{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T20:59:41Z","timestamp":1776373181973,"version":"3.51.2"},"reference-count":80,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T00:00:00Z","timestamp":1639440000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\/MCTES (PIDDAC)","award":["Base Funding - UIDB\/00511\/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy \u2013 LEPABE - funded by national funds"],"award-info":[{"award-number":["Base Funding - UIDB\/00511\/2020 of the Laboratory for Process Engineering, Environment, Biotechnology and Energy \u2013 LEPABE - funded by national funds"]}]},{"name":"FCT","award":["Project PTDC\/CTMCOM\/4844\/2020"],"award-info":[{"award-number":["Project PTDC\/CTMCOM\/4844\/2020"]}]},{"name":"FCT","award":["Scientific Employment Stimulus-Individual Call-[CEEC-IND\/01700\/2017]"],"award-info":[{"award-number":["Scientific Employment Stimulus-Individual Call-[CEEC-IND\/01700\/2017]"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The low efficacy of conventional treatments and the interest in finding natural-based approaches to counteract biofilm development on urinary tract devices have promoted the research on probiotics. This work evaluated the ability of two probiotic strains, Lactobacillus plantarum and Lactobacillus rhamnosus, in displacing pre-formed biofilms of Escherichia coli and Staphylococcus aureus from medical-grade silicone. Single-species biofilms of 24 h were placed in contact with each probiotic suspension for 6 h and 24 h, and the reductions in biofilm cell culturability and total biomass were monitored by counting colony-forming units and crystal violet assay, respectively. Both probiotics significantly reduced the culturability of E. coli and S. aureus biofilms, mainly after 24 h of exposure, with reduction percentages of 70% and 77% for L. plantarum and 76% and 63% for L. rhamnosus, respectively. Additionally, the amount of E. coli biofilm determined by CV staining was maintained approximately constant after 6 h of probiotic contact and significantly reduced up to 67% after 24 h. For S. aureus, only L. rhamnosus caused a significant effect on biofilm amount after 6 h of treatment. Hence, this study demonstrated the potential of lactobacilli to control the development of pre-established uropathogenic biofilms.<\/jats:p>","DOI":"10.3390\/antibiotics10121525","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T09:34:25Z","timestamp":1639474465000},"page":"1525","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Using Lactobacilli to Fight Escherichia coli and Staphylococcus aureus Biofilms on Urinary Tract Devices"],"prefix":"10.3390","volume":"10","author":[{"given":"F\u00e1bio M.","family":"Carvalho","sequence":"first","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5233-1037","authenticated-orcid":false,"given":"Filipe J. M.","family":"Mergulh\u00e3o","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8992-1097","authenticated-orcid":false,"given":"Luciana C.","family":"Gomes","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,14]]},"reference":[{"key":"ref_1","unstructured":"(2021, September 20). Centers for Disease Control and Prevention Catheter-associated Urinary Tract Infections (CAUTI)|HAI|CDC, Available online: https:\/\/www.cdc.gov\/hai\/ca_uti\/uti.html."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/nrurol.2012.68","article-title":"New strategies to prevent catheter-associated urinary tract infections","volume":"9","author":"Siddiq","year":"2012","journal-title":"Nat. Rev. Urol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"7624857","DOI":"10.1155\/2018\/7624857","article-title":"Catheter-Associated Urinary Tract Infection and Obstinate Biofilm Producers","volume":"2018","author":"Maharjan","year":"2018","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref_4","unstructured":"(2021, September 20). World Health Organization Report on the Burden of Endemic Health Care-Associated Infection Worldwide. Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/80135."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e1800384","DOI":"10.1002\/mabi.201800384","article-title":"Evaluating efficacy of antimicrobial and antifouling materials for urinary tract medical devices: Challenges and recommendations","volume":"19","author":"Ramstedt","year":"2019","journal-title":"Macromol. Biosci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1099\/jmm.0.000032","article-title":"Healthcare-associated infections, medical devices and biofilms: Risk, tolerance and control","volume":"64","author":"Percival","year":"2015","journal-title":"J. Med. