{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:09:12Z","timestamp":1760148552446,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT, Portuguese Foundation for Science and Technology","doi-asserted-by":"publisher","award":["UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/CVT\/00772\/2020","SFRH\/BPD\/1152202\/2016"],"award-info":[{"award-number":["UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/CVT\/00772\/2020","SFRH\/BPD\/1152202\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"post-doctoral grant","doi-asserted-by":"publisher","award":["UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/CVT\/00772\/2020","SFRH\/BPD\/1152202\/2016"],"award-info":[{"award-number":["UIDB\/00276\/2020","LA\/P\/0059\/2020","UIDB\/CVT\/00772\/2020","SFRH\/BPD\/1152202\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Physaloptera spp. are parasitic nematodes that infect the gastrointestinal tracts of many carnivores and omnivores. Although they are distributed worldwide, Physaloptera spp. have not been studied in raptors in Portugal. In this study, we report Physaloptera alata in a booted eagle (Aquila pennata) in Portugal. Adult nematodes were discovered in the gizzard of a young booted eagle, and morphological features were consistent with those of the genus Physaloptera. DNA was extracted and a PCR assay performed to amplify a region of the 18S small subunit of the ribosomal RNA gene and the cytochrome c oxidase subunit I gene. The resulting PCR products were Sanger-sequenced, and comparison with the available sequences in the GenBank database confirmed the initial morphological classification as Physaloptera sp. Phylogenetic analysis clustered the sequence within the Physaloptera group. The presence of this parasite in raptors from Portugal is of particular importance to wildlife rehabilitation centers, disease ecologists, and wildlife professionals. Furthermore, we produced a new genetic sequence and have added it to the GenBank database of parasites in birds of prey.<\/jats:p>","DOI":"10.3390\/ani13101669","type":"journal-article","created":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T06:32:58Z","timestamp":1684391578000},"page":"1669","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Morphological and Molecular Identification of Physaloptera alata (Nematoda: Spirurida) in a Booted Eagle (Aquila pennata) from Portugal"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4083-1488","authenticated-orcid":false,"given":"Zaida","family":"Renter\u00eda-Sol\u00eds","sequence":"first","affiliation":[{"name":"Institute for Parasitology, Centre of Infection Medicine, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 35, 04103 Leipzig, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4096-2581","authenticated-orcid":false,"given":"David W.","family":"Ramilo","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Portugal"},{"name":"Faculty of Veterinary Medicine, Lus\u00f3fona University\u2014Lisbon University Centre, 1749-024 Lisbon, Portugal"}]},{"given":"Ronald","family":"Schm\u00e4schke","sequence":"additional","affiliation":[{"name":"Institute for Parasitology, Centre of Infection Medicine, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 35, 04103 Leipzig, Germany"}]},{"given":"Sandra","family":"Gawlowska","sequence":"additional","affiliation":[{"name":"Institute for Parasitology, Centre of Infection Medicine, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 35, 04103 Leipzig, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1909-4540","authenticated-orcid":false,"given":"Jorge","family":"Correia","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Portugal"}]},{"given":"Filipa","family":"Lopes","sequence":"additional","affiliation":[{"name":"CERAS\u2014Centro de Estudos e Recupera\u00e7\u00e3o de Animais Selvagens, Quercus Associa\u00e7\u00e3o Nacional de Conserva\u00e7\u00e3o da Natureza, 6000-909 Castelo Branco, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7548-667X","authenticated-orcid":false,"given":"Lu\u00eds","family":"Madeira de Carvalho","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6145-7560","authenticated-orcid":false,"given":"Lu\u00eds","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Portugal"},{"name":"Department of Veterinary Sciences, and Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6632-1224","authenticated-orcid":false,"given":"Isabel","family":"Pereira da Fonseca","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"ref_1","first-page":"129","article-title":"Parasitation of falconiforms, strigiform and passeriform (Corvidae) birds in Spain","volume":"53","author":"Gomez","year":"1993","journal-title":"Res. 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