{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T23:27:00Z","timestamp":1771889220027,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT, Portugal)","doi-asserted-by":"publisher","award":["UID\/04413\/2020"],"award-info":[{"award-number":["UID\/04413\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT, Portugal)","doi-asserted-by":"publisher","award":["LA\/P\/0117\/2020"],"award-info":[{"award-number":["LA\/P\/0117\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT, Portugal)","doi-asserted-by":"publisher","award":["2022.07931.PTDC"],"award-info":[{"award-number":["2022.07931.PTDC"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT, Portugal)","doi-asserted-by":"publisher","award":["UI\/BD\/154472\/2022"],"award-info":[{"award-number":["UI\/BD\/154472\/2022"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT, Portugal)","doi-asserted-by":"publisher","award":["CEECINST\/00102\/2018\/CP1567\/CT0040"],"award-info":[{"award-number":["CEECINST\/00102\/2018\/CP1567\/CT0040"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT, Portugal)","doi-asserted-by":"publisher","award":["CEECINST\/00042\/2021\/CP1773\/CT0009"],"award-info":[{"award-number":["CEECINST\/00042\/2021\/CP1773\/CT0009"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Antimicrobial-resistant Staphylococcus aureus is a growing threat to human health for which alternative therapeutic options are needed. In this study, we aimed to evaluate the efficacy of amlodipine (AML) and imipramine (IMI) to treat S. aureus infection in the Galleria mellonella larval model by targeting efflux and biofilms, which are relevant contributors to antimicrobial resistance and virulence in S. aureus. Methods: In-house reared G. mellonella were used in virulence assays to determine the infective dose of two S. aureus strains differing in the expression of norA (gene encoding the native NorA efflux pump). Toxicology assays were conducted to determine the drugs\u2019 LD50 for G. mellonella. Drug efficacy assays were performed to evaluate the potential of amlodipine, imipramine and the control drugs ciprofloxacin (CIP) and enalapril (ENA) to clear S. aureus infection in G. mellonella. Results: Survival analysis defined the infective dose as 1 \u00d7 107 CFU\/larva for both strains. High LD50 values were determined (CIP: &gt;1000 mg\/kg; AML: &gt;640 mg\/kg; IMI: 1141 mg\/kg; ENA: &gt;1280 mg\/kg), revealing a high tolerance of G. mellonella to these drugs. AML at 15 mg\/kg and IMI at 100 mg\/kg increased the larvae survival by 20% (p = 0.04) and 11% (p = 0.11), respectively, also positively affecting health score indexes. In agreement with the literature, ciprofloxacin at &gt;100 mg\/kg promoted larvae survival by &gt;73%. Conclusions: Amlodipine and imipramine show mild potential as new therapeutic options for managing S. aureus infections but are promising as new lead molecules. This study also reinforces G. mellonella as a sustainable, reliable model for drug evaluation.<\/jats:p>","DOI":"10.3390\/antibiotics14020183","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T11:01:08Z","timestamp":1739271668000},"page":"183","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of Amlodipine and Imipramine Efficacy to Treat Galleria mellonella Infection by Biofilm-Producing and Antimicrobial-Resistant Staphylococcus aureus"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7085-4218","authenticated-orcid":false,"given":"Mariana","family":"Andrade","sequence":"first","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"given":"Joana","family":"Neves","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8736-1384","authenticated-orcid":false,"given":"Maria","family":"Bento","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"given":"Joana","family":"Marques","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"given":"Sofia G.","family":"Seabra","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7939-772X","authenticated-orcid":false,"given":"Henrique","family":"Silveira","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"given":"Liliana","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5066-9982","authenticated-orcid":false,"given":"Ana","family":"Armada","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9676-6251","authenticated-orcid":false,"given":"Miguel","family":"Viveiros","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3536-2733","authenticated-orcid":false,"given":"Isabel","family":"Couto","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4096-2410","authenticated-orcid":false,"given":"Sofia Santos","family":"Costa","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, Associated Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, 1349-008 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1038\/s41579-024-01054-w","article-title":"ESKAPE pathogens: Antimicrobial resistance, epidemiology, clinical impact and therapeutics","volume":"22","author":"Miller","year":"2024","journal-title":"Nat Rev Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/s41579-018-0147-4","article-title":"Methicillin-resistant Staphylococcus aureus: An overview of basic and clinical research","volume":"17","author":"Turner","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_3","unstructured":"Antimicrobial Resistance Collaborators (2022). Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis. Lancet, 400, 1102."},{"key":"ref_4","unstructured":"World Health Organization (2024). WHO Bacterial Priority Pathogens List: Bacterial Pathogens of Public Health Importance to Guide Research, Development and Strategies to Prevent and Control Antimicrobial Resistance, World Health Organization."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2174\/1874285801307010059","article-title":"Multidrug Efflux Pumps in Staphylococcus aureus: An Update","volume":"7","author":"Costa","year":"2013","journal-title":"Open. Microbiol. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"135253","DOI":"10.1016\/j.ijbiomac.2024.135253","article-title":"An escape from ESKAPE pathogens: A comprehensive review on current and emerging therapeutics against antibiotic resistance","volume":"279","author":"Singh","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1186\/s12866-015-0572-8","article-title":"Impact of efflux in the development of multidrug resistance phenotypes in Staphylococcus aureus","volume":"15","author":"Costa","year":"2015","journal-title":"BMC Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.2217\/fmb.14.66","article-title":"Multidrug efflux pumps in Gram-negative bacteria and their role in antibiotic resistance","volume":"9","author":"Blair","year":"2014","journal-title":"Future Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ferreira, C., Abrantes, P., Costa, S.S., Viveiros, M., and Couto, I. (2022). Occurrence and Variability of the Efflux Pump Gene norA across the Staphylococcus Genus. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232315306"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.drup.2016.04.003","article-title":"Multidrug efflux pumps of Gram-positive bacteria","volume":"27","author":"Schindler","year":"2016","journal-title":"Drug Resist. Updates"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ijantimicag.2012.04.014","article-title":"Expression of multidrug resistance efflux pump genes in clinical and environmental isolates of Staphylococcus aureus","volume":"40","author":"Kosmidis","year":"2012","journal-title":"Int. J. Antimicrob. Agents."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/microbiolspec.GPP3-0023-2018","article-title":"Staphylococcal Biofilms","volume":"6","author":"Otto","year":"2018","journal-title":"Microbiol. Spectr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1128\/MMBR.00013-14","article-title":"Biofilm-related infections: Bridging the gap between clinical management and fundamental aspects of recalcitrance toward antibiotics","volume":"78","author":"Lebeaux","year":"2014","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1093\/jac\/dky042","article-title":"Role of bacterial efflux pumps in biofilm formation","volume":"73","author":"Alav","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1315238","DOI":"10.3389\/fmicb.2024.1315238","article-title":"Efflux pumps as potential targets for biofilm inhibition","volume":"15","author":"Ren","year":"2024","journal-title":"Front. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1080\/08927014.2016.1232402","article-title":"Combinatorial approaches with selected phytochemicals to increase antibiotic efficacy against Staphylococcus aureus biofilms","volume":"32","author":"Abreu","year":"2016","journal-title":"Biofouling"},{"key":"ref_17","unstructured":"Neves, J., Andrade, M., Bento, M., Viveiros, M., Rodrigues, L., Couto, I., and Costa, S.S. (2024, January 19\u201321). New candidate drugs for repurposing as efflux inhibitors and antibiofilm agents against antimicrobial-resistant Staphylococcus aureus and Staphylococcus epidermidis. Proceedings of the 19th International Symposium on Staphylococci and Staphylococcal Infections, Perth, Australia. Available online: https:\/\/icmsmeetings.eventsair.com\/isssi-2024\/speakers."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"D1265","DOI":"10.1093\/nar\/gkad976","article-title":"DrugBank 6.0: The DrugBank Knowledgebase for 2024","volume":"52","author":"Knox","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"782733","DOI":"10.3389\/fcimb.2021.782733","article-title":"Galleria mellonella as a Suitable Model of Bacterial Infection: Past, Present and Future","volume":"11","author":"Rouillon","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1099\/mic.0.000813","article-title":"Utilization of Galleria mellonella larvae to characterize the development of Staphylococcus aureus infection","volume":"165","author":"Sheehan","year":"2019","journal-title":"Microbiology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2598","DOI":"10.1128\/IAI.00296-13","article-title":"The Dot\/Icm effector SdhA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A\/J mice","volume":"81","author":"Harding","year":"2013","journal-title":"Infect. Immun."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Moya-And\u00e9rico, L., Admella, J., Fernandes, R., and Torrents, E. (2020). Monitoring Gene Expression during a Galleria mellonella Bacterial Infection. Microorganisms, 8.","DOI":"10.3390\/microorganisms8111798"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1080\/21505594.2017.1347744","article-title":"Galleria mellonella as a host model to study Candida glabrata virulence and antifungal efficacy","volume":"8","author":"Ames","year":"2017","journal-title":"Virulence"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Piatek, M., Sheehan, G., and Kavanagh, K. (2021). Galleria mellonella: The Versatile Host for Drug Discovery, In Vivo Toxicity Testing and Characterising Host-Pathogen Interactions. Antibiotics, 10.","DOI":"10.3390\/antibiotics10121545"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Fletcher, M., McCormack, A., Parcell, B.J., and Coote, P.J. (2023). Combination Therapy with Ciprofloxacin and Pentamidine against Multidrug-Resistant Pseudomonas aeruginosa: Assessment of In Vitro and In Vivo Efficacy and the Role of Resistance-Nodulation-Division (RND) Efflux Pumps. Antibiotics, 12.","DOI":"10.3390\/antibiotics12081236"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1093\/jac\/dkn217","article-title":"Efflux-mediated response of Staphylococcus aureus exposed to ethidium bromide","volume":"62","author":"Couto","year":"2008","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Andrade, M., Oliveira, K., Morais, C., Abrantes, P., Pomba, C., Rosato, A.E., Couto, I., and Costa, S.S. (2022). Virulence