"Jorge","family":"Pimenta","sequence":"additional","affiliation":[{"name":"Biotechnology and Genetic Resources Unit, INIAV\u2014National Institute of Agrarian and Veterinarian Research, Quinta da Fonte Boa, 2005-048 Vale de Santar\u00e9m, Portugal"},{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, University of Lisboa, Av. da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0834-5698","authenticated-orcid":false,"given":"Jos\u00e9","family":"Teixeira","sequence":"additional","affiliation":[{"name":"CNC-Center for Neuroscience and Cell Biology, CIBB\u2014Centre for Innovative Biomedicine and Biotechnology, IIIUC\u2014Institute for Interdisciplinary Research, University of Coimbra, 3004-504 Coimbra, Portugal"},{"name":"MitoTAG, Biocant Park\u2014Parque Tecnol\u00f3gico de Cantanhede, N\u00facleo 04, Lote 04, 3060-197 Cantanhede, Portugal"}]},{"given":"Liliana","family":"Montezinho","sequence":"additional","affiliation":[{"name":"CIVG, Center for Investigation Vasco da Gama (CIVG), Department of Veterinary Sciences, Escola Universit\u00e1ria Vasco da Gama, 3020-210 Coimbra, Portugal"}]},{"given":"Fernando","family":"Cagide","sequence":"additional","affiliation":[{"name":"CIQUP\/Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1050-2402","authenticated-orcid":false,"given":"Fernanda","family":"Borges","sequence":"additional","affiliation":[{"name":"CIQUP\/Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5201-9948","authenticated-orcid":false,"given":"Paulo J.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CNC-Center for Neuroscience and Cell Biology, CIBB\u2014Centre for Innovative Biomedicine and Biotechnology, IIIUC\u2014Institute for Interdisciplinary Research, University of Coimbra, 3004-504 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7514-6130","authenticated-orcid":false,"given":"Rosa M. 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