{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:33:24Z","timestamp":1768520004619,"version":"3.49.0"},"reference-count":29,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["PATCH 022.05810.PTDC"],"award-info":[{"award-number":["PATCH 022.05810.PTDC"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDB\/00102\/2020"],"award-info":[{"award-number":["UIDB\/00102\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDP\/00102\/2020"],"award-info":[{"award-number":["UIDP\/00102\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Fungal infections have significant health risks due to virulence factors like biofilms that lead to chronic and persistent infections. To decrease associated rates of morbidity and mortality, it is crucial to develop effective antifungal treatments. The present study aims to evaluate the bioactive potential of Ferulago spp. essential oils by assessing their effect on dermatophytes and wound healing, as these fungi are often associated with wound infection. Methods: The minimal inhibitory concentration (MIC) and the minimal lethal concentration (MLC) of five essential oils were assessed on planktonic dermatophytes and the most promising were used to evaluate their effect on the formation and disruption of biofilms, by quantifying biofilm mass using crystal violet and extracellular matrix deposition using safranin staining. Alterations in fungal morphology were confirmed by optical microscopy and a cell migration assay was used to assess wound-healing capacity. Results: Ferulago silaifolia essential oil characterized by high amounts of \u03b1-pinene (45.4%) and cis-crysanthenyl acetate (39.1%) was the most active, particularly against Microsporum canis and Trichophyton rubrum (MIC = 50 \u00b5g\/mL). Regarding biofilm assays, Trichophyton rubrum was the most susceptible strain, with both biofilm mass and extracellular matrix being highly compromised with an evident decrease in hyphal growth and mycelial density. In addition, this essential oil significantly increased fibroblast migration at 25 \u03bcg\/mL, indicating a wound-healing effect that could prevent systemic infections. Conclusions: The present study provides new insights into the treatment of dermatophytosis by highlighting the antivirulent effects of F. silaifolia essential oil and its potential wound-healing properties.<\/jats:p>","DOI":"10.3390\/antibiotics14040343","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T04:36:48Z","timestamp":1743136608000},"page":"343","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unlocking the Skin-Protective Effects of Ferulago spp. Essential Oils: A Focus on Antidermatophytic and Wound-Healing Potential"],"prefix":"10.3390","volume":"14","author":[{"given":"Ceyda Sibel","family":"K\u0131l\u0131\u00e7","sequence":"first","affiliation":[{"name":"Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Botany, 06560 Ankara, T\u00fcrkiye"}]},{"given":"In\u00eas","family":"Catarino","sequence":"additional","affiliation":[{"name":"Univ Coimbra, Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5270-3993","authenticated-orcid":false,"given":"Jorge","family":"Alves-Silva","sequence":"additional","affiliation":[{"name":"Univ Coimbra, Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"},{"name":"Univ Coimbra, Center for Innovative Biomedicine and Biotechnology (CIBB), 3000-548 Coimbra, Portugal"},{"name":"Univ Coimbra, Faculty of Pharmacy, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"},{"name":"Clinical Academic Centre of Coimbra (CACC), 3000-548 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2343-746X","authenticated-orcid":false,"given":"Bet\u00fcl","family":"Demirci","sequence":"additional","affiliation":[{"name":"Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, 26470 Tepeba\u015f\u0131, Eski\u015fehir, T\u00fcrkiye"}]},{"given":"Damla","family":"K\u0131rc\u0131","sequence":"additional","affiliation":[{"name":"\u0130zmir Katip \u00c7elebi University, Faculty of Pharmacy, Department of Pharmacognosy, 35620 \u00c7i\u011fli, \u0130zmir, T\u00fcrkiye"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0948-821X","authenticated-orcid":false,"given":"L\u00edgia","family":"Salgueiro","sequence":"additional","affiliation":[{"name":"Univ Coimbra, Faculty of Pharmacy, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"},{"name":"Univ Coimbra, Chemical Engineering and Renewable Resources for Sustainability (CERES), Department of Chemical Engineering, 3030-790 