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e01067","DOI":"10.1016\/j.heliyon.2018.e01067","article-title":"Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention","volume":"4","author":"Khatoon","year":"2018","journal-title":"Heliyon"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lima, M., Teixeira-Santos, R., Gomes, L.C., Faria, S.I., Valcarcel, J., V\u00e1zquez, J.A., Cerqueira, M.A., Pastrana, L., Bourbon, A.I., and Mergulh\u00e3o, F.J. (2021). Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. Molecules, 26.","DOI":"10.3390\/molecules26144378"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1007\/s40506-016-0069-5","article-title":"New Technologies for Prevention of Catheter Associated Urinary Tract Infection","volume":"8","author":"Mandakhalikar","year":"2016","journal-title":"Curr. Treat. Options Infect. Dis."},{"key":"ref_10","first-page":"195","article-title":"Infection associated with medical devices","volume":"31","author":"Tunney","year":"2002","journal-title":"Int. J. Gen. Syst."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3858","DOI":"10.1021\/ac2029997","article-title":"Analytical challenges of microbial biofilms on medical devices","volume":"84","author":"Vertes","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1080\/1040841X.2016.1240656","article-title":"Impact of polymicrobial biofilms in catheter-associated urinary tract infections","volume":"43","author":"Azevedo","year":"2017","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3201\/eid0702.010226","article-title":"Biofilms and device-associated infections","volume":"7","author":"Donlan","year":"2001","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"101","DOI":"10.12816\/0004967","article-title":"Biofilm Formation by E. coli Causing Catheter Associated Urinary Tract Infection ( CAUTI ) in Assiut University Hospital","volume":"22","author":"Ahmed","year":"2013","journal-title":"Egypt. J. Med. Microbiol."},{"key":"ref_15","first-page":"1478","article-title":"The isolation and the biofilm formation of uropathogens in the patients with catheter associated urinary tract infections (UTIs)","volume":"6","author":"Niveditha","year":"2012","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.actbio.2017.01.011","article-title":"Probiotic E. coli Nissle 1917 biofilms on silicone substrates for bacterial interference against pathogen colonization","volume":"50","author":"Chen","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_17","first-page":"a000398","article-title":"Biofilms","volume":"2","author":"Vlamakis","year":"2010","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"881","DOI":"10.3201\/eid0809.020063","article-title":"Biofilms: Microbial life on surfaces","volume":"8","author":"Donlan","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1038\/nrmicro2415","article-title":"The biofilm matrix","volume":"8","author":"Flemming","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"493","DOI":"10.4155\/fmc.15.6","article-title":"Biofilm formation mechanisms and targets for developing antibiofilm agents","volume":"7","author":"Rabin","year":"2015","journal-title":"Future Med. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1128\/IAI.69.3.1322-1328.2001","article-title":"Biofilm formation in a hydrodynamic environment by novel FimH variants and ramifications for virulence","volume":"69","author":"Schembri","year":"2001","journal-title":"Infect. Immun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.medengphy.2004.12.013","article-title":"Materials for urinary catheters: A review of their history and development in the UK","volume":"27","author":"Lawrence","year":"2005","journal-title":"Med. Eng. Phys."},{"key":"ref_23","first-page":"1618","article-title":"A Novel Device-Integrated Drug Delivery System for Local Inhibition of Urinary Tract Infection","volume":"12","author":"Palarasah","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"18488","DOI":"10.3390\/ijms140918488","article-title":"Novel strategies for the prevention and treatment of biofilm related infections","volume":"14","author":"Chen","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1002\/jbm.a.36561","article-title":"Antimicrobial strategies for urinary catheters","volume":"107","author":"Zhu","year":"2019","journal-title":"J. Biomed. Mater. Res.\u2014Part A"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.actbio.2016.11.070","article-title":"A review of the recent advances in antimicrobial coatings for urinary catheters","volume":"50","author":"Singha","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_27","unstructured":"AGN FAO, Nutrition and Consumer Protection Div, and WHO, Geneva (2006). FAO Probiotics in Food: Health and Nutritional Properties and Guidelines for Evaluation, FAO."