Potential of Bio-film-Producing Staphylococcus pseudintermedius, Staphylococcus aureus and Staphylococcus coagulans Causing Skin Infections in Companion Animals. Antibiotics, 11.","DOI":"10.3390\/antibiotics11101339"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"10758","DOI":"10.1038\/s41598-024-61345-3","article-title":"Exploring the virulence potential of Staphylococcus aureus CC121 and CC152 lineages related to paediatric community-acquired bacteraemia in Manhi\u00e7a, Mozambique","volume":"14","author":"Garrine","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1186\/s13104-017-2757-8","article-title":"Galleria mellonella (greater wax moth) larvae as a model for antibiotic susceptibility testing and acute toxicity trials","volume":"10","author":"Ignasiak","year":"2017","journal-title":"BMC Res. Notes"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1080\/21505594.2021.1878688","article-title":"Pathogenicity and virulence of Staphylococcus aureus","volume":"12","author":"Cheung","year":"2021","journal-title":"Virulence"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"44280","DOI":"10.1039\/C7RA03859C","article-title":"Investigation on the effect of known potent S. aureus NorA efflux pump inhibitors on the staphylococcal biofilm formation","volume":"7","author":"Sabatini","year":"2017","journal-title":"RSC Adv."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/nrd.2018.168","article-title":"Drug repurposing: Progress, challenges and recommendations","volume":"18","author":"Pushpakom","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_33","first-page":"282","article-title":"Drug repurposing as an alternative for the treatment of recalcitrant bacterial infections","volume":"6","author":"Bernstein","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1080\/21505594.2019.1659663","article-title":"New solvent options for in vivo assays in the Galleria mellonella larvae model","volume":"10","year":"2019","journal-title":"Virulence"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.ejmech.2018.01.078","article-title":"Ciprofloxacin derivatives and their antibacterial activities","volume":"146","author":"Zhang","year":"2018","journal-title":"Eur. J. Med. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1054\/drup.1998.0068","article-title":"Mechanisms of fluoroquinolone resistance","volume":"2","author":"Hooper","year":"1999","journal-title":"Drug Resist. Updat."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1093\/jat\/30.5.346","article-title":"Two cases of fatal amlodipine overdose","volume":"30","author":"Sklerov","year":"2006","journal-title":"J. Anal. Toxicol."},{"key":"ref_38","unstructured":"(2025, January 27). IPCS Inchem. Available online: https:\/\/www.inchem.org\/documents\/pims\/pharm\/imiprami.htm#SectionTitle:4.2%20Therapeutic%20dosage."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"505","DOI":"10.2217\/fmb-2022-0230","article-title":"Activity of amlodipine against Staphylococcus aureus: Association with oxacillin and mechanism of action","volume":"18","author":"Barbosa","year":"2023","journal-title":"Future Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1128\/AAC.04516-14","article-title":"Targeting Mycobacterium tuberculosis topoisomerase I by small-molecule inhibitors","volume":"59","author":"Godbole","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"723133","DOI":"10.3389\/fmicb.2021.723133","article-title":"A Substituted Diphenyl Amide Based Novel Scaffold Inhibits Staphylococcus aureus Virulence in a Galleria mellonella Infection Model","volume":"12","author":"Mishra","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"685807","DOI":"10.3389\/fphar.2021.685807","article-title":"Synergistic Effect of Cefazolin Plus Fosfomycin Against Staphylococcus aureus in vitro and in vivo in an Experimental Galleria mellonella Model","volume":"12","author":"Kussmann","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1038\/s41598-024-51316-z","article-title":"Immunophenotyping of hemocytes from infected Galleria mellonella larvae as an innovative tool for immune profiling, infection studies and drug screening","volume":"14","author":"Gallorini","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1089\/adt.2016.752","article-title":"An experimental model for the rapid screening of compounds with potential use against mycobacteria","volume":"14","author":"Costa","year":"2016","journal-title":"ASSAY Drug Dev. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1080\/21505594.2017.1397871","article-title":"From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies","volume":"9","author":"Oliveira","year":"2018","journal-title":"Virulence"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"419","DOI":"10.4161\/viru.24930","article-title":"Galleria mellonella larvae as an infection model for group A Streptococcus","volume":"4","author":"Loh","year":"2013","journal-title":"Virulence"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1080\/21505594.2015.1135289","article-title":"Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing","volume":"7","author":"Tsai","year":"2016","journal-title":"Virulence"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s12250-020-00230-5","article-title":"On the Calculation of TCID50 for Quantitation of Virus Infectivity","volume":"36","author":"Lei","year":"2021","journal-title":"Virol. Sin."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/2\/183\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:31:31Z","timestamp":1760027491000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/2\/183"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,11]]},"references-count":48,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["antibiotics14020183"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics14020183","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,11]]}}}