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4128-9010","authenticated-orcid":false,"given":"M\u00f3nica","family":"Zuzarte","sequence":"additional","affiliation":[{"name":"Univ Coimbra, Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"},{"name":"Univ Coimbra, Center for Innovative Biomedicine and Biotechnology (CIBB), 3000-548 Coimbra, Portugal"},{"name":"Univ Coimbra, Faculty of Pharmacy, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal"},{"name":"Clinical Academic Centre of Coimbra (CACC), 3000-548 Coimbra, Portugal"},{"name":"Univ Coimbra, Chemical Engineering and Renewable Resources for Sustainability (CERES), Department of Chemical Engineering, 3030-790 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"ref_1","unstructured":"(2024, December 01). Gaffi Global Action for Fungal Infections Fungal Disease Frequency. Available online: https:\/\/gaffi.org\/why\/fungi-fungal-infections\/."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Bongomin, F., Gago, S., Oladele, R., and Denning, D. (2017). Global and Multi-National Prevalence of Fungal Diseases\u2014Estimate Precision. J. Fungi, 3.","DOI":"10.3390\/jof3040057"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.3389\/fmicb.2018.01108","article-title":"Dermatophyte Resistance to Antifungal Drugs: Mechanisms and Prospectus","volume":"9","author":"Bitencourt","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1285887","DOI":"10.3389\/fimmu.2023.1285887","article-title":"Dermatophyte infection: From fungal pathogenicity to host immune responses","volume":"14","author":"Deng","year":"2023","journal-title":"Front. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1093\/mmy\/myx160","article-title":"Keratin hydrolysis by dermatophytes","volume":"57","author":"Mercer","year":"2019","journal-title":"Med. Mycol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Jartarkar, S.R., Patil, A., Goldust, Y., Cockerell, C.J., Schwartz, R.A., Grabbe, S., and Goldust, M. (2021). Pathogenesis, Immunology and Management of Dermatophytosis. J. Fungi, 8.","DOI":"10.3390\/jof8010039"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"77","DOI":"10.4103\/2229-5178.178099","article-title":"Management of tinea corporis, tinea cruris, and tinea pedis: A comprehensive review","volume":"7","author":"Sahoo","year":"2016","journal-title":"Indian Dermatol. Online J."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Markantonatou, A.-M., Samaras, K., and Vyzantiadis, T.-A. (2023). Dermatophytic Biofilms: Characteristics, Significance and Treatment Approaches. J. Fungi, 9.","DOI":"10.3390\/jof9020228"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1111\/j.1540-9740.2005.03435.x","article-title":"Dermatophytosis: The Management of Fungal Infections","volume":"4","author":"Aditya","year":"2005","journal-title":"SKINmed Dermatol. Clin."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1007\/s40199-024-00530-1","article-title":"A review of the ethnomedicinal, phytochemical, and pharmacological properties of the Ferulago genus based on Structure\u2013Activity Relationship (SAR) of coumarins","volume":"32","author":"Dabaghian","year":"2024","journal-title":"DARU J. Pharm. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"51","DOI":"10.5505\/tjps.2016.02886","article-title":"T\u00fcrkiye\u2019de Yeti\u015fen Ferulago blancheana Post. (Apiaceae) T\u00fcr\u00fcn\u00fcn Toprak \u00dcst\u00fc, \u00c7i\u00e7ek ve K\u00f6klerinden Elde Edilen U\u00e7ucu Ya\u011flar\u0131n \u0130\u00e7eriklerinin ve Antimikrobiyal Aktivitesinin Biyootografi Y\u00f6ntemiyle Tan\u0131mlanmas\u0131","volume":"13","author":"Karakaya","year":"2016","journal-title":"Turk. J. Pharm. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"114050","DOI":"10.1016\/j.jep.2021.114050","article-title":"The ethnobotany, phytochemistry and biological properties of genus Ferulago\u2013A review","volume":"274","author":"Badalamenti","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","first-page":"352","article-title":"The Genus Ferulago: A Review on Ethnopharmacology, Phytochemistry, and Pharmacology","volume":"20","author":"Rahimpour","year":"2021","journal-title":"Iran. J. Pharm. Res. IJPR"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1002\/ffj.2010","article-title":"Essential oil from fruits and roots of Ferulago campestris (Besser) Grecescu (Apiaceae): Composition and antioxidant and anti-Candida activity","volume":"25","author":"Cecchini","year":"2010","journal-title":"Flavour Fragr. J."