{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:59:31Z","timestamp":1777481971226,"version":"3.51.4"},"reference-count":56,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/BIO\/04469\/2013"],"award-info":[{"award-number":["UID\/BIO\/04469\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BBB-BSS\/6471\/2014"],"award-info":[{"award-number":["PTDC\/BBB-BSS\/6471\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-006684"],"award-info":[{"award-number":["POCI-01-0145-FEDER-006684"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PD\/BD\/135254\/2017"],"award-info":[{"award-number":["PD\/BD\/135254\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000004"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000004"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens and are commonly found in polymicrobial biofilm-associated diseases, namely chronic wounds. Their co-existence in a biofilm contributes to an increased tolerance of the biofilm to antibiotics. Combined treatments of bacteriophages and antibiotics have shown a promising antibiofilm activity, due to the profound differences in their mechanisms of action. In this study, 48 h old mono and dual-species biofilms were treated with a newly isolated P. aeruginosa infecting phage (EPA1) and seven different antibiotics (gentamicin, kanamycin, tetracycline, chloramphenicol, erythromycin, ciprofloxacin, and meropenem), alone and in simultaneous or sequential combinations. The therapeutic efficacy of the tested antimicrobials was determined. Phage or antibiotics alone had a modest effect in reducing biofilm bacteria. However, when applied simultaneously, a profound improvement in the killing effect was observed. Moreover, an impressive biofilm reduction (below the detection limit) was observed when gentamicin or ciprofloxacin were added sequentially after 6 h of phage treatment. The effect observed does not depend on the type of antibiotic but is influenced by its concentration. Moreover, in dual-species biofilms it was necessary to increase gentamicin concentration to obtain a similar killing effect as occurs in mono-species. Overall, combining phages with antibiotics can be synergistic in reducing the bacterial density in biofilms. However, the concentration of antibiotic and the time of antibiotic application are essential factors that need to be considered in the combined treatments.<\/jats:p>","DOI":"10.3390\/antibiotics8030103","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T08:45:39Z","timestamp":1564130739000},"page":"103","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":158,"title":["Synergistic Action of Phage and Antibiotics: Parameters to Enhance the Killing Efficacy Against Mono and Dual-Species Biofilms"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6294-6948","authenticated-orcid":false,"given":"Ergun","family":"Akturk","sequence":"first","affiliation":[{"name":"LIBRO-Laborat\u00f3rio de Investiga\u00e7\u00e3o em Biofilmes Ros\u00e1rio Oliveira, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4700-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9564-5714","authenticated-orcid":false,"given":"Hugo","family":"Oliveira","sequence":"additional","affiliation":[{"name":"LIBRO-Laborat\u00f3rio de Investiga\u00e7\u00e3o em Biofilmes Ros\u00e1rio Oliveira, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4700-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0801-8080","authenticated-orcid":false,"given":"S\u00edlvio B.","family":"Santos","sequence":"additional","affiliation":[{"name":"LIBRO-Laborat\u00f3rio de Investiga\u00e7\u00e3o em Biofilmes Ros\u00e1rio Oliveira, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4700-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2661-3617","authenticated-orcid":false,"given":"Susana","family":"Costa","sequence":"additional","affiliation":[{"name":"LIBRO-Laborat\u00f3rio de Investiga\u00e7\u00e3o em Biofilmes Ros\u00e1rio Oliveira, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4700-057 Braga, Portugal"}]},{"given":"Suleyman","family":"Kuyumcu","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Medical Faculty, Sifa University, 35535 Izmir, Turkey"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6865-6044","authenticated-orcid":false,"given":"Lu\u00eds D. 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Wound Care"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1111\/joim.12004","article-title":"Biofilm infections, their resilience to therapy and innovative treatment strategies","volume":"272","author":"Balsalobre","year":"2012","journal-title":"J. Intern. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1093\/femsre\/fux010","article-title":"Molecular mechanisms of biofilm-based antibiotic resistance and tolerance in pathogenic bacteria","volume":"41","author":"Hall","year":"2017","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.ijantimicag.2012.04.020","article-title":"Antibiotic resistance of mixed biofilms in cystic fibrosis: Impact of emerging microorganisms on treatment of infection","volume":"40","author":"Lopes","year":"2012","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcimb.2017.00106","article-title":"In vivo and In vitro Interactions between Pseudomonas aeruginosa and Staphylococcus spp.","volume":"7","author":"Hotterbeekx","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1111\/j.1365-2672.2008.04027.x","article-title":"Enhanced biofilm formation and 3-chlorobenzoate degrading activity by the bacterial consortium of Burkholderia sp. NK8 and Pseudomonas aeruginosa PAO1","volume":"106","author":"Yoshida","year":"2009","journal-title":"J. Appl. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pastar, I., Nusbaum, A.G., Gil, J., Patel, S.B., Chen, J., Valdes, J., Stojadinovic, O., Plano, L.R., Tomic-Canic, M., and Davis, S.C. (2013). Interactions of Methicillin Resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in Polymicrobial Wound Infection. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0056846"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1080\/08927014.2013.878333","article-title":"Activity of disinfectants against multispecies biofilms formed by Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa","volume":"30","author":"Kart","year":"2014","journal-title":"Biofouling"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pbio.2003981","article-title":"Pseudomonas aeruginosa exoproducts determine antibiotic efficacy against Staphylococcus aureus","volume":"15","author":"Radlinski","year":"2017","journal-title":"PLoS Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6141","DOI":"10.1007\/s00253-016-7596-3","article-title":"Interactions between Pseudomonas aeruginosa and Staphylococcus aureus during co-cultivations and polymicrobial infections","volume":"100","author":"Nguyen","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"418","DOI":"10.3389\/fcimb.2017.00418","article-title":"Activity of Bacteriophages in Removing Biofilms of Pseudomonas aeruginosa Isolates from Chronic Rhinosinusitis Patients","volume":"7","author":"Fong","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"S66","DOI":"10.25011\/cim.v39i6.27504","article-title":"Lytic Activity of Various Phage Cocktails on Multidrug-Resistant Bacteria","volume":"39","author":"Ozkan","year":"2016","journal-title":"Clin. Investig. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.mib.2017.09.004","article-title":"Phage therapy as an alternative or complementary strategy to prevent and control biofilm-related infections","volume":"39","author":"Pires","year":"2017","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.resmic.2012.08.008","article-title":"Phage-antibiotic synergism: A possible approach to combatting Pseudomonas aeruginosa","volume":"164","author":"Knezevic","year":"2013","journal-title":"Res. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Torres-Barcel\u00f3, C., Arias-S\u00e1nchez, F.I., Vasse, M., Ramsayer, J., Kaltz, O., and Hochberg, M.E. (2014). A window of opportunity to control the bacterial pathogen Pseudomonas aeruginosa combining antibiotics and phages. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0106628"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.tim.2015.12.011","article-title":"Evolutionary Rationale for Phages as Complements of Antibiotics","volume":"24","author":"Hochberg","year":"2016","journal-title":"Trends Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Chaudhry, W.N., Concepcion-Acevedo, J., Park, T., Andleeb, S., Bull, J.J., and Levin, B.R. (2017). Synergy and order effects of antibiotics and phages in killing Pseudomonas aeruginosa biofilms. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0168615"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"28115","DOI":"10.1038\/srep28115","article-title":"A proposed integrated approach for the preclinical evaluation of phage therapy in Pseudomonas infections","volume":"6","author":"Vandenheuvel","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s00705-006-0849-1","article-title":"5500 Phages examined in the electron microscope","volume":"152","author":"Ackermann","year":"2007","journal-title":"Arch. