{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T23:44:15Z","timestamp":1775691855008,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T00:00:00Z","timestamp":1678838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"VALORMAR\u2014Valoriza\u00e7\u00e3o Integral dos Recursos Marinhos: Potencial, Inova\u00e7\u00e3o Tecnol\u00f3gica e Novas Aplica\u00e7\u00f5es, with the reference 24517 (10\/SI\/2016\u2014I&amp;DT Empresarial\u2014Programas Mobilizadores) within the R&amp;D Institution CIIMAR, co-financed by the European Union through the ERDF and by the European Social Fund (ESF)","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"VALORMAR\u2014Valoriza\u00e7\u00e3o Integral dos Recursos Marinhos: Potencial, Inova\u00e7\u00e3o Tecnol\u00f3gica e Novas Aplica\u00e7\u00f5es, with the reference 24517 (10\/SI\/2016\u2014I&amp;DT Empresarial\u2014Programas Mobilizadores) within the R&amp;D Institution CIIMAR, co-financed by the European Union through the ERDF and by the European Social Fund (ESF)","award":["(IF\/01506\/2014)"],"award-info":[{"award-number":["(IF\/01506\/2014)"]}]},{"DOI":"10.13039\/501100001871","name":"FCT\u2014Foundation for Science and Technology\u2014within the scope of","doi-asserted-by":"publisher","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"FCT\u2014Foundation for Science and Technology\u2014within the scope of","doi-asserted-by":"publisher","award":["(IF\/01506\/2014)"],"award-info":[{"award-number":["(IF\/01506\/2014)"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FCT investigator contract, subsidized by the European Social Fund, and the MCTES (Portuguese Ministry of Science, Technology, and Higher Education) through the POPH (Human Potential Operational Program)","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"FCT investigator contract, subsidized by the European Social Fund, and the MCTES (Portuguese Ministry of Science, Technology, and Higher Education) through the POPH (Human Potential Operational Program)","award":["(IF\/01506\/2014)"],"award-info":[{"award-number":["(IF\/01506\/2014)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>A fish trial was carried out to evaluate the combined effects of temperature and dietary lipid level on the body composition, growth performance, and freshness profile of the European seabass (Dicentrarchus labrax). Fish were kept for 56 days at 20 \u00b0C and 24 \u00b0C and fed on two diets, with 16% and 20% lipid. At the end of the trial, fish were euthanized at two temperature conditions (0.6 \u00b0C or \u22120.6 \u00b0C) and kept on ice for 10 days at 4 \u00b0C to evaluate their freshness condition. Findings demonstrated that fish reared at 24 \u00b0C presented a lower lipid level and a higher daily growth index than those at 20 \u00b0C. Additionally, sensory analysis (Quality Index Method\u2014QIM) and microbiological analysis revealed that fish reared at 24 \u00b0C showed better freshness conditions than those at 20 \u00b0C. However, the 16S rRNA metabarcoding analyses revealed a higher proliferation of genera associated with fish-spoiling bacteria in the skin microbiome of fish reared at 24 \u00b0C, i.e., Vibrio and Acinetobacter, which was not observed in the skin microbiome of fish reared at 20 \u00b0C. Nevertheless, the dietary lipid level did not have any influence on fish freshness. Therefore, our data suggest that the increase in temperature to 24 \u00b0C is beneficial for the growth and freshness profile (lower QIM and lower CFUs\/cm2) of this particular species. Additionally, the lower euthanasia temperature (\u22120.6 \u00b0C) seems to lead to higher fish freshness than the normal temperature (0.6 \u00b0C).<\/jats:p>","DOI":"10.3390\/ani13061068","type":"journal-article","created":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T02:40:11Z","timestamp":1678934411000},"page":"1068","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Combined Effects of Temperature and Dietary Lipid Level on Body Composition, Growth, and Freshness Profile in European Seabass, Dicentrarchus labrax"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4653-4705","authenticated-orcid":false,"given":"Patr\u00edcia G.","family":"Cardoso","sequence":"first","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Odete","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Thais","family":"Cavalheri","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"V\u00e2nia E.","family":"Amorim","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Weiwei","family":"Cao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9685-3435","authenticated-orcid":false,"given":"Diogo A. M.","family":"Alexandrino","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"Department of Environmental Health, School of Health, Polytechnic of Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida 400, 4200-072 Porto, Portugal"}]},{"given":"Zhongjun","family":"Jia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7181-0540","authenticated-orcid":false,"given":"Maria F.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"ICBAS\u2014School of Medicine and Biomedical Sciences, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9485-7528","authenticated-orcid":false,"given":"Paulo","family":"Vaz-Pires","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"ICBAS\u2014School of Medicine and Biomedical Sciences, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"given":"Rodrigo O. A.","family":"Oz\u00f3rio","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, Terminal de Cruzeiros de Leix\u00f5es, Av. General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,15]]},"reference":[{"key":"ref_1","unstructured":"Pachauri, R.K., and Meyer, L.A. (2014). 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