{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T05:24:13Z","timestamp":1764221053727,"version":"3.46.0"},"reference-count":102,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T00:00:00Z","timestamp":1764028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa Carlos Chagas Filho do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/211.069\/2019","E-26\/211.651\/2021","E-26\/205.005\/2022"],"award-info":[{"award-number":["E-26\/211.069\/2019","E-26\/211.651\/2021","E-26\/205.005\/2022"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology","doi-asserted-by":"publisher","award":["CEECINST\/00060\/2021\/CP2902\/CT0004","2023.06590.CEECIND\/CP2852\/CT0002","2022.09196.PTDC"],"award-info":[{"award-number":["CEECINST\/00060\/2021\/CP2902\/CT0004","2023.06590.CEECIND\/CP2852\/CT0002","2022.09196.PTDC"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"BEAP-MAR project","award":["EAPA_0032\/2022"],"award-info":[{"award-number":["EAPA_0032\/2022"]}]},{"name":"Interreg Atlantic Area"},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Brazilian native seaweed Canistrocarpus cervicornis (Ochrophyta, Dictyotaceae) is recognized for its chemodiversity, particularly cyclic diterpenes and polysaccharides, yet its relevance to neurological disorders remains unexplored. This study evaluated the neuroprotective potential of a hydroethanolic extract (ECCH), its polar fraction (CCFPol), a dichloromethane extract (ECCD), and eight derived fractions (CCF1\u20132, CCF3, CCF4, CCF5\u20136, CCF7, CCF8\u201310, and CCF11\u201315). Cytotoxicity was evaluated in SH-SY5Y neuroblastoma cells, and neuroprotection was examined against 6-OHDA\u2013induced toxicity. The mitochondrial membrane potential, ROS and H2O2 generation assays were conducted to explore the mechanisms underlying the observed effects. Among the key findings, the CCF3 fraction exhibited a high content (75.04%) of dolastane-type diterpenoids. Both CCFPol (100 \u00b5g\/mL) and CCF3 (1 \u00b5g\/mL) increased cell viability to 68.43 \u00b1 4.60% and 60.61 \u00b1 0.80%, respectively, compared with 6-OHDA\u2013treated cells (50.70 \u00b1 2.71%). Additionally, CCF3 and CCFPol reduced H2O2 levels (200.0 \u00b1 18.19% and 195.5 \u00b1 16.13%, respectively, vs. 6-OHDA-treated cells: 302.2 \u00b1 17.07%) and lowered intracellular ROS (122.6 \u00b1 22.7% and 129.6 \u00b1 19.4%, respectively, vs. 6-OHDA-treated cells: 153.0 \u00b1 32.7%). This is the first study to demonstrate the neuroprotective potential of the C. cervicornis in a 6-OHDA-induced neurotoxicity cellular model, contributing to the understanding of marine bioactive resources and their relevance for neurological research. Additional studies aimed at isolating the active constituents and clarifying their mechanisms of action will further strengthen and expand the biological relevance of this specie as source of neuroprotective agents.<\/jats:p>","DOI":"10.3390\/antiox14121403","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T15:29:47Z","timestamp":1764084587000},"page":"1403","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Characterization and Bio-Screening of Neuroprotective Potential of Brazilian Brown Seaweed Canistrocarpus cervicornis in 6-OHDA-Induced Neurotoxicity Model"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2043-4805","authenticated-orcid":false,"given":"Thalisia Cunha","family":"dos Santos","sequence":"first","affiliation":[{"name":"Postgraduate Program in Chemistry of Natural Products, Instituto de Pesquisa em Produtos Naturais Walter Mors, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-599, Brazil"},{"name":"Seallg\u2014Solutions, Innovation, and Products, Startup, Bioeconomy Living Lab and Science and Technology-Based, Startup Incubators in the Ribeira Valley (Aqu\u00e1rio de Ideias), Registro 11900-000, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5351-2219","authenticated-orcid":false,"given":"Johana Marcela Concha","family":"Obando","sequence":"additional","affiliation":[{"name":"Seallg\u2014Solutions, Innovation, and Products, Startup, Bioeconomy Living Lab and Science and Technology-Based, Startup Incubators in the Ribeira Valley (Aqu\u00e1rio de Ideias), Registro 11900-000, Brazil"},{"name":"National Institute of Science and Technology (INCT) in Nanotechnology for Sustainable Agriculture (INCTNanoAgro), Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Sorocaba 18087-180, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1224-1699","authenticated-orcid":false,"given":"Joana","family":"Silva","sequence":"additional","affiliation":[{"name":"MARE-Marine and Environmental Sciences Centre & ARNET, ESTM, Polytechnic University of Leiria, 2520-630 Peniche, Portugal"}]},{"given":"Ana Lu\u00edza Vidal Pimentel","family":"Santos","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-853, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2325-7912","authenticated-orcid":false,"given":"Roberto Carlos Campos","family":"Martins","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Chemistry of Natural Products, Instituto de Pesquisa em Produtos Naturais Walter Mors, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-599, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6013-9889","authenticated-orcid":false,"given":"Diana Negr\u00e3o","family":"Cavalcanti","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Marine Biology and Coastal Ecosystems, Instituto de Biologia, Universidade Federal Fluminense, Niter\u00f3i 24210-201, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0970-0575","authenticated-orcid":false,"given":"Rui","family":"Pedrosa","sequence":"additional","affiliation":[{"name":"MARE-Marine and Environmental Sciences Centre & ARNET, ESTM, Polytechnic University of Leiria, 2520-630 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1581-2127","authenticated-orcid":false,"given":"Celso","family":"Alves","sequence":"additional","affiliation":[{"name":"MARE-Marine and Environmental Sciences Centre & ARNET, ESTM, Polytechnic University of Leiria, 2520-630 Peniche, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Nadeem, M.S., Hosawi, S., Alshehri, S., Ghoneim, M.M., Imam, S.S., Murtaza, B.N., and Kazmi, I. 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