{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:43:26Z","timestamp":1765547006563,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para Ci\u00eancia e Tecnologia (FCT)","doi-asserted-by":"publisher","award":["2021.09380.BD"],"award-info":[{"award-number":["2021.09380.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Coronaviruses (CoVs) are part of the Coronaviridae family, and the genera Gamma (\u03b3) and Delta (\u03b4) are found mostly in birds. Migratory birds have an enormous potential for dispersing pathogenic microorganisms. Ducks (order Anseriformes) can host CoVs from birds, with pathogenic expression and high economic impact. This study aimed to identify and characterize the diversity of CoVs in migratory ducks from Portugal. Duck stool samples were collected using cloacal swabs from 72 individuals (Anas platyrhynchos, Anas acuta, and Anas crecca). Among the 72 samples tested, 24 showed amplicons of the expected size. Twenty-three were characterized as Gammacoronavirus and one as Deltacoronavirus (accession numbers ON368935-ON368954; ON721380-ON721383). The Gammacoronaviruses sequences showed greater similarities to those obtained in ducks (Anas platyrhynchos) from Finland and Poland, Anas crecca duck from the USA, and mute swans from Poland. Birds can occupy many habitats and therefore play diverse ecological roles in various ecosystems, especially given their ability to migrate exceptional distances, facilitating the dispersal of microorganisms with animal and\/or human impact. There are a considerable number of studies that have detected CoVs in ducks, but none in Portugal. The present study assessed the circulation of CoVs in wild ducks from Portugal, being the first description of CoVs for these animals in Portugal.<\/jats:p>","DOI":"10.3390\/ani12233283","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T03:00:13Z","timestamp":1669345213000},"page":"3283","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular Detection and Characterization of Coronaviruses in Migratory Ducks from Portugal Show the Circulation of Gammacoronavirus and Deltacoronavirus"],"prefix":"10.3390","volume":"12","author":[{"given":"Mahima","family":"Hemnani","sequence":"first","affiliation":[{"name":"School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3855-7686","authenticated-orcid":false,"given":"David","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Coimbra College of Agriculture, Polytechnic of Coimbra, 3045-601 Coimbra, Portugal"},{"name":"CEF, Forest Research Centre, Edif\u00edcio Prof. Azevedo Gomes, ISA, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"given":"Nuno","family":"Santos","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, InBIO Laborat\u00f3rio Associado, Universidade do Porto, 4485-661 Vair\u00e3o, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9742-5643","authenticated-orcid":false,"given":"Sergio","family":"Santos-Silva","sequence":"additional","affiliation":[{"name":"School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"}]},{"given":"Maria Ester","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"CEF, Forest Research Centre, Edif\u00edcio Prof. Azevedo Gomes, ISA, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"given":"Pedro","family":"Henriques","sequence":"additional","affiliation":[{"name":"Coimbra College of Agriculture, Polytechnic of Coimbra, 3045-601 Coimbra, Portugal"}]},{"given":"Joana","family":"Ferreira-e-Silva","sequence":"additional","affiliation":[{"name":"School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"}]},{"given":"Hugo","family":"Rebelo","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, InBIO Laborat\u00f3rio Associado, Universidade do Porto, 4485-661 Vair\u00e3o, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-689X","authenticated-orcid":false,"given":"Patricia","family":"Poeta","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, Universidade de Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Veterinary and Animal Research Centre, Universidade de Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4 Animals), Universidade de Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Gertrude","family":"Thompson","sequence":"additional","affiliation":[{"name":"School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, InBIO Laborat\u00f3rio Associado, Universidade do Porto, 4485-661 Vair\u00e3o, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8769-8103","authenticated-orcid":false,"given":"Jo\u00e3o R.","family":"Mesquita","sequence":"additional","affiliation":[{"name":"School of Medicine and Biomedical Sciences, Porto University, 4050-313 Porto, Portugal"},{"name":"Epidemiology Research Unit (EPIUnit), Instituto de Sa\u00fade P\u00fablica da Universidade do Porto, 4050-600 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Doma\u0144ska-Blicharz, K., Mi\u0142ek-Krupa, J., and Piku\u0142a, A. 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