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.virol.2015.04.008","article-title":"Prevalence of Pf1-like (pro)phage genetic elements among Pseudomonas aeruginosa isolates","volume":"483","author":"Knezevic","year":"2015","journal-title":"Virology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8079","DOI":"10.1128\/JB.01138-07","article-title":"Nutritional cues control Pseudomonas aeruginosa multicellular behavior in cystic fibrosis sputum","volume":"189","author":"Palmer","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"19890","DOI":"10.1073\/pnas.0606756104","article-title":"Selection for Staphylococcus aureus small-colony variants due to growth in the presence of Pseudomonas aeruginosa","volume":"103","author":"Hoffman","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2148","DOI":"10.1099\/mic.0.028001-0","article-title":"Pseudomonas aeruginosa extracellular products inhibit staphylococcal growth, and disrupt established biofilms produced by Staphylococcus epidermidis","volume":"155","author":"Qin","year":"2009","journal-title":"Microbiology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1111\/j.1365-2958.2006.05306.x","article-title":"The phenazine pyocyanin is a terminal signalling factor in the quorum sensing network of Pseudomonas aeruginosa","volume":"61","author":"Dietrich","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4718","DOI":"10.1128\/IAI.02198-14","article-title":"Synergistic Interactions of Pseudomonas aeruginosa and Staphylococcus aureus in an In Vitro Wound Model","volume":"82","author":"DeLeon","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.3389\/fmicb.2017.01229","article-title":"A Genotypic Analysis of Five P. aeruginosa Strains after Biofilm Infection by Phages Targeting Different Cell Surface Receptors","volume":"8","author":"Pires","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0130548","article-title":"Investigation of a Large Collection of Pseudomonas aeruginosa Bacteriophages Collected from a Single Environmental Source in Abidjan, C\u00f4te d\u2019Ivoire","volume":"10","author":"Essoh","year":"2015","journal-title":"PLoS ONE"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3295","DOI":"10.1099\/mic.0.2007\/007567-0","article-title":"Inhibition of bacteriophage replication in Streptococcus thermophilus by subunit poisoning of primase","volume":"153","author":"Sturino","year":"2007","journal-title":"Microbiology"},{"key":"ref_30","first-page":"fiy107","article-title":"Transient negative effects of antibiotics on phages do not jeopardise the advantages of combination therapies","volume":"94","author":"Gurney","year":"2018","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_31","first-page":"1315","article-title":"Role of the Pre-neck Appendage Protein (Dpo7) from Phage vB_SepiS-phiIPLA7 as an Anti-biofilm Agent in Staphylococcal Species","volume":"6","author":"Briers","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"162","DOI":"10.4292\/wjgpt.v8.i3.162","article-title":"Phage therapy: An alternative to antibiotics in the age of multi-drug resistance","volume":"8","author":"Lin","year":"2017","journal-title":"World J. Gastrointest. Pharmacol. Ther."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1080\/08927014.2015.1131821","article-title":"Discrimination of bacteriophage infected cells using locked nucleic acid fluorescent in situ hybridization (LNA-FISH)","volume":"32","author":"Almeida","year":"2016","journal-title":"Biofouling"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.tim.2013.12.004","article-title":"Interactions in multispecies biofilms: Do they actually matter?","volume":"22","author":"Ren","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/IAI.00116-17","article-title":"Albumin Inhibits Pseudomonas aeruginosa Quorum Sensing and Alters Polymicrobial Interactions","volume":"85","author":"Smith","year":"2017","journal-title":"Infect. Immun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1128\/JB.00059-15","article-title":"Coculture of Staphylococcus aureus with Pseudomonas aeruginosa Drives S. aureus towards Fermentative Metabolism and Reduced Viability in a Cystic Fibrosis Model","volume":"197","author":"Filkins","year":"2015","journal-title":"J. Bacteriol."},{"key":"ref_37","first-page":"26","article-title":"Diabetic Foot Infections: Current Diagnosis and Treatment","volume":"4","author":"Mendes","year":"2012","journal-title":"J. Diabet. Foot Complicat."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"7512","DOI":"10.4049\/jimmunol.175.11.7512","article-title":"The exopolysaccharide alginate protects Pseudomonas aeruginosa biofilm bacteria from IFN-gamma-mediated macrophage killing","volume":"175","author":"Leid","year":"2005","journal-title":"J. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.mib.2007.09.010","article-title":"Role of polysaccharides in Pseudomonas aeruginosa biofilm development","volume":"10","author":"Ryder","year":"2007","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1111\/j.1462-2920.2011.02657.x","article-title":"The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix","volume":"14","author":"Colvin","year":"2012","journal-title":"Environ. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"203","DOI":"10.3389\/fphar.2018.00203","article-title":"Interference in Bacterial Quorum Sensing: A Biopharmaceutical Perspective","volume":"9","author":"Mion","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"143564","DOI":"10.1155\/2014\/143564","article-title":"Toxic Anterior Segment Syndrome following Phacoemulsification Secondary to Overdose of Intracameral Gentamicin","volume":"2014","author":"Koban","year":"2014","journal-title":"Case Rep. Med."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Melo, L.D.R., Brand\u00e3o, A., Akturk, E., Santos, S.B., and Azeredo, J. (2018). Characterization of a new Staphylococcus aureus Kayvirus harboring a lysin active against biofilms. Viruses, 10.","DOI":"10.3390\/v10040182"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"10543","DOI":"10.1007\/s00253-016-7858-0","article-title":"Characterization and genome sequencing of a Citrobacter freundii phage CfP1 harboring a lysin active against multidrug-resistant isolates","volume":"100","author":"Oliveira","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Aziz, R.K., Bartels, D., Best, A., DeJongh, M., Disz, T., Edwards, R.A., Formsma, K., Gerdes, S., Glass, E.M., and Kubal, M. (2008). The RAST Server: Rapid annotations using subsystems technology. BMC Genom., 9.","DOI":"10.1186\/1471-2164-9-75"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"W686","DOI":"10.1093\/nar\/gki366","article-title":"The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs","volume":"33","author":"Schattner","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/nar\/gkh152","article-title":"ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences","volume":"32","author":"Laslett","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1093\/bioinformatics\/bti125","article-title":"Protein homology detection by HMM-HMM comparison","volume":"21","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.jmb.2004.05.028","article-title":"Improved prediction of signal peptides: SignalP 3.0","volume":"340","author":"Bendtsen","year":"2004","journal-title":"J. Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1093\/nar\/gkp335","article-title":"MEME Suite: Tools for motif discovery and searching","volume":"37","author":"Bailey","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"11","DOI":"10.4161\/rna.8.1.13346","article-title":"ARNold: A web tool for the prediction of rho-independent transcription terminators","volume":"8","author":"Naville","year":"2011","journal-title":"RNA Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkv487","article-title":"OrthoVenn: A web server for genome wide comparison and annotation of orthologous clusters across multiple species","volume":"43","author":"Wang","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4030","DOI":"10.1039\/C6FO01201A","article-title":"Co-loaded proteinase K\/thyme oil liposomes for inactivation of Escherichia coli O157:H7 biofilms on cucumber","volume":"7","author":"Cui","year":"2016","journal-title":"Food Funct."},{"key":"ref_55","unstructured":"Costa, S. (2016). Development of a Phage-Based Lab-on-Chip for the Detection of Foodborne Pathogens. [Master\u2019s Thesis, University of Minho]."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Cerca, N., Gomes, F., Pereira, S., Teixeira, P., and Oliveira, R. (2012). Confocal laser scanning microscopy analysis of S. epidermidis biofilms exposed to farnesol, vancomycin and rifampicin. BMC Res. Notes, 5.","DOI":"10.1186\/1756-0500-5-244"}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/8\/3\/103\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:09:32Z","timestamp":1760188172000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/8\/3\/103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,25]]},"references-count":56,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,9]]}},"alternative-id":["antibiotics8030103"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics8030103","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,25]]}}}