{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T02:25:22Z","timestamp":1791512722438,"version":"4.3.4"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,17]],"date-time":"2024-08-17T00:00:00Z","timestamp":1723852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Stavanger University Hospital, DailyColors\u2122"},{"name":"Muhdo Health Ltd."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>DailyColors\u2122 is a supplement made up of several phytonutrients that aims to replicate elements from the Mediterranean diet. These include fruit, berry and vegetable extracts that are rich in key phytochemicals such as Quercetin, Catechins, Phloretin, Ellagic Acid, and Anthocyanins. Here, we determined the effects of DailyColors\u2122 on the blood biomarkers associated with the diverse mechanisms implicated in ageing and age-related diseases, including mitochondrial function, inflammation, and oxidative stress, as well as on saliva\u2019s DNA methylation pattern. Thirty adult participants (mean (SD) age = 67.0 (7.5) years) with a body mass index over 25 were recruited into this randomised, double-blind, placebo-controlled, cross-over trial (two one-week treatment periods, separated by a one-week washout period). During the placebo period, we observed a significant increase in blood CD38 concentrations from the baseline to 24 h (p-value = 0.019). This was not observed in the active period. Increased CD38 is reportedly associated with subsequent mitochondrial dysfunction and inflammation. Next, there was a decreasing trend of plasma 4-HNE levels, an oxidative stress biomarker, after a one-week intake of DailyColors\u2122. Furthermore, following a one-month open-label follow-up in 26 participants, we observed hypermethylation of the candidate CpG site cg13108341 (q-value = 0.021), which was against the observed trend for this site during ageing. Taken together, while minimal effects were observed in this study, DailyColors\u2122 supplementation may be beneficial by altering and alleviating age-related changes. Longer and larger scale trials of DailyColors\u2122 supplementation are warranted.<\/jats:p>","DOI":"10.3390\/antiox13080995","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T10:11:28Z","timestamp":1724062288000},"page":"995","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A Randomised, Double-Blind, Placebo-Controlled, Cross-Over Clinical Trial to Evaluate the Biological Effects and Safety of a Polyphenol Supplement on Healthy Ageing"],"prefix":"10.3390","volume":"13","author":[{"given":"Joyce Ruifen","family":"Chong","sequence":"first","affiliation":[{"name":"Centre for Healthy Brain Ageing, Institute of Psychiatry, Psychology and Neuroscience, King\u2019s College London, London S5 9NU, UK"},{"name":"Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117600, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7541-2814","authenticated-orcid":false,"given":"Chiara","family":"de Lucia","sequence":"additional","affiliation":[{"name":"Centre for Healthy Brain Ageing, Institute of Psychiatry, Psychology and Neuroscience, King\u2019s College London, London S5 9NU, UK"},{"name":"Centre for Age-Related Medicine, Stavanger University Hospital, 4019 Stavanger, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego Alejandro","family":"Tovar-Rios","sequence":"additional","affiliation":[{"name":"Centre for Age-Related Medicine, Stavanger University Hospital, 4019 Stavanger, Norway"},{"name":"Grupos de Investigaci\u00f3n en Estad\u00edstica Aplicada\u2014INFERIR, Faculty of Engineering, Universidad del Valle, Santiago de Cali 760042, Colombia"},{"name":"Grupo Prevenci\u00f3n y Control de la Enfermedad Cr\u00f3nica\u2014PRECEC, Faculty of Health, Universidad del Valle, Santiago de Cali 760042, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-2611","authenticated-orcid":false,"given":"Nicolas","family":"Castellanos-Perilla","sequence":"additional","affiliation":[{"name":"Centre for Age-Related Medicine, Stavanger University Hospital, 4019 Stavanger, Norway"},{"name":"Department of Clinical Medicine, University of Bergen, 5009 Bergen, Norway"},{"name":"Semillero de Neurociencias y Envejecimiento, Ageing Institute, Medical School, Pontificia Universidad Javeriana, Bogot\u00e1 110231, Colombia"},{"name":"Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, 171 65 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2850-1950","authenticated-orcid":false,"given":"Christopher","family":"Collins","sequence":"additional","affiliation":[{"name":"Muhdo Health Ltd., Columba House, Adastral Park, Martlesham Heath, Martlesham, Ipswich IP5 3RE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silje Meihack","family":"Kvernberg","sequence":"additional","affiliation":[{"name":"Centre for Age-Related Medicine, Stavanger University Hospital, 4019 Stavanger, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clive","family":"Ballard","sequence":"additional","affiliation":[{"name":"University of Exeter Medical School, University of Exeter, Exeter EX1 2HZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4603-9763","authenticated-orcid":false,"given":"Richard C.","family":"Siow","sequence":"additional","affiliation":[{"name":"School of Cardiovascular and Metabolic Medicine & Sciences, British Heart Foundation Centre of Research Excellence, Faculty of Life Sciences & Medicine, King\u2019s College London, London SE1 9NH, UK"},{"name":"Ageing Research at King\u2019s (ARK), King\u2019s College London, London SE1 9NH, UK"},{"name":"Department of Physiology, Anatomy and Genetics, Medical Sciences Division, University of Oxford, Oxford OX1 3PT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dag","family":"Aarsland","sequence":"additional","affiliation":[{"name":"Centre for Healthy Brain Ageing, Institute of Psychiatry, Psychology and Neuroscience, King\u2019s College London, London S5 9NU, UK"},{"name":"Centre for Age-Related Medicine, Stavanger University Hospital, 4019 Stavanger, Norway"},{"name":"Ageing Research at King\u2019s (ARK), King\u2019s College London, London SE1 9NH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/S0140-6736(24)01296-0","article-title":"Dementia prevention, intervention, and care: 2024 report of the Lancet standing 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