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S1521-6918(03)00075-1","article-title":"Probiotics: What are they? What are their effects on gut physiology?","volume":"17","author":"Fioramonti","year":"2003","journal-title":"Best Pract. Res. Clin. Gastroenterol."},{"key":"ref_29","first-page":"101","article-title":"Probiotics: Mechanisms of action and clinical applications","volume":"1","author":"Gogineni","year":"2013","journal-title":"J. Probiotics Heal."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.fm.2015.04.009","article-title":"Biofilms of Lactobacillus plantarum and Lactobacillus fermentum: Effect on stress responses, antagonistic effects on pathogen growth and immunomodulatory properties","volume":"53","author":"Aoudia","year":"2016","journal-title":"Food Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.anaerobe.2012.01.007","article-title":"Fructooligosaccharides metabolism and effect on bacteriocin production in Lactobacillus strains isolated from ensiled corn and molasses","volume":"18","author":"Mosquera","year":"2012","journal-title":"Anaerobe"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1016\/j.biotechadv.2018.09.003","article-title":"How to select a probiotic? A review and update of methods and criteria","volume":"36","author":"Soccol","year":"2018","journal-title":"Biotechnol. Adv."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1080\/1040-840291046759","article-title":"The lactic acid bacteria: A literature survey","volume":"28","author":"Carr","year":"2002","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.bj.2017.06.008","article-title":"Probiotic potential of lactobacilli with antagonistic activity against pathogenic strains: An in vitro validation for the production of inhibitory substances","volume":"40","author":"Prabhurajeshwar","year":"2017","journal-title":"Biomed. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1159\/000342079","article-title":"Probiotic mechanisms of action","volume":"61","author":"Gil","year":"2012","journal-title":"Ann. Nutr. Metab."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1002\/ibd.20602","article-title":"Mechanisms of action of probiotics: Recent advances","volume":"15","author":"Ng","year":"2009","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_37","first-page":"63646","article-title":"Probiotics: A comprehensive review of their classification, mode of action and role in human nutrition","volume":"10","author":"Khalighi","year":"2016","journal-title":"Probiotics Prebiotics Hum. Nutr. Health."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jgar.2019.02.021","article-title":"Inhibiting bacterial colonization on catheters: Antibacterial and antibiofilm activities of bacteriocins from Lactobacillus plantarum SJ33","volume":"19","author":"Jeevaratnam","year":"2019","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Hassl\u00f6f, P., Hedberg, M., Twetman, S., and Stecks\u00e9n-Blicks, C. (2010). Growth inhibition of oral mutans streptococci and candida by commercial probiotic lactobacilli\u2014An in vitro study. BMC Oral Health, 10.","DOI":"10.1186\/1472-6831-10-18"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.3906\/sag-1505-51","article-title":"The inhibitory effect of bacteriocin produced by Lactobacillus acidophilus ATCC 4356 and Lactobacillus plantarum ATCC 8014 on planktonic cells and biofilms of Serratia marcescens","volume":"46","author":"Ghadam","year":"2016","journal-title":"Turkish J. Med. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s12934-015-0283-8","article-title":"Inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming Lactobacillus isolate: A potential host for the expression of heterologous proteins","volume":"14","author":"Jalilsood","year":"2015","journal-title":"Microb. Cell Fact."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Carvalho, F.M., Teixeira-Santos, R., Mergulh\u00e3o, F.J.M., and Gomes, L.C. (2021). Effect of Lactobacillus plantarum Biofilms on the Adhesion of Escherichia coli to Urinary Tract Devices. Antibiotics, 10.","DOI":"10.3390\/antibiotics10080966"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Sambanthamoorthy, K., Feng, X., Patel, R., Patel, S., and Paranavitana, C. (2014). Antimicrobial and antibiofilm potential of biosurfactants isolated from lactobacilli against multi-drug-resistant pathogens. BMC Microbiol., 14.","DOI":"10.1186\/1471-2180-14-197"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"120","DOI":"10.3389\/fcimb.2018.00120","article-title":"Anti-biofilm properties of the fecal probiotic lactobacilli against Vibrio spp.","volume":"8","author":"Kaur","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.anireprosci.2005.11.004","article-title":"Inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus gasseri isolated from the vaginal tract of cattle","volume":"96","author":"Otero","year":"2006","journal-title":"Anim. Reprod. