{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T14:40:41Z","timestamp":1762353641079,"version":"build-2065373602"},"reference-count":78,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T00:00:00Z","timestamp":1741824000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT","award":["101084222"],"award-info":[{"award-number":["101084222"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["101084222"],"award-info":[{"award-number":["101084222"]}]},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB)","award":["101084222"],"award-info":[{"award-number":["101084222"]}]},{"name":"WASTELESS funding from HORIZONRIA","award":["101084222"],"award-info":[{"award-number":["101084222"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The European wine industry is embracing sustainability through circular economy principles, particularly by valorizing by-products, such as grape stems. Grape stems are rich in phenolic compounds with recognized health benefits. This study investigates the bioactive potential of molecules extracted from a blend of grape stems (GS blend extract). The GS blend extract was chemically characterized in terms of total phenolic content (TPC), ortho-diphenol content (ODC), and flavonoid content (FC), with key compounds identified via HPLC-MS. The extract\u2019s antioxidant capacity was assessed using ABTS, FRAP, and DPPH assays, while its anti-aging and depigmenting properties were evaluated through elastase and tyrosinase inhibition assays. Additionally, in vitro assays were conducted to assess its effects on skin cells, including morphology, metabolic activity, cell cycle, and cell migration. The GS blend extract was found to be rich in proanthocyanidins and exhibited notable antioxidant and depigmenting properties. In vitro assays demonstrated that the extract had no significant impact on cellular metabolic activity or cell morphology, although a reorganization of the cell monolayer was observed. Furthermore, deviations in cell migration and cell cycle regulation suggest that the GS blend extract may aid in scar formation management. Notably, the extract arrested fibroblasts in the Sub G0-G1 phase and inhibited HaCaT cell migration, supporting its potential application in cosmetic and pharmaceutical formulations aimed at scar modulation and skin health.<\/jats:p>","DOI":"10.3390\/antiox14030338","type":"journal-article","created":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T10:16:45Z","timestamp":1741861005000},"page":"338","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bioactive Potential of a Grape Stem Blend: A Sustainable Approach to Skin Regeneration"],"prefix":"10.3390","volume":"14","author":[{"given":"M\u00f3nica","family":"Serra","sequence":"first","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"},{"name":"Mesosystem Investiga\u00e7\u00e3o & Investimentos by Spinpark, Barco, 4805-017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8221-419X","authenticated-orcid":false,"given":"Claudia","family":"Botelho","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"},{"name":"Centre of Molecular and Environmental Biology (CBMA), Aquatic Research Network (ARNET), Associate Institute of Science and Innovation for Sustainability (IB-S), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2252-6666","authenticated-orcid":false,"given":"Diana","family":"Sousa","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"},{"name":"Mesosystem Investiga\u00e7\u00e3o & Investimentos by Spinpark, Barco, 4805-017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0799-2335","authenticated-orcid":false,"given":"Hugo","family":"Almeida","sequence":"additional","affiliation":[{"name":"Mesosystem Investiga\u00e7\u00e3o & Investimentos by Spinpark, Barco, 4805-017 Guimar\u00e3es, Portugal"},{"name":"UCIBIO, Laboratory of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, 4051-401 Porto, Portugal"},{"name":"Associate Laboratory i4HB Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4051-401 Porto, Portugal"}]},{"given":"Ana","family":"Casas","sequence":"additional","affiliation":[{"name":"Mesosystem Investiga\u00e7\u00e3o & Investimentos by Spinpark, Barco, 4805-017 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4918-3704","authenticated-orcid":false,"given":"Jos\u00e9 Ant\u00f3nio","family":"Teixeira","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"},{"name":"Centre of Molecular and Environmental Biology (CBMA), Aquatic Research Network (ARNET), Associate Institute of Science and Innovation for Sustainability (IB-S), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5834-6141","authenticated-orcid":false,"given":"Ana Novo","family":"Barros","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Argo-Environmental and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,13]]},"reference":[{"key":"ref_1","unstructured":"OIV\u2014International Organisation of Vine and Wine (2021). 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