{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T06:16:17Z","timestamp":1769580977674,"version":"3.49.0"},"reference-count":76,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology (FCT)","award":["UIDB\/04308\/2020"],"award-info":[{"award-number":["UIDB\/04308\/2020"]}]},{"name":"Science and Technology (FCT)","award":["UIDP\/04308\/2020"],"award-info":[{"award-number":["UIDP\/04308\/2020"]}]},{"name":"Science and Technology (FCT)","award":["LANZADERA_2024"],"award-info":[{"award-number":["LANZADERA_2024"]}]},{"name":"Science and Technology (FCT)","award":["PIFULPGC-2017-CCSALUD-3"],"award-info":[{"award-number":["PIFULPGC-2017-CCSALUD-3"]}]},{"name":"General Foundation of the University of Salamanca","award":["UIDB\/04308\/2020"],"award-info":[{"award-number":["UIDB\/04308\/2020"]}]},{"name":"General Foundation of the University of Salamanca","award":["UIDP\/04308\/2020"],"award-info":[{"award-number":["UIDP\/04308\/2020"]}]},{"name":"General Foundation of the University of Salamanca","award":["LANZADERA_2024"],"award-info":[{"award-number":["LANZADERA_2024"]}]},{"name":"General Foundation of the University of Salamanca","award":["PIFULPGC-2017-CCSALUD-3"],"award-info":[{"award-number":["PIFULPGC-2017-CCSALUD-3"]}]},{"name":"Universidad de Las Palmas de Gran Canaria","award":["UIDB\/04308\/2020"],"award-info":[{"award-number":["UIDB\/04308\/2020"]}]},{"name":"Universidad de Las Palmas de Gran Canaria","award":["UIDP\/04308\/2020"],"award-info":[{"award-number":["UIDP\/04308\/2020"]}]},{"name":"Universidad de Las Palmas de Gran Canaria","award":["LANZADERA_2024"],"award-info":[{"award-number":["LANZADERA_2024"]}]},{"name":"Universidad de Las Palmas de Gran Canaria","award":["PIFULPGC-2017-CCSALUD-3"],"award-info":[{"award-number":["PIFULPGC-2017-CCSALUD-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Climatic and various socio-geographical variables have significantly influenced the global spread of Dirofilaria immitis. The coastal district of Aveiro, Portugal, marked by its unique hydrographic structure, Ria de Aveiro, and a concerning rise in heartworm disease, was the focus of our study. We aimed to update the prevalence of D. immitis in dogs and the seroprevalence in cats and humans, correlating these data with epidemiological information. A total of 430 dogs were sampled for D. immitis antigens, and 426 cats and 398 humans for D. immitis and Wolbachia sp. antibodies. In addition, we developed and validated an infection risk map for D. immitis with the geolocation of positive samples. Our results indicate a canine prevalence of 4.7%, peaking at 16.7% in Vagos. Feline and human seroprevalences were 8.9% (26.7% in Espinho) and 3.0% (Vagos presented the most alarming results), respectively. Positive samples were found in both high- and low-risk areas highlighting the need for chemoprophylaxis in all municipalities. Risk factors identified included lack of vaccination and internal deworming in cats, while dogs faced risks from inadequate vaccination and outdoor exposure. Our study identifies Aveiro as an endemic area, with a need for control measures to address this public health threat.<\/jats:p>","DOI":"10.3390\/ani15070952","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T10:54:48Z","timestamp":1743159288000},"page":"952","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Raising Awareness of Canine, Feline and Human Dirofilariosis in Aveiro, Portugal: A One Health Perspective"],"prefix":"10.3390","volume":"15","author":[{"given":"Joana","family":"Esteves-Guimar\u00e3es","sequence":"first","affiliation":[{"name":"Cl\u00ednica Veterin\u00e1ria Aanifeira, 4520-409 Santa Maria da Feira, Portugal"},{"name":"Internal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2683-7592","authenticated-orcid":false,"given":"Jos\u00e9 Alberto","family":"Montoya-Alonso","sequence":"additional","affiliation":[{"name":"Internal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, Spain"},{"name":"Zoonotic Infections and One Health Group, Biomedical Research Institute of Salamanca (IBSAL), Centre for Environmental Studies and Rural Dynamization (CEADIR), Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno, 37007 Salamanca, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4273-2413","authenticated-orcid":false,"given":"Jorge Isidoro","family":"Matos","sequence":"additional","affiliation":[{"name":"Internal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, Spain"}]},{"given":"Elmano","family":"Ramalheira","sequence":"additional","affiliation":[{"name":"Clinical Pathology Department, Baixo Vouga-Public Business Entity (EPE) Hospital Centre, Avenida Artur Ravara, 3810-501 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6509-910X","authenticated-orcid":false,"given":"Elena","family":"Carret\u00f3n","sequence":"additional","affiliation":[{"name":"Internal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, Spain"},{"name":"Zoonotic Infections and One Health Group, Biomedical Research Institute of Salamanca (IBSAL), Centre for Environmental Studies and Rural Dynamization (CEADIR), Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno, 37007 Salamanca, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6009-7658","authenticated-orcid":false,"given":"Ivan","family":"Rodr\u00edguez-Escolar","sequence":"additional","affiliation":[{"name":"Zoonotic Infections and One Health Group, Biomedical Research Institute of Salamanca (IBSAL), Centre for Environmental Studies and Rural Dynamization (CEADIR), Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno, 37007 Salamanca, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9070-6709","authenticated-orcid":false,"given":"Alfonso","family":"Balmori-de la Puente","sequence":"additional","affiliation":[{"name":"Zoonotic Infections and One Health Group, Biomedical Research Institute of Salamanca (IBSAL), Centre for Environmental Studies and Rural Dynamization (CEADIR), Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno, 37007 Salamanca, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8302-0449","authenticated-orcid":false,"given":"Manuel","family":"Collado-Cuadrado","sequence":"additional","affiliation":[{"name":"Zoonotic Infections and One Health Group, Biomedical Research Institute of Salamanca (IBSAL), Centre for Environmental Studies and Rural Dynamization (CEADIR), Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno, 37007 Salamanca, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2699-1482","authenticated-orcid":false,"given":"Rodrigo","family":"Morch\u00f3n","sequence":"additional","affiliation":[{"name":"Internal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, Spain"},{"name":"Zoonotic Infections and One Health Group, Biomedical Research Institute of Salamanca (IBSAL), Centre for Environmental Studies and Rural Dynamization (CEADIR), Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno, 37007 Salamanca, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3873-3886","authenticated-orcid":false,"given":"Ana Patr\u00edcia","family":"Fontes-Sousa","sequence":"additional","affiliation":[{"name":"Internal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, Spain"},{"name":"Centre for Pharmacological Research and Drug Innovation (MedInUP\/RISE-Health), Department of Immuno-Physiology and Pharmacology, Veterinary Hospital of the University of Porto (UPVET), School of Medicine and Biomedical Sciences (ICBAS), University of Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/j.cvsm.2009.06.003","article-title":"Heartworm biology, 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