{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T13:00:50Z","timestamp":1763470850312,"version":"3.45.0"},"reference-count":104,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P.","doi-asserted-by":"publisher","award":["UIDB\/04129\/2020"],"award-info":[{"award-number":["UIDB\/04129\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Associate Laboratory TERRA","doi-asserted-by":"publisher","award":["LA\/P\/0092\/2020"],"award-info":[{"award-number":["LA\/P\/0092\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Crude protein (CP) decrease coupled to amino acid (AAs) supplementation is interesting for swine nutrition. A proteomics approach unraveled physiological events underlying differences between sexes fed two diets: control and low CP in fast-growing Duroc X (Landrace X Large white) finishing pigs. Sixty animals per sex were distributed in pens (five replicate pens\/group), fed ad libitum with two isoenergetic diets (145 vs. 125 g CP\/kg with AA supplementation; control and low-CP) for 42 days. Semimembranosus muscle was sampled. Five samples per group were used for Label Free proteomics. Four comparisons were considered: Control Males vs. Control Females; Control Females vs. Low CP females; Control males vs. Low CP males, and Low CP males vs. Low CP females, identifying, respectively, 26, 19, 12, and 11 DAPs (Differentially Accumulated Proteins). Control males had higher abundance of proteins related to cell differentiation and growth compared to females, highlighting continual muscle accretion in the former and lipogenesis onset in the latter. Control females and males had increased DAPs related to tissue growth and differentiation compared to Low CP animals that had increased lipid accretion. Both sexes reacted similarly to the two diets. Low CP diet led to advanced lipogenesis and decreased muscle accretion pathways.<\/jats:p>","DOI":"10.3390\/ani15223325","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T12:33:04Z","timestamp":1763469184000},"page":"3325","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of a Reduced-Protein Diet Supplemented with Essential Amino Acids on the Muscle Proteome of Female and Entire Male Finishing Pigs"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7810-3988","authenticated-orcid":false,"given":"Andr\u00e9 M.","family":"de Almeida","sequence":"first","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6362-8255","authenticated-orcid":false,"given":"Hugo","family":"Os\u00f3rio","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"IPATIMUP\u2014Institute of Molecular Pathology and Immunology of the University of Porto, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Departamento de Patologia, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3005-2675","authenticated-orcid":false,"given":"Mar\u00eda \u00c1ngeles","family":"Latorre","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza-IA2, Calle Miguel Servet 177, 50013 Zaragoza, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6445-849X","authenticated-orcid":false,"given":"Javier","family":"\u00c1lvarez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Escuela Polit\u00e9cnica Superior, Universidad de Zaragoza-IA2, Carretera de Cuarte s\/n, 22071 Huesca, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"de Almeida, A.M., Latorre, M.A., and Alvarez-Rodriguez, J. 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