{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T08:49:17Z","timestamp":1760345357967,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"National Funds by the Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["2021.04520.BD","UIDB\/CVT\/00772\/2020","UIDB\/04033\/2020"],"award-info":[{"award-number":["2021.04520.BD","UIDB\/CVT\/00772\/2020","UIDB\/04033\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Wild boars are wild ungulates with a wide distribution in Europe, with a relevant role in wildlife and public health. In Spain, high (and sometimes artificial) densities of wild boars are responsible for several health problems. Regular surveys, with hunters\u2019 collaboration, are crucial to monitor these diseases. Histological analyses were performed for lung, liver, and kidneys from 72 wild boars (58 from Zamora, 16 from Palencia). Lungs were the most affected organs, mainly revealing parasitic pneumonia (34.7%). Hydropic, vacuolar, and other cellular changes (33.3%) and congestion (16.7%) were found in the liver, and only 30.6% of the wild boars presented no alterations in this organ. Regarding the kidney, non-purulent nephritis (22.2%) was the most common lesion. This study gives an overview of the health status of wild boar populations in Castile and Le\u00f3n. Other laboratory analyses are needed to obtain definitive diagnoses of these lesions, reach other conclusions, or apply any mitigation strategies to protect animals\u2019 or consumers\u2019 health.<\/jats:p>","DOI":"10.3390\/ani12233282","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T03:00:13Z","timestamp":1669345213000},"page":"3282","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Wild Boars (Sus scrofa, L. 1758) from Castile and Leon Region (Spain): A Histopathology Survey"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4841-0143","authenticated-orcid":false,"given":"Catarina","family":"Jota Baptista","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences (ECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"},{"name":"Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal"},{"name":"Institute of Biomedicine (IBIOMED), University of Le\u00f3n, 24071 Le\u00f3n, Spain"}]},{"given":"Jos\u00e9 M.","family":"Gonzalo-Orden","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine (IBIOMED), University of Le\u00f3n, 24071 Le\u00f3n, Spain"}]},{"given":"Lu\u00eds J.","family":"Merino-Goyenechea","sequence":"additional","affiliation":[{"name":"Departament of Biomedical Sciences, University of Le\u00f3n, 24071 Le\u00f3n, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9519-4044","authenticated-orcid":false,"given":"Paula A.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences (ECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"},{"name":"Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1912-1457","authenticated-orcid":false,"given":"Fernanda","family":"Seixas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences (ECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal"},{"name":"Veterinary and Animal Research Center (CECAV), AL4Animals, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1089\/fpd.2018.2512","article-title":"Wild Boar: A Reservoir of Foodborne Zoonoses","volume":"16","year":"2019","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1038\/nature06536","article-title":"Global Trends in Emerging Infectious Diseases","volume":"451","author":"Jones","year":"2008","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Mu\u00f1oz-Mendoza, M., Marreros, N., Boadella, M., Gort\u00e1zar, C., Men\u00e9ndez, S., de Juan, L., Bezos, J., Romero, B., Copano, M.F., and Amado, J. (2013). Wild Boar Tuberculosis in Iberian Atlantic Spain: A Different Picture from Mediterranean Habitats. BMC Vet. Res., 9.","DOI":"10.1186\/1746-6148-9-176"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.1098\/rstb.2009.0086","article-title":"Wild Boars as Sources for Infectious Diseases in Livestock and Humans","volume":"364","author":"Meng","year":"2009","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1111\/tbed.12820","article-title":"Zoonotic Pathogens from Feral Swine That Pose a Significant Threat to Public Health","volume":"65","author":"Brown","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.prevetmed.2017.08.009","article-title":"Animal Tuberculosis Maintenance at Low Abundance of Suitable Wildlife Reservoir Hosts: A Case Study in Northern Spain","volume":"146","author":"Acevedo","year":"2017","journal-title":"Prev. Vet. Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Gavier-Wid\u00e9n, D., Chambers, M., Gort\u00e1zar, C., Delahay, R., Cromie, R., and Lind\u00e9n, A. (2012). Mycobacteria Infections. Infectious Diseases of Wild Mammals and Birds in Europe, Wiley-Blackwell.","DOI":"10.1002\/9781118342442"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"818","DOI":"10.3390\/applmicrobiol2040062","article-title":"Detection of Hepatitis E Virus (HEV) in Pigs and in the Wild Boar (Sus scrofa) Population of Chieti Province, Abruzzo Region, Italy","volume":"2","author":"Aprea","year":"2022","journal-title":"Appl. