{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T15:41:16Z","timestamp":1772638876424,"version":"3.50.1"},"reference-count":58,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,11]],"date-time":"2020-07-11T00:00:00Z","timestamp":1594425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/MAR\/04292\/2020"],"award-info":[{"award-number":["UID\/MAR\/04292\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SAICTPAC\/0019\/2015 - LISBOA-01-0145-FEDER-016405)"],"award-info":[{"award-number":["SAICTPAC\/0019\/2015 - LISBOA-01-0145-FEDER-016405)"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["COMPETE (POCI-01-0145-FEDER-029250)"],"award-info":[{"award-number":["COMPETE (POCI-01-0145-FEDER-029250)"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund.","award":["Centro-01-0145-FEDER-000018"],"award-info":[{"award-number":["Centro-01-0145-FEDER-000018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Skin aging is a biological process influenced by intrinsic and extrinsic factors. The last ones, mainly exposure to UV radiation, increases reactive oxygen species (ROS) production leading to a loss of extracellular matrix, also enhanced by enzymatic degradation of matrix supporting molecules. Thus, and with the growing demand for eco-friendly skin products, natural compounds extracted from brown seaweeds revealed to be good candidates due to their broad range of bioactivities, especially as antioxidants. The aim of this study was to assess the dermo-cosmetic potential of different fractions obtained from the brown seaweed Fucus spiralis. For this purpose, in vitro antioxidant (Total Phenolic Content (TPC), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, Ferric Reducing Antioxidant Power (FRAP), Oxygen Radical Absorbance Capacity (ORAC)), anti-enzymatic (collagenase, elastase and hyaluronidase), antimicrobial, anti-inflammatory (NO production) and photoprotective (ROS production) capacities were evaluated. Although nearly all fractions evidenced antioxidant effects, fraction F10 demonstrated the highest antioxidant ability (EC50 of 38.5 \u00b5g\/mL, DPPH assay), and exhibited a strong effect as an inhibitor of collagenase (0.037 \u00b5g\/mL) and elastase (3.0 \u00b5g\/mL). Moreover, this fraction was also the most potent on reducing ROS production promoted by H2O2 (IC50 of 41.3 \u00b5g\/mL) and by UVB (IC50 of 31.3 \u00b5g\/mL). These bioactivities can be attributed to its high content of phlorotannins, as evaluated by LC-MS analysis, reinforcing the potential of F. spiralis for further dermatological applications.<\/jats:p>","DOI":"10.3390\/antiox9070611","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T04:46:01Z","timestamp":1594701961000},"page":"611","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Highlighting the Biological Potential of the Brown Seaweed Fucus spiralis for Skin Applications"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0194-0470","authenticated-orcid":false,"given":"Rafaela","family":"Freitas","sequence":"first","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"given":"Alice","family":"Martins","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"given":"Joana","family":"Silva","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1581-2127","authenticated-orcid":false,"given":"Celso","family":"Alves","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3290-3111","authenticated-orcid":false,"given":"Susete","family":"Pinteus","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0524-9270","authenticated-orcid":false,"given":"Joana","family":"Alves","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5384-1469","authenticated-orcid":false,"given":"Fernando","family":"Teodoro","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2429-7991","authenticated-orcid":false,"given":"Helena Margarida","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-038 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6799-2740","authenticated-orcid":false,"given":"L\u00eddia","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-038 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8104-7008","authenticated-orcid":false,"given":"\u017deljko","family":"Petrovski","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, REQUIMTE-CQFB, Faculdade de Ci\u00eancias e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Lu\u00eds","family":"Branco","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, REQUIMTE-CQFB, Faculdade de Ci\u00eancias e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Rui","family":"Pedrosa","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2520-641 Peniche, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1055\/a-0857-6633","article-title":"Current methods for the discovery of new active ingredients from natural products for cosmeceutical applications","volume":"85","year":"2019","journal-title":"Planta Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/07388551.2018.1503995","article-title":"Algal polysaccharides: Potential bioactive substances for cosmeceutical applications","volume":"39","author":"Fernando","year":"2018","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"308","DOI":"10.4161\/derm.22804","article-title":"Skin anti-aging strategies","volume":"4","author":"Ganceviciene","year":"2012","journal-title":"Dermato-Endocrinology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jdermsci.2010.03.003","article-title":"Role of antioxidants in the skin: Anti-aging effects","volume":"58","author":"Masaki","year":"2010","journal-title":"J. Dermatol. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"392","DOI":"10.3389\/fphar.2018.00392","article-title":"Natural antioxidants: Multiple mechanisms to protect skin from solar radiation","volume":"9","author":"Dunaway","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/761264","article-title":"Oxidative stress, prooxidants, and antioxidants: The interplay","volume":"2014","author":"Rahal","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7753","DOI":"10.3390\/ijms16047753","article-title":"Biological mechanisms underlying the ultraviolet radiation-induced formation of skin wrinkling and sagging I: Reduced skin elasticity, highly associated with enhanced dermal elastase activity, triggers wrinkling and sagging","volume":"16","author":"Imokawa","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"253","DOI":"10.4161\/derm.21923","article-title":"Hyaluronic acid: A key molecule in skin aging","volume":"4","author":"Papakonstantinou","year":"2012","journal-title":"Dermato-Endocrinology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"170170","DOI":"10.1016\/j.peptides.2019.170170","article-title":"Potential role of natural bioactive peptides for development of cosmeceutical skin products","volume":"122","author":"Liceaga","year":"2019","journal-title":"Peptides"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Pereira, L. (2018). Seaweeds as source of bioactive substances and skin care therapy\u2014Cosmeceuticals, algotheraphy, and thalassotherapy. Cosmetics, 5.","DOI":"10.3390\/cosmetics5040068"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Jesumani, V., Du, H., Aslam, M., Pei, P., and Huang, N. (2019). Potential use of seaweed bioactive compounds in skincare\u2014A review. Mar. Drugs, 17.","DOI":"10.3390\/md17120688"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/j.nbt.2013.03.006","article-title":"Sustainable production of biologically active molecules of marine based origin","volume":"30","author":"Murray","year":"2013","journal-title":"New Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"777","DOI":"10.3389\/fphar.2018.00777","article-title":"From marine origin to therapeutics: The antitumor potential of marine algae-derived compounds","volume":"9","author":"Alves","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.algal.2018.06.018","article-title":"Marine invasive macroalgae: Turning a real threat into a major opportunity - the biotechnological potential of Sargassum muticum and Asparagopsis armata","volume":"34","author":"Pinteus","year":"2018","journal-title":"Algal Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Silva, J., Alves, C., Freitas, R., Martins, A., Pinteus, S., Ribeiro, J., Gaspar, H., Alfonso, A., and Pedrosa, R. (2019). Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson\u2019s disease model. Mar. Drugs, 17.","DOI":"10.3390\/md17020085"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"136796","DOI":"10.1016\/j.scitotenv.2020.136796","article-title":"Medusa polyps adherence inhibition: A novel experimental model for antifouling assays","volume":"715","author":"Pinteus","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Salehi, B., Sharifi-Rad, J., Seca, A.M.L., Pinto, D.C.G.A., Michalak, I., Trincone, A., Mishra, A.P., Nigam, M., Zam, W., and Martins, N. (2019). Current trends on seaweeds: Looking at chemical composition, phytopharmacology, and cosmetic applications. Molecules, 24.","DOI":"10.3390\/molecules24224182"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Catarino, M.D., Silva, A.M.S., and Cardoso, S.M. (2018). Phycochemical Constituents and Biological Activities of Fucus spp.. Mar. Drugs, 16.","DOI":"10.3390\/md16080249"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"66","DOI":"10.3390\/cosmetics2020066","article-title":"Topical benefits of two fucoidan-rich extracts from marine macroalgae","volume":"2","author":"Fitton","year":"2015","journal-title":"Cosmetics"},{"key":"ref_20","first-page":"1","article-title":"Treatment of human skin with an extract of Fucus vesiculosus changes its thickness and mechanical properties","volume":"53","author":"Fujimura","year":"2002","journal-title":"J. Cosmet. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.foodchem.2016.09.067","article-title":"Cytoprotective effect of seaweeds with high antioxidant activity from the Peniche coast (Portugal)","volume":"218","author":"Pinteus","year":"2017","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a free radical method to evaluate antioxidant activity","volume":"28","author":"Cuvelier","year":"1995","journal-title":"LWT"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1021\/jf0305231","article-title":"Extending applicability of the Oxygen Radical Absorbance Capacity (ORAC\u2212Fluorescein) Assay","volume":"52","author":"Davalos","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The Ferric Reducing Ability of Plasma (FRAP) as a Measure of \u201cAntioxidant Power\u201d: The FRAP Assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_26","first-page":"1","article-title":"Evaluation of Trametes lactinea extracts on the inhibition of hyaluronidase, lipoxygenase and xanthine oxidase activities in vitro","volume":"23","author":"Yahaya","year":"2012","journal-title":"J. Phys. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0731-7085(93)80019-W","article-title":"Optimization of the USP assay for hyaluronidase","volume":"11","author":"Bailey","year":"1993","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"245","DOI":"10.2478\/v10102-009-0022-2","article-title":"Inhibition of nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages by Jeju plant extracts","volume":"2","author":"Yang","year":"2009","journal-title":"Interdiscip. Toxicol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Marto, J., Neves, \u00c2., Gon\u00e7alves, L.M., Pinto, P., Almeida, C.M.M., and Sim\u00f5es, S. (2018). Rice water: A traditional ingredient with anti-aging efficacy. Cosmetics, 5.","DOI":"10.3390\/cosmetics5020026"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3555","DOI":"10.1002\/rcm.3767","article-title":"A non-covalent dimer formed in electrospray ionisation mass spectrometry behaving as a precursor for fragmentations","volume":"22","author":"Pan","year":"2008","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.3390\/md10122766","article-title":"Phlorotannin extracts from Fucales characterized by HPLC-DAD-ESI-MSn: Approaches to hyaluronidase inhibitory capacity and antioxidant properties","volume":"10","author":"Ferreres","year":"2012","journal-title":"Mar. Drugs"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1007\/s11306-013-0584-z","article-title":"UPLC-MS profiling of low molecular weight phlorotannin polymers in Ascophyllum nodosum, Pelvetia canaliculata and Fucus spiralis","volume":"10","author":"Tierney","year":"2013","journal-title":"Metabolomics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.algal.2017.11.025","article-title":"Profiling phlorotannins from Fucus spp. of the Northern Portuguese coastline: Chemical approach by HPLC-DAD-ESI\/MS and UPLC-ESI-QTOF\/MS","volume":"29","author":"Lopes","year":"2018","journal-title":"Algal Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1016\/j.foodchem.2017.08.032","article-title":"Structure dependent antioxidant capacity of phlorotannins from Icelandic Fucus vesiculosus by UHPLC-DAD-ECD-QTOFMS","volume":"240","author":"Hermund","year":"2018","journal-title":"Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Catarino, M.D., Silva, A.M.S., Mateus, N., and Cardoso, S.M. (2019). Optimization of phlorotannins extraction from Fucus vesiculosus and evaluation of their potential to prevent metabolic disorders. Mar. Drugs, 17.","DOI":"10.3390\/md17030162"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Catarino, M.D., Silva, A.M.S., and Cardoso, S.M. (2017). Fucaceae: A source of bioactive phlorotannins. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18061327"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Paiva, L., Lima, E., Neto, A.I., and Baptista, J. (2018). Seasonal variability of the biochemical composition and antioxidant properties of Fucus spiralis at two Azorean islands. Mar. Drugs, 16.","DOI":"10.3390\/md16080248"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1515\/bot-2018-0047","article-title":"Pharmacological effects of Fucus spiralis extracts and phycochemicals: A comprehensive review","volume":"62","author":"Rosa","year":"2019","journal-title":"Bot. Mar."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1016\/j.procbio.2011.09.015","article-title":"Phlorotannins as bioactive agents from brown algae","volume":"46","author":"Li","year":"2011","journal-title":"Process. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Pimentel, F.B., Alves, R.C., Rodrigues, F., and Oliveira, M.B.P. (2017). Macroalgae-derived ingredients for cosmetic industry\u2014An update. Cosmetics, 5.","DOI":"10.3390\/cosmetics5010002"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1089\/jmf.2017.3992","article-title":"Saccharina japonica extract suppresses stemness of glioma stem cells by degrading epidermal growth factor receptor\/epidermal growth factor receptor variant III","volume":"21","author":"Kim","year":"2018","journal-title":"J. Med. Food"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ciko\u0161, A