{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T23:58:59Z","timestamp":1774483139137,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,15]],"date-time":"2023-04-15T00:00:00Z","timestamp":1681516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"National Funds by the Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["2021.04520.BD"],"award-info":[{"award-number":["2021.04520.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National Funds by the Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["UIDB\/CVT\/00772\/2020"],"award-info":[{"award-number":["UIDB\/CVT\/00772\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National Funds by the Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"National Funds by the Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Heavy metal(loid) pollution of ecosystems is a current One Health problem. The liver is one of the most affected organs in cases of acute or chronic exposure to abnormal amounts of these substances, inducing histopathologic lesions. In order to assess the influence of heavy metal(loids), forty-five European hedgehogs (Erinaceus europaeus) were submitted to necropsy, and liver samples were collected for a routine histopathology exam and metal(loid)s determination (As, Cd, Co, Cr, Cu and Pb) by ICP-MS. Age was estimated during the necropsy exam. Biliary hyperplasia was the most frequent lesion observed (16\/45; 35.56%). No statistically significant associations were found between biliary hyperplasia and age or sex. Metal(loid)s\u2019 concentrations were higher in animals with biliary hyperplasia (except for As). There was a statistically significant difference for both Cd and Co. For As, Cd and Co, cubs and juveniles animals showed significantly lower concentrations than elder individuals. Only for Pb were significant differences found between females and males. As described in the literature, exposure to metal(loid)s may be a cause of biliary hyperplasia, although further research (including the use of biochemical methods) is needed to support these results. To the authors\u2019 knowledge, this is the first report of this association in hedgehogs.<\/jats:p>","DOI":"10.3390\/ani13081359","type":"journal-article","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T01:34:15Z","timestamp":1681695255000},"page":"1359","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["High Levels of Heavy Metal(loid)s Related to Biliary Hyperplasia in Hedgehogs (Erinaceus europaeus)"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4841-0143","authenticated-orcid":false,"given":"Catarina","family":"Jota Baptista","sequence":"first","affiliation":[{"name":"Departamento de Ci\u00eancias Veterin\u00e1rias, Escola de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias (ECAV), Universidade de Tr\u00e1s-os-Montes e Alto Douro, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o das Tecnologias Agroambientais e Biol\u00f3gicas (CITAB), UTAD, 5000-801 Vila Real, Portugal"},{"name":"Instituto de Biomedicina (IBIOMED), Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"},{"name":"Faculdade de Medicina Veterin\u00e1ria, Universidade Lus\u00f3fona de Humanidades e Tecnologias, 1749-024 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1912-1457","authenticated-orcid":false,"given":"Fernanda","family":"Seixas","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Veterin\u00e1rias, Escola de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias (ECAV), Universidade de Tr\u00e1s-os-Montes e Alto Douro, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Centro de Ci\u00eancia Animal e Veterin\u00e1ria (CECAV), AL4AnimalS, UTAD, 5000-801 Vila Real, Portugal"}]},{"given":"Jos\u00e9 M.","family":"Gonzalo-Orden","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina (IBIOMED), Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6006-344X","authenticated-orcid":false,"given":"Carla","family":"Patinha","sequence":"additional","affiliation":[{"name":"GeoBioTec, Departamento de Geoci\u00eancias, Universidade de Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Pedro","family":"Pato","sequence":"additional","affiliation":[{"name":"GeoBioTec, Departamento de Geoci\u00eancias, Universidade de Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3019-8471","authenticated-orcid":false,"given":"Eduardo","family":"Ferreira da Silva","sequence":"additional","affiliation":[{"name":"GeoBioTec, Departamento de Geoci\u00eancias, Universidade de Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Mar\u00eda","family":"Casero","sequence":"additional","affiliation":[{"name":"RIAS-ALDEIA\u2014Wildlife Rehabilitation and Research Centre, Parque Natural da Ria Formosa, 8700-194 Olh\u00e3o, Portugal"}]},{"given":"Erica","family":"Brazio","sequence":"additional","affiliation":[{"name":"Centro de Recupera\u00e7\u00e3o dos Animais Silvestres de Lisboa (LxCRAS), Parque Florestal de Monsanto, 1500-068 Lisboa, Portugal"}]},{"given":"Ricardo","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"CERVAS-ALDEIA\u2014Centro de Ecologia, Recupera\u00e7\u00e3o e Vigil\u00e2ncia de Animais Selvagens, 6290-520 Gouveia, Portugal"}]},{"given":"Daniela","family":"Costa","sequence":"additional","affiliation":[{"name":"CERVAS-ALDEIA\u2014Centro de Ecologia, Recupera\u00e7\u00e3o e Vigil\u00e2ncia de Animais Selvagens, 6290-520 Gouveia, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1236-3012","authenticated-orcid":false,"given":"Teresa Letra","family":"Mateus","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancia Animal e Veterin\u00e1ria (CECAV), AL4AnimalS, UTAD, 5000-801 Vila Real, Portugal"},{"name":"CISAS-Centre for Research and Development in Agrifood Systems and Sustainability, Escola Superior Agr\u00e1ria, Instituto Polit\u00e9cnico de Viana do Castelo, 4990-706 Viana do Castelo, Portugal"},{"name":"EpiUnit\u2014Instituto de Sa\u00fade P\u00fablica da Universidade do Porto, Laboratory for Integrative and Translational Research in Population Health, 4050-600 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9519-4044","authenticated-orcid":false,"given":"Paula A.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Veterin\u00e1rias, Escola de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias (ECAV), Universidade de Tr\u00e1s-os-Montes e Alto Douro, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o das Tecnologias Agroambientais e Biol\u00f3gicas (CITAB), UTAD, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tovar-S\u00e1nchez, E., Hern\u00e1ndez-Plata, I., Santoyo Mart\u00ednez, M., Valencia-Cuevas, L., and Galante, P.M. 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