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"659","DOI":"10.2147\/IDR.S232982","article-title":"The battle of probiotics and their derivatives against biofilms","volume":"13","author":"Barzegari","year":"2020","journal-title":"Infect. Drug Resist."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Carvalho, F.M., Teixeira-Santos, R., Mergulh\u00e3o, F.J.M., and Gomes, L.C. (2021). The use of probiotics to fight biofilms in medical devices: A systematic review and meta-analysis. Microorganisms, 9.","DOI":"10.3390\/microorganisms9010027"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"501","DOI":"10.3934\/matersci.2021031","article-title":"Targeting biofilms in medical devices using probiotic cells: A systematic review","volume":"8","author":"Carvalho","year":"2021","journal-title":"AIMS Mater. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1472985","DOI":"10.1080\/20002297.2018.1472985","article-title":"Antimicrobial and anti-biofilm activities of Lactobacillus kefiranofaciens DD2 against oral pathogens","volume":"10","author":"Jeong","year":"2018","journal-title":"J. Oral Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0927-7765(02)00147-9","article-title":"Potential application of polyisobutylene-polystyrene and a Lactobacillus protein to reduce the risk of device-associated urinary tract infections","volume":"28","author":"Cadieux","year":"2003","journal-title":"Colloids Surf B Biointerfaces"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/0964-8305(95)00011-9","article-title":"Use of lactobacilli to reduce the adhesion of Staphylococcus aureus to catheters","volume":"34","author":"Reid","year":"1994","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1002\/jbm.a.35277","article-title":"Escherichia coli adhesion, biofilm development and antibiotic susceptibility on biomedical materials","volume":"103","author":"Gomes","year":"2015","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.bej.2016.11.013","article-title":"An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium","volume":"118","author":"Azevedo","year":"2017","journal-title":"Biochem. Eng. J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.archoralbio.2017.10.002","article-title":"Inhibitory effect of probiotic lactobacilli supernatants on single and mixed non-albicans Candida species biofilm","volume":"85","author":"Tan","year":"2018","journal-title":"Arch. Oral Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"6415","DOI":"10.1007\/s00253-016-7527-3","article-title":"Probiotic lactobacilli inhibit early stages of Candida albicans biofilm development by reducing their growth, cell adhesion, and filamentation","volume":"100","author":"Matsubara","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1080\/08927014.2018.1425402","article-title":"Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: Identification of potential probiotic candidates to prevent oral candidiasis","volume":"34","author":"Rossoni","year":"2018","journal-title":"Biofouling"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.ijfoodmicro.2015.04.030","article-title":"Characterisation of biofilms formed by Lactobacillus plantarum WCFS1 and food spoilage isolates","volume":"207","author":"Smid","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Jaffar, N., Ishikawa, Y., Mizuno, K., Okinaga, T., and Maeda, T. (2016). Mature biofilm degradation by potential probiotics: Aggregatibacter actinomycetemcomitans versus Lactobacillus spp.. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0159466"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.archoralbio.2016.12.014","article-title":"Inhibitory effects of Lactobacillus rhamnosus and Lactobacillus casei on Candida biofilm of denture surface","volume":"76","author":"Song","year":"2017","journal-title":"Arch. Oral Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"6008","DOI":"10.1128\/AEM.71.10.6008-6013.2005","article-title":"pH-, lactic acid-, and non-lactic acid-dependent activities of probiotic lactobacilli against Salmonella enterica serovar typhimurium","volume":"71","author":"Berger","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4175","DOI":"10.12659\/MSM.902237","article-title":"Effect of probiotic lactobacilli on the growth of Streptococcus mutans and multispecies biofilms isolated from children with active caries","volume":"23","author":"Lin","year":"2017","journal-title":"Med. Sci. Monit."