},{"key":"ref_15","first-page":"18","article-title":"Chemical and antimicrobial characterization of essential oils obtained from aerial part, root and fruit of Ferulago longistylis Boiss., an endemic species","volume":"7","author":"Demirci","year":"2020","journal-title":"Nat. Volatiles Essent. Oils"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1007\/s10096-013-1881-1","article-title":"Antifungal activity of Ferulago capillaris essential oil against Candida, Cryptococcus, Aspergillus and dermatophyte species","volume":"32","author":"Pinto","year":"2013","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e202301098","DOI":"10.1002\/cbdv.202301098","article-title":"Essential Oil Composition of Fruits of Eight Ferulago Species Growing in T\u00fcrkiye and Multivariate Statistical Analyses","volume":"20","author":"Demirci","year":"2023","journal-title":"Chem. Biodivers."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.3109\/13880209.2016.1162816","article-title":"Chemical composition, antioxidant and antibacterial activities of essential oils from Ferulago angulata","volume":"54","author":"Izadi","year":"2016","journal-title":"Pharm. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Pandey, A.K., and Singh, P. (2017). The Genus Artemisia: A 2012\u20132017 Literature Review on Chemical Composition, Antimicrobial, Insecticidal and Antioxidant Activities of Essential Oils. Medicines, 4.","DOI":"10.3390\/medicines4030068"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s11418-014-0884-2","article-title":"Antifungal activity of the essential oil of Angelica major against Candida, Cryptococcus, Aspergillus and dermatophyte species","volume":"69","author":"Cavaleiro","year":"2015","journal-title":"J. Nat. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6190","DOI":"10.1080\/14786419.2020.1837803","article-title":"Antifungal action of \u03b1-pinene against Candida spp. isolated from patients with otomycosis and effects of its association with boric acid","volume":"35","author":"Silva","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e58711427748","DOI":"10.33448\/rsd-v11i4.27748","article-title":"Efeito antif\u00fangico de \u03b1-pineno isolado e em associa\u00e7\u00e3o com antif\u00fangicos frente \u00e0s cepas de Candida albicans","volume":"11","author":"Lima","year":"2022","journal-title":"Res. Soc. Dev."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Alves-Silva, J., Zuzarte, M., Cavaleiro, C., and Salgueiro, L. (2023). Antibiofilm Effect of Lavandula multifida Essential Oil: A New Approach for Chronic Infections. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15082142"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e22210207","DOI":"10.1590\/1678-4324-2022210207","article-title":"In Vitro Enzyme Inhibitory Activity of Ten Ferulago W. Koch Species Growing in Turkey","volume":"65","author":"Akkol","year":"2022","journal-title":"Braz. Arch. Biol. Technol."},{"key":"ref_25","first-page":"1","article-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard. Second Edition. M38-A2","volume":"28","author":"HR","year":"2008","journal-title":"Clin. Lab. Stand. Inst."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1080\/08927014.2020.1811856","article-title":"In vitro and ex vivo biofilms of dermatophytes: A new panorama for the study of antifungal drugs","volume":"36","author":"Lopes","year":"2020","journal-title":"Biofouling"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1080\/08927014.2014.919282","article-title":"In vitro characterization of Trichophyton rubrum and T. mentagrophytes biofilms","volume":"30","author":"Sardi","year":"2014","journal-title":"Biofouling"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Martinotti, S., and Ranzato, E. (2019). Scratch Wound Healing Assay. Epidermal Cells: Methods and Protocols, Humana.","DOI":"10.1007\/7651_2019_259"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e0232565","DOI":"10.1371\/journal.pone.0232565","article-title":"An image J plugin for the high throughput image analysis of in vitro scratch wound healing assays","volume":"15","author":"Clavijo","year":"2020","journal-title":"PLoS ONE"}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/4\/343\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:02:59Z","timestamp":1760029379000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/14\/4\/343"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,27]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["antibiotics14040343"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics14040343","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,27]]}}}