{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T17:00:34Z","timestamp":1773248434497,"version":"3.50.1"},"reference-count":61,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T00:00:00Z","timestamp":1579478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The chestnut industry generates a large amount of by-products. These agro-industrial wastes have been described as potential sources of phenolic compounds with high bioactive potential. Therefore, we aimed to extract the phenolic compounds from chestnut by-products and assess their antioxidant potential and evaluate their antimicrobial activity against multidrug resistant bacteria. The individual phenolic compounds in the ethanolic extracts of chestnut shell, inner shell, bur, and leaves were characterized by HPLC-DAD\/electrospray ionization (ESI)-MS. The antioxidant properties were determined by DPPH and ABTS assays. The minimum inhibitory concentration (MIC) and the antimicrobial susceptibility was performed using the Kirby\u2013Bauer disc diffusion method against 10 bacterial strains. The major phenolic compounds identified in the extracts were trigalloyl-HHDP-glucose, gallic acid, quercetin, and myricetin glycoside derivatives. All chestnut by-products presented promising antioxidant activity in both assays, with leaf samples the ones presenting the highest antioxidant capacity. The inner shell\u2019s extract was effective against all Gram-positive and two Gram-negative bacteria; nevertheless, all extracts showed antibacterial activity. Staphylococcus epidermidis showed susceptibility to all extracts while none of the extracts was able to suppress the growth of Escherichia coli and Salmonella enteritidis. Chestnut by-products are a source of phenolic compounds with prominent antioxidant and antimicrobial activities. Nevertheless, further studies should be conducted to assess the correlation between phenolic compounds and the bioactivities obtained.<\/jats:p>","DOI":"10.3390\/antiox9010087","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T03:04:43Z","timestamp":1579575883000},"page":"87","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":86,"title":["Evaluation of the Phenolic Profile of Castanea sativa Mill. By-Products and Their Antioxidant and Antimicrobial Activity against Multiresistant Bacteria"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9406-8433","authenticated-orcid":false,"given":"Vanessa","family":"Silva","sequence":"first","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, 5000-801 Caparica, Portugal"}]},{"given":"Virg\u00edlio","family":"Falco","sequence":"additional","affiliation":[{"name":"Chemistry Research Centre (CQ-VR), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Maria In\u00eas","family":"Dias","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Braganc\u0327a, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9050-5189","authenticated-orcid":false,"given":"Lillian","family":"Barros","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Braganc\u0327a, 5300-253 Bragan\u00e7a, Portugal"}]},{"given":"Adriana","family":"Silva","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, 5000-801 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9584-0755","authenticated-orcid":false,"given":"Rosa","family":"Capita","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Veterinary Faculty, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"},{"name":"Institute of Food Science and Technology, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8688-0912","authenticated-orcid":false,"given":"Carlos","family":"Alonso-Calleja","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Veterinary Faculty, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"},{"name":"Institute of Food Science and Technology, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3648-7303","authenticated-orcid":false,"given":"Joana S.","family":"Amaral","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Braganc\u0327a, 5300-253 Bragan\u00e7a, Portugal"},{"name":"REQUIMTE-LAQV, Department of Chemical Sciences, Pharmacy Faculty, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6365-0735","authenticated-orcid":false,"given":"Gilberto","family":"Igrejas","sequence":"additional","affiliation":[{"name":"Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, 5000-801 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4910-4882","authenticated-orcid":false,"given":"Isabel","family":"C. F. R. Ferreira","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Braganc\u0327a, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-689X","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Poeta","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, 5000-801 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s00334-016-0575-x","article-title":"Unraveling the naturalness of sweet chestnut forests (Castanea sativa Mill.) in central Spain","volume":"26","author":"Glais","year":"2017","journal-title":"Veg. Hist. Archaeobot."},{"key":"ref_2","unstructured":"(2019, December 21). FAOSTAT. Available online: http:\/\/www.fao.org\/faostat\/en\/#home."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.wasman.2019.02.028","article-title":"Valorization of chestnut (Castanea sativa) residues: Characterization of different materials and optimization of the acid-hydrolysis of chestnut burrs for the elaboration of culture broths","volume":"87","author":"Paz","year":"2019","journal-title":"Waste Manag."