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1089\/vbz.2020.2756","article-title":"Seroprevalence of Hepatitis e Virus Infection among Wild Boars in Western Bulgaria","volume":"21","author":"Tsachev","year":"2021","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"128848","DOI":"10.1016\/j.chemosphere.2020.128848","article-title":"Wild Boar (Sus scrofa) as Bioindicator for Environmental Exposure to Organic Pollutants","volume":"268","year":"2021","journal-title":"Chemosphere"},{"key":"ref_11","unstructured":"Fernando Portela, J. (2014). La Industria del Vino y La Viticultura en Castilla y Le\u00f3n: Su Incidencia en el Paisaje y en el Desarrollo Rural, Universidad de Valladolid."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1111\/tbed.12167","article-title":"Temporal Trend of Tuberculosis in Wild Ungulates from Mediterranean Spain","volume":"60","author":"Vicente","year":"2013","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_13","first-page":"3154","article-title":"Outbreak of Trichinellosis in Spain and Sweden Due to Consumption of Wild Boar Meat Contaminated with Trichinella Britovi","volume":"12","author":"Gallardo","year":"2007","journal-title":"Wkly. Releases (1997\u20132007)"},{"key":"ref_14","unstructured":"de Guadalupe Carri\u00e7o Neves, M. (2013). Contribui\u00e7\u00e3o Para a Caracteriza\u00e7\u00e3o Do Parasitismo em Su\u00ednos de Ra\u00e7a Ib\u00e9rica e Javalis Silvestres das Comunidades Aut\u00f3nomas da Extremadura e Castilla y Le\u00f3n (Espanha) e dos Factores de Risco Associados, Universidade T\u00e9cnica de Lisboa-Faculdade de Medicina Veterin\u00e1ria."},{"key":"ref_15","first-page":"197","article-title":"Health Risks in Game Production: The Wild Boar","volume":"16","author":"Vincente","year":"2004","journal-title":"Galemys"},{"key":"ref_16","first-page":"475","article-title":"The Role of Histopathology as a Complementary Diagnostic Tool in the Monitoring of Bovine Tuberculosis","volume":"9","author":"Maldonado","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1093\/ilar\/ilv043","article-title":"Wildlife Pathology Studies and How They Can Inform Public Health","volume":"56","author":"McNamara","year":"2016","journal-title":"ILAR J."},{"key":"ref_18","unstructured":"World Organisation for Animal Health (OiE) (2015). Guidelines for Wildlife Disease Surveillance: An Overview, World Organisation for Animal Health (OiE)."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.7589\/0090-3558-45.4.1048","article-title":"Epidemiology of Mycobacterium Bovis Infection in Wild Boar (Sus scrofa) from Portugal","volume":"45","author":"Santos","year":"2009","journal-title":"J. Wildl. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1097\/00000478-200004000-00015","article-title":"Nodular Lymphoid Hyperplasia of the Lung: A Clinicopathologic Study of 14 Cases","volume":"24","author":"Abbondanzo","year":"2000","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"191","DOI":"10.2478\/jvetres-2019-0037","article-title":"Pathomorphological Studies on Wild Boars Infected with Metastrongylus Spp., Ascarops Strongylina, and Macracanthorhynchus Hirudinaceus","volume":"63","author":"Todorova","year":"2019","journal-title":"J. Vet. Res."},{"key":"ref_22","unstructured":"Terio, K.A., McAloose, D., and St. Leger, J. (2018). Suidae and Tayassuidae. Pathology of Wildlife and Zoo Animals, Elsevier academic Press."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Biondo, N., Takeuti, K.L., Montes, J.H., de Almeida, L.L., de Andrade, C.P., Zlotowski, P., Driemeier, D., and de Barcellos, D.E.S.N. (2021). Bacterial Pneumonia in Captive Wild Boars in Southern Brazil-Etiological and Pathological Causes. Acta Sci. Vet., 49.","DOI":"10.22456\/1679-9216.118917"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/S0002-9610(65)80061-7","article-title":"Pulmonary Contusion","volume":"109","author":"DeMuth","year":"1965","journal-title":"Am. J. Surg."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Sehl, J., Pikalo, J., Sch\u00e4fer, A., Franzke, K., Pannhorst, K., Elnagar, A., Blohm, U., Blome, S., and Breithaupt, A. (2020). Comparative Pathology of Domestic Pigs and Wild Boar Infected with the Moderately Virulent African Swine Fever Virus Strain \u201cEstonia 2014\u201d. Pathogens, 9.","DOI":"10.20944\/preprints202007.0747.v1"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Garc\u00eda, A., Hurtado-Carneiro, V., Herrero-De-Dios, C., Dongil, P., Garc\u00eda-Mauri\u00f1o, J.E., S\u00e1nchez, M.D., Sanz, C., and \u00c1lvarez, E. (2021). Storage and Utilization of Glycogen by Mouse Liver during Adaptation to Nutritional Changes Are Glp-1 and Pask Dependent. Nutrients, 13.","DOI":"10.3390\/nu13082552"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"630","DOI":"10.3389\/fmars.2020.00630","article-title":"Anthropogenic Contaminants and Histopathological Findings in Stranded Cetaceans in the Southeastern United States, 2012\u20132018","volume":"7","author":"Lo","year":"2020","journal-title":"Front. Mar. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Gupta, P.K. (2014). Herbicides and Fungicides. Biomarkers in Toxicology, Academic Press.","DOI":"10.1016\/B978-0-12-404630-6.00024-5"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S0021-9673(99)01188-7","article-title":"Evaluation of Surface- and Ground-Water Pollution Due to Herbicides in Agricultural Areas of Zamora and Salamanca (Spain)","volume":"869","year":"2000","journal-title":"J. Chromatogr. A"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1645\/19-196","article-title":"A Case-Study of the Molecular Diagnosis of Echinococcus Multilocularis in Wild Boar with Comments on Its Public Health Significance in Turkey","volume":"106","author":"Kesik","year":"2020","journal-title":"J. Parasitol."},{"key":"ref_31","first-page":"149","article-title":"Molecular Evaluation of a Case of Fasciola Hepatica in Wild Boar in Southwestern Iran: A Case Report","volume":"13","author":"Sarkari","year":"2018","journal-title":"Iran J. Parasitol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Williams, E.S., and Barker, I.K. (2001). Infectious Diseases of Wild Mammals, Iowa State University Press. [3rd ed.].","DOI":"10.1002\/9780470344880"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"786195","DOI":"10.1155\/2015\/786195","article-title":"Fascioliasis: An Ongoing Zoonotic Trematode Infection","volume":"2015","author":"Nyindo","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1964","DOI":"10.3201\/eid1710.101826","article-title":"Zoonotic Ascariasis, United Kingdom","volume":"17","author":"Bendall","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1645\/GE-2828.1","article-title":"Human Infections with Dicrocoelium Dendriticum in Kyrgyzstan: The Tip of the Iceberg?","volume":"97","author":"Jeandron","year":"2011","journal-title":"J. Parasitol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Vieira-Pinto, M., Morais, L., Caleja, C., Themudo, P., Aranha, J., Torres, C., Igrejas, G., Poeta, P., and Martins, C. (2011). Salmonella Spp. in Wild Boar (Sus scrofa): A Public and Animal Health Concern. Game Meat Hygiene in Focus, Wageningen Academic Publishers.","DOI":"10.3920\/978-90-8686-723-3_10"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"63","DOI":"10.14309\/crj.2015.102","article-title":"Eosinophilic Liver Infiltration","volume":"3","author":"Rivera","year":"2015","journal-title":"ACG Case Rep. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1136\/vr.102064","article-title":"Origin of Porcine Circovirus Type 2 (PCV2) from Swine Affected by PCV2-Associated Diseases in Croatia","volume":"174","author":"Novosel","year":"2014","journal-title":"Vet. Rec."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1079\/AHR2005106","article-title":"Porcine Circovirus Diseases","volume":"6","author":"Allan","year":"2005","journal-title":"Anim. Health Res. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"739","DOI":"10.3201\/eid1305.061302","article-title":"Leptospirosis in Urban Wild Boars, Berlin, Germany","volume":"13","author":"Jansen","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1017\/S0950268814003331","article-title":"Prevalence of Leptospira Antibodies in Wild Boars (Sus scrofa) from Northern Portugal: Risk Factor Analysis","volume":"143","author":"Cabral","year":"2015","journal-title":"Epidemiol. Infect."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1017\/S0950268807000052","article-title":"Changes in Epidemiology of Leptospirosis in 2003\u20132004, a Two El Ni\u00f1o Southern Oscillation Period, Guadeloupe Archipelago, French West Indies","volume":"136","author":"Storck","year":"2008","journal-title":"Epidemiol. Infect."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/0021-9975(70)90036-8","article-title":"Leptospirosis in Wild Animals","volume":"80","author":"Michna","year":"1970","journal-title":"J. Comp. Pathol."},{"key":"ref_44","unstructured":"Zachary, J. (2017). Bone Marrow, Blood Cells, and the Lymphoid\/Lymphatic System. Pathologic Basis of Veterinary Disease Expert Consult, Mosby."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/23\/3282\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:26:25Z","timestamp":1760145985000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/12\/23\/3282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,25]]},"references-count":44,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["ani12233282"],"URL":"https:\/\/doi.org\/10.3390\/ani12233282","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2022,11,25]]}}}