{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T15:40:18Z","timestamp":1778254818517,"version":"3.51.4"},"reference-count":64,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T00:00:00Z","timestamp":1628726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Bacterial inactivation using bacteriophages (or phages) has emerged as an effective solution for bacterial infections, but the screening methods used to evaluate the effectiveness of the phages to inactivate bacteria are not fast, reliable or precise enough. The efficiency of bacterial inactivation by phages has been evaluated by monitoring bacterial concentration either by counting colony-forming units (CFU), a laborious and time-consuming method, or by monitoring the optical density (OD), a less sensitive method. In this study, the resazurin cell viability assay was used to monitor the viability of bacteria from different genera during the inactivation by different phages, and the results were compared with the standard methods used to assess bacterial inactivation. The results showed that the resazurin colorimetric cell viability assay produces similar results to the standard method of colony-counting and giving, and also more sensitive results than the OD method. The resazurin assay can be used to quickly obtain the results of the cell viability effect profile using two different bacterial strains and several different phages at the same time, which is extremely valuable in screening studies. Moreover, this methodology is established as an effective, accurate and rapid method when compared to the ones widely used to monitor bacterial inactivation mediated by phages.<\/jats:p>","DOI":"10.3390\/antibiotics10080974","type":"journal-article","created":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T22:14:36Z","timestamp":1628806476000},"page":"974","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Application of the Resazurin Cell Viability Assay to Monitor Escherichia coli and Salmonella Typhimurium Inactivation Mediated by Phages"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4025-442X","authenticated-orcid":false,"given":"Pedro","family":"Costa","sequence":"first","affiliation":[{"name":"Department of Biology and CESAM, Campus Universit\u00e1rio de Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3201-0081","authenticated-orcid":false,"given":"Ana T. P. C.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, Campus Universit\u00e1rio de Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"M\u00e1rcia","family":"Braz","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, Campus Universit\u00e1rio de Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9209-7687","authenticated-orcid":false,"given":"Carla","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, Campus Universit\u00e1rio de Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8422-8664","authenticated-orcid":false,"given":"Adelaide","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, Campus Universit\u00e1rio de Santiago, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.virusres.2016.04.020","article-title":"Bacteriophages with potential to inactivate Salmonella Typhimurium: Use of single phage suspensions and phage cocktails","volume":"220","author":"Pereira","year":"2016","journal-title":"Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.fm.2016.09.003","article-title":"Application of phage therapy during bivalve depuration improves Escherichia coli decontamination","volume":"61","author":"Pereira","year":"2017","journal-title":"Food Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.aquaculture.2013.09.028","article-title":"Reductions of Vibrio parahaemolyticus in oysters after bacteriophage application during depuration","volume":"418\u2013419","author":"Rong","year":"2014","journal-title":"Aquaculture"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2016.00474","article-title":"Biocontrol and rapid detection of food-borne pathogens using bacteriophages and endolysins","volume":"7","author":"Bai","year":"2016","journal-title":"Front. 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