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.fm.2012.08.009","article-title":"Induction of bacteriocin production by coculture is widespread among plantaricin-producing Lactobacillus plantarum strains with different regulatory operons","volume":"33","year":"2013","journal-title":"Food Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0300-9084(88)90206-4","article-title":"Bacteriocins of lactic acid bacteria","volume":"70","author":"Klaenhammer","year":"1988","journal-title":"Biochimie"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.colsurfb.2011.03.016","article-title":"Disruption of urogenital biofilms by lactobacilli","volume":"86","author":"McMillan","year":"2011","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_65","first-page":"13","article-title":"Lactobacillus by-products inhibit the growth and virulence of uropathogenic Escherichia coli","volume":"60","author":"Cadieux","year":"2009","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1186\/s12934-017-0769-7","article-title":"Biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65","volume":"16","author":"Morais","year":"2017","journal-title":"Microb. Cell Fact."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1111\/jam.12760","article-title":"Inhibition of Candida albicans adhesion on medical-grade silicone by a Lactobacillus-derived biosurfactant","volume":"118","author":"Ceresa","year":"2015","journal-title":"J. Appl. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.btre.2016.05.001","article-title":"Functional characterization of biomedical potential of biosurfactant produced by Lactobacillus helveticus","volume":"11","author":"Sharma","year":"2016","journal-title":"Biotechnol. Reports"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1007\/s10529-019-02694-2","article-title":"Lactobacillus rhamnosus GG microcapsules inhibit Escherichia coli biofilm formation in coculture","volume":"41","author":"Song","year":"2019","journal-title":"Biotechnol. Lett."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Ahn, K.B., Baik, J.E., Park, O.J., Yun, C.H., and Han, S.H. (2018). Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0192694"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.joen.2018.12.007","article-title":"Lactobacillus plantarum lipoteichoic acid inhibits oral multispecies biofilm","volume":"45","author":"Kim","year":"2019","journal-title":"J. Endod."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1111\/j.1472-765X.2011.03132.x","article-title":"Inhibitory effect of oral Lactobacillus against oral pathogens","volume":"53","author":"Teanpaisan","year":"2011","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Alexandre, Y., Le Berre, R., Barbier, G., and Le Blay, G. (2014). Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections. BMC Microbiol., 14.","DOI":"10.1186\/1471-2180-14-107"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1080\/13102818.2000.10819062","article-title":"Influence of growth medium on bacteriocin production in Lactobacillus plantarum ST31","volume":"14","author":"Todorov","year":"2000","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1046\/j.1472-765X.1997.00378.x","article-title":"A simple artificial urine for the growth of urinary pathogens","volume":"24","author":"Brooks","year":"1997","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1093\/jac\/dkl024","article-title":"Biosurfactants: Potential applications in medicine","volume":"57","author":"Rodrigues","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Ghista, D.N. (2012). Biosurfactants and Bioemulsifiers Biomedical and Related Applications\u2014Present Status and Future Potentials. Biomedical Science, Engineering and Technology, InTech Publisher.","DOI":"10.5772\/1020"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1080\/08927014.2013.807913","article-title":"Biofilm formation with mixed cultures of Pseudomonas aeruginosa\/Escherichia coli on silicone using artificial urine to mimic urinary catheters","volume":"29","author":"Cerqueira","year":"2013","journal-title":"Biofouling"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"4407","DOI":"10.1128\/AEM.67.10.4407-4413.2001","article-title":"Growth of the Bacteriocin-Producing Lactobacillus sakei Strain CTC 494 in MRS Broth is Strongly Reduced Due to Nutrient Exhaustion: A Nutrient Depletion Model for the Growth of Lactic Acid Bacteria","volume":"67","author":"Leroy","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Gomes, M., Gomes, L.C., Teixeira-Santos, R., Pereira, M.F.R., Soares, O.S.G.P., and Mergulh\u00e3o, F.J. (2021). Optimizing CNT Loading in Antimicrobial Composites for Urinary Tract Application. Appl. 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