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1080\/14786419.2017.1378199","article-title":"Recovery of bioactive molecules from chestnut (Castanea sativa Mill.) by-products through extraction by different solvents","volume":"32","author":"Vella","year":"2018","journal-title":"Nat. Prod. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.procbio.2017.09.017","article-title":"Chestnut (Castanea sativa Mill.) industrial wastes as a valued bioresource for the production of active ingredients","volume":"64","author":"Squillaci","year":"2018","journal-title":"Process. Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1080\/01496395.2013.838264","article-title":"Effect of the Extraction Technique and Operational Conditions on the Recovery of Bioactive Compounds from Chestnut (Castanea sativa) Bur and Shell","volume":"49","author":"Freire","year":"2014","journal-title":"Sep. Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.foodchem.2010.08.068","article-title":"Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic \u03b2-cells","volume":"125","year":"2011","journal-title":"Food Chem."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Esposito, T., Celano, R., Pane, C., Piccinelli, A.L., Sansone, F., Picerno, P., Zaccardelli, M., Aquino, R.P., and Mencherini, T. (2019). Chestnut (Castanea sativa Miller.) burs extracts and functional compounds: Uhplc-uv-hrms profiling, antioxidant activity, and inhibitory effects on phytopathogenic fungi. Molecules, 24.","DOI":"10.3390\/molecules24020302"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"T\u00fdskiewicz, K., Konkol, M., and R\u00f3j, E. (2018). The application of supercritical fluid extraction in phenolic compounds isolation from natural plant materials. Molecules, 23.","DOI":"10.3390\/molecules23102625"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Benincasa, C., Santoro, I., Nardi, M., Cassano, A., and Sindona, G. (2019). Eco-friendly extraction and characterisation of nutraceuticals from olive leaves. Molecules, 24.","DOI":"10.3390\/molecules24193481"},{"key":"ref_11","first-page":"1","article-title":"Synergistic antibacterial interaction between Melissa officinalis extracts and antibiotics Olgica Stefanovi \u0107 and Ljiljana Comic","volume":"2","author":"Comic","year":"2012","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6658","DOI":"10.1021\/jf300861c","article-title":"Evolution of Phenolic Compounds from Color and Flavor Problems to Health Benefits","volume":"60","author":"Gutierrez","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"81","DOI":"10.22159\/ajpcr.2016.v9i5.12173","article-title":"In vitro analysis of antimicrobial compounds from Alstonia scholaris","volume":"9","author":"Chakraborthy","year":"2016","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_14","first-page":"1567","article-title":"Biological Activities of Flavonoids: An overview","volume":"10","author":"Karak","year":"2019","journal-title":"Int. J. Pharm. Sci. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1590\/0034-737x201461000002","article-title":"Health promoting and sensory properties of phenolic compounds in food","volume":"61","author":"Melo","year":"2014","journal-title":"Rev. Ceres"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"270","DOI":"10.4161\/oxim.2.5.9498","article-title":"Plant polyphenols as dietary antioxidants in human health and disease","volume":"2","author":"Pandey","year":"2009","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_17","first-page":"1","article-title":"Bioactive Compounds in Medicinal Plants: A Condensed Review","volume":"1","author":"Takshak","year":"2018","journal-title":"SEJ Pharmacogn."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1080\/1040869059096","article-title":"Dietary Polyphenols and the Prevention of Diseases","volume":"45","author":"Scalbert","year":"2005","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"11161","DOI":"10.1021\/jf9033305","article-title":"Synthesis, Biological Evaluation, and Molecular Modeling of Oleuropein and Its Semisynthetic Derivatives as Cyclooxygenase Inhibitors","volume":"57","author":"Procopio","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.foodchem.2014.04.015","article-title":"Biomimetic synthesis and antioxidant evaluation of 3,4-DHPEA-EDA [2-(3,4-hydroxyphenyl) ethyl (3S,4E)-4-formyl-3-(2-oxoethyl)hex-4-enoate]","volume":"162","author":"Nardi","year":"2014","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.micpath.2019.03.025","article-title":"A review of the current evidence of fruit phenolic compounds as potential antimicrobials against pathogenic bacteria","volume":"130","author":"Lima","year":"2019","journal-title":"Microb. Pathog."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1001\/archinternmed.2010.79","article-title":"Nut Consumption and Blood Lipid Levels: A Pooled Analysis of 25 Intervention Trials","volume":"170","author":"Oda","year":"2010","journal-title":"JAMA Intern. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.jff.2015.06.018","article-title":"Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects\u2014A review","volume":"18","author":"Shahidi","year":"2015","journal-title":"J. Funct. Foods"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/j.foodchem.2007.09.030","article-title":"Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit","volume":"107","author":"Barreira","year":"2008","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00107-015-0936-7","article-title":"Antioxidant activity of phenolic extracts from chestnut fruit and forest industries residues","volume":"73","author":"Freire","year":"2015","journal-title":"Eur. J. Wood Wood Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.foodres.2013.07.064","article-title":"Effects of natural (grape seed and chestnut extract) and synthetic antioxidants (buthylatedhydroxytoluene, BHT) on the physical, chemical, microbiological and sensory characteristics of dry cured sausage \u201cchorizo\u201d","volume":"54","author":"Lorenzo","year":"2013","journal-title":"Food Res. Int."},{"key":"ref_27","first-page":"855","article-title":"Chestnut burs as a source of natural antioxidants","volume":"17","author":"Freire","year":"2009","journal-title":"Chem. Eng. Trans."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.indcrop.2008.03.003","article-title":"Antioxidant activity and phenolic content of chestnut (Castanea sativa) shell and eucalyptus (Eucalyptus globulus) bark extracts","volume":"28","author":"Fontenla","year":"2008","journal-title":"Ind. Crops Prod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"238","DOI":"10.21273\/JASHS.127.2.238","article-title":"Variability in Antioxidant Activity in Blueberry and Correlations among Different Antioxidant Activity Assays","volume":"127","author":"Connor","year":"2002","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/j.1755-0238.2006.tb00042.x","article-title":"Quantification of condensed tannins by precipitation with methyl cellulose: Development and validation of an optimised tool for grape and wine analysis","volume":"12","author":"Sarneckis","year":"2006","journal-title":"Aust. J. Grape Wine Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.indcrop.2016.04.065","article-title":"Phenolic profile and antioxidant activity of Coleostephus myconis (L.) Rchb.f.: An underexploited and highly disseminated species","volume":"89","author":"Bessada","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1248\/cpb.36.2090","article-title":"Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects","volume":"36","author":"Hatano","year":"1998","journal-title":"Chem. Pharm. Bull."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s10096-011-1303-1","article-title":"Detection and characterization of a ST6 clone of vanB2\u2014Enterococcus faecalis from three different hospitals in Spain","volume":"31","author":"Rezusta","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Jim\u00e9nez, E., Ladero, V., Chico, I., Maldonado-Barrag\u00e1n, A., L\u00f3pez, M., Mart\u00edn, V., Fern\u00e1ndez, L., Fern\u00e1ndez, M., \u00c1lvarez, M.A., and Torres, C. (2013). Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk. BMC Microbiol., 13.","DOI":"10.1186\/1471-2180-13-288"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1089\/fpd.2013.1689","article-title":"In Staphylococcus aureus of Meat Samples in Spain: Analysis of Immune Evasion Cluster (IEC) Genes","volume":"11","author":"Benito","year":"2014","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.eimc.2012.08.006","article-title":"Caracterizaci\u00f3n de cepas de Staphylococcus epidermidis y S. haemolyticus resistentes a meticilina y linezolid en un hospital espa nol","volume":"31","author":"Lozano","year":"2013","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1093\/jac\/dkr548","article-title":"qnr, aac(6\u2032)-Ib-cr and qepA genes in Escherichia coli and Klebsiella spp.: Genetic environments and plasmid and chromosomal location","volume":"67","author":"Ruiz","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2943","DOI":"10.1128\/AAC.00679-07","article-title":"Metallo-\u00df-Lactamase Gene blaIMP-15 in a Class 1 Integron, In 95, from Pseudomonas aeruginosa Clinical Isolates from a Hospital in Mexico","volume":"52","author":"Sader","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.foodcont.2018.05.031","article-title":"Chemical composition, antioxidant and antimicrobial activity of phenolic compounds extracted from wine industry by-products","volume":"92","author":"Silva","year":"2018","journal-title":"Food Control"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4166","DOI":"10.1016\/j.foodchem.2013.06.129","article-title":"The effect of feed moisture and temperature on tannin content, antioxidant and antimicrobial activities of extruded chestnuts","volume":"141","author":"Ryu","year":"2013","journal-title":"Food Chem."},{"key":"ref_42","first-page":"283","article-title":"Extraction and analysis of condensed tannins in Castanea sativa Mill","volume":"10","year":"2009","journal-title":"J. Cent. Eur. Agric."