{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T22:35:24Z","timestamp":1764542124294,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T00:00:00Z","timestamp":1617408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/118018\/2016"],"award-info":[{"award-number":["SFRH\/BD\/118018\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FCT\/MCTES (PIDDAC)","award":["Project UID\/EQU\/00511\/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy \u2013 LEPABE"],"award-info":[{"award-number":["Project UID\/EQU\/00511\/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy \u2013 LEPABE"]}]},{"name":"European Union\u2019s Horizon 2020 research and innovation program","award":["810685"],"award-info":[{"award-number":["810685"]}]},{"name":"Norte Portugal Regional Operational Programme (NORTE 2020), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF)","award":["Project \u201cLEPABE-2-ECO-INNOVATION\u201d \u2013 NORTE-01-0145-FEDER-000005"],"award-info":[{"award-number":["Project \u201cLEPABE-2-ECO-INNOVATION\u201d \u2013 NORTE-01-0145-FEDER-000005"]}]},{"DOI":"10.13039\/100008398","name":"VILLUM FONDEN","doi-asserted-by":"publisher","award":["VKR18333"],"award-info":[{"award-number":["VKR18333"]}],"id":[{"id":"10.13039\/100008398","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The emergence of bacterial resistance to traditional small-molecule antibiotics is fueling the search for innovative strategies to treat infections. Inhibiting the expression of essential bacterial genes using antisense oligonucleotides (ASOs), particularly composed of nucleic acid mimics (NAMs), has emerged as a promising strategy. However, their efficiency depends on their association with vectors that can translocate the bacterial envelope. Vitamin B12 is among the largest molecules known to be taken up by bacteria and has very recently started to gain interest as a trojan-horse vector. Gapmers and steric blockers were evaluated as ASOs against Escherichia coli (E. coli). Both ASOs were successfully conjugated to B12 by copper-free azide-alkyne click-chemistry. The biological effect of the two conjugates was evaluated together with their intracellular localization in E. coli. Although not only B12 but also both B12-ASO conjugates interacted strongly with E. coli, they were mostly colocalized with the outer membrane. Only 6\u20139% were detected in the cytosol, which showed to be insufficient for bacterial growth inhibition. These results suggest that the internalization of B12-ASO conjugates is strongly affected by the low uptake rate of the B12 in E. coli and that further studies are needed before considering this strategy against biofilms in vivo.<\/jats:p>","DOI":"10.3390\/antibiotics10040379","type":"journal-article","created":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T22:03:36Z","timestamp":1617487416000},"page":"379","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Can Vitamin B12 Assist the Internalization of Antisense LNA Oligonucleotides into Bacteria?"],"prefix":"10.3390","volume":"10","author":[{"given":"Sara","family":"Pereira","sequence":"first","affiliation":[{"name":"Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Faculty of Engineering, University of Porto, R. Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Ruwei","family":"Yao","sequence":"additional","affiliation":[{"name":"Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6774-4333","authenticated-orcid":false,"given":"Mariana","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Faculty of Engineering, University of Porto, R. Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3932-5921","authenticated-orcid":false,"given":"Per Trolle","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9835-1009","authenticated-orcid":false,"given":"Jesper","family":"Wengel","sequence":"additional","affiliation":[{"name":"Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark"}]},{"given":"Nuno Filipe","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Faculty of Engineering, University of Porto, R. Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"Rita","family":"Sobral Santos","sequence":"additional","affiliation":[{"name":"Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Faculty of Engineering, University of Porto, R. Dr. Roberto Frias, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1126\/science.aaa2868","article-title":"A return to the pre-antimicrobial era?","volume":"347","author":"Baker","year":"2015","journal-title":"Science"},{"key":"ref_2","unstructured":"WHO (2012). The Evolving Threat of Antimicrobial Resistance: Options for Action, World Health Organization."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.trsl.2020.06.001","article-title":"Antisense antibacterial compounds","volume":"223","author":"Pifer","year":"2020","journal-title":"Transl. 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