{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T17:53:28Z","timestamp":1773338008004,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T00:00:00Z","timestamp":1635206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Acinetobacter pittii is a species that belong to the Acinetobacter calcoaceticus-baumannii complex, increasingly recognized as major nosocomial bacterial pathogens, often associated with multiple drug-resistances. The capsule surrounding the bacteria represents a main virulence factor, helping cells avoid phage predation and host immunity. Accordingly, a better understanding of the phage infection mechanisms is required to efficiently develop phage therapy against Acinetobacter of different capsular types. Here, we report the isolation of the novel A. pittii-infecting Fri1-like phage vB_Api_3043-K38 (3043-K38) of the Podoviridae morphotype, from sewage samples. Its 41,580 bp linear double-stranded DNA genome harbours 53 open reading frames and 302 bp of terminal repeats. We show that all studied Acinetobacter Fri1-like viruses have highly similar genomes, which differentiate only at the genes coding for tailspike, likely to adapt to different host receptors. The isolated phage 3043-K38 specifically recognizes an untapped Acinetobacter K38 capsule type via a novel tailspike with K38 depolymerase activity. The recombinant K38 depolymerase region of the tailspike (center-end region) forms a thermostable trimer, and quickly degrades capsules. When the K38 depolymerase is applied to the cells, it makes them resistant to phage predation. Interestingly, while K38 depolymerase treatments do not synergize with antibiotics, it makes bacterial cells highly susceptible to the host serum complement. In summary, we characterized a novel phage-encoded K38 depolymerase, which not only advances our understanding of phage-host interactions, but could also be further explored as a new antibacterial agent against drug-resistant Acinetobacter.<\/jats:p>","DOI":"10.3390\/antibiotics10111304","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T23:49:52Z","timestamp":1635292192000},"page":"1304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Unpuzzling Friunavirus-Host Interactions One Piece at a Time: Phage Recognizes Acinetobacter pittii via a New K38 Capsule Depolymerase"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0762-816X","authenticated-orcid":false,"given":"Rita","family":"Domingues","sequence":"first","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]},{"given":"Ana","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0801-8080","authenticated-orcid":false,"given":"S\u00edlvio B.","family":"Santos","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]},{"given":"Diana Priscila","family":"Pires","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4556-6756","authenticated-orcid":false,"given":"Jonathan","family":"Save","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Medicine, Inselspital, Bern University Hospital, University of Bern, CH-3010 Bern, Switzerland"},{"name":"Centre for Research and Innovation in Clinical Pharmaceutical Sciences, Lausanne University Hospital, CH-1011 Lausanne, Switzerland"}]},{"given":"Gr\u00e9gory","family":"Resch","sequence":"additional","affiliation":[{"name":"Centre for Research and Innovation in Clinical Pharmaceutical Sciences, Lausanne University Hospital, CH-1011 Lausanne, Switzerland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5180-7133","authenticated-orcid":false,"given":"Joana","family":"Azeredo","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9564-5714","authenticated-orcid":false,"given":"Hugo","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Vrancianu, C.O., Gheorghe, I., Czobor, I.B., and Chifiriuc, M.C. 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