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.indcrop.2012.11.003","article-title":"Characterization of phenolic compounds in wild medicinal flowers from Portugal by HPLC\u2013DAD\u2013ESI\/MS and evaluation of antifungal properties","volume":"44","author":"Barros","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"232956","DOI":"10.1155\/2014\/232956","article-title":"Castanea sativa Mill. Flowers amongst the most powerful antioxidant matrices: A phytochemical approach in decoctions and infusions","volume":"2014","author":"Carocho","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.lwt.2015.11.017","article-title":"Characterization of phenolic composition in chestnut leaves and beer residue by LC-DAD-ESI-MS","volume":"68","author":"Munekata","year":"2016","journal-title":"LWT\u2014Food Sci. Technol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1080\/14786419.2017.1331225","article-title":"Phenolics from Castanea sativa leaves and their effects on UVB-induced damage","volume":"32","author":"Cerulli","year":"2018","journal-title":"Nat. Prod. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"9631","DOI":"10.1021\/jf102718t","article-title":"Phenolic Compounds in Chestnut (Castanea sativa Mill.) Heartwood. Effect of Toasting at Cooperage","volume":"58","author":"Sanz","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_48","first-page":"409","article-title":"Hydrolyzable Tannins from Sweet Chestnut Fractions Obtained by a Sustainable and Eco-friendly Industrial Process","volume":"11","author":"Campo","year":"2016","journal-title":"Nat. Prod. Commun."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Pycia, K., Kapusta, I., and Jaworska, G. (2019). Impact of the Degree of Maturity of Walnuts (Juglans regia L.) and Their Variety on the Antioxidant Potential and the Content of Tocopherols and Polyphenols. Molecules, 24.","DOI":"10.3390\/molecules24162936"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/14786419.2014.955488","article-title":"Castanea sativa by-products: A review on added value and sustainable application","volume":"29","author":"Braga","year":"2015","journal-title":"Nat. Prod. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.foodchem.2014.02.003","article-title":"Tannin analysis of chestnut bark samples (Castanea sativa Mill.) by HPLC-DAD\u2013MS","volume":"157","author":"Comandini","year":"2014","journal-title":"Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.foodchem.2013.11.158","article-title":"Comprehensive identification of walnut polyphenols by liquid chromatography coupled to linear ion trap\u2013Orbitrap mass spectrometry","volume":"152","author":"Regueiro","year":"2014","journal-title":"Food Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00049-008-0399-y","article-title":"Differential responses in American (Castanea dentata Marshall) and Chinese (C. mollissima Blume) chestnut (Falales: Fagaceae) to foliar application of jasmonic acid","volume":"18","author":"Cooper","year":"2008","journal-title":"Chemoecology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.indcrop.2011.06.022","article-title":"Response surface optimization of antioxidants extraction from chestnut (Castanea sativa) bur","volume":"35","author":"Freire","year":"2012","journal-title":"Ind. Crops Prod."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"108572","DOI":"10.1016\/j.foodres.2019.108572","article-title":"Nutritional, chemical and bioactive profiles of different parts of a Portuguese common fig (Ficus carica L.) variety","volume":"126","author":"Palmeira","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Martillanes, S., Rocha-Pimienta, J., Cabrera-Ba\u00f1egil, M., Mart\u00edn-Vertedor, D., and Delgado-Ad\u00e1mez, J. (2017). Application of Phenolic Compounds for Food Preservation: Food Additive and Active Packaging. Phenolic Compd. Biol. Act., 39\u201358.","DOI":"10.5772\/66885"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1021\/jf062614e","article-title":"Antimicrobial Activity of Tunisian Quince (Cydonia oblonga Miller) Pulp and Peel Polyphenolic Extracts","volume":"55","author":"Fattouch","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1016\/j.foodcont.2011.11.022","article-title":"p-Coumaric acid kills bacteria through dual damage mechanisms","volume":"25","author":"Lou","year":"2012","journal-title":"Food Control."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"61","DOI":"10.17221\/155\/2009-CJFS","article-title":"Scavenging capacity of superoxide radical and screening of antimicrobial activity of Castanea sativa mill. extracts","volume":"28","author":"Vidovic","year":"2010","journal-title":"Czech J. Food Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1055\/s-2007-967167","article-title":"Antioxidant, Antimalarial and Antimicrobial Activities of Tannin-Rich Fractions, Ellagitannins and Phenolic Acids from Punica granatum L.","volume":"73","author":"Reddy","year":"2007","journal-title":"Planta Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1016\/j.foodchem.2005.11.008","article-title":"Antimicrobial gallic acid from Caesalpinia mimosoides Lamk","volume":"100","author":"Chanwitheesuk","year":"2007","journal-title":"Food Chem."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/1\/87\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:44:08Z","timestamp":1760363048000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/1\/87"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,20]]},"references-count":61,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["antiox9010087"],"URL":"https:\/\/doi.org\/10.3390\/antiox9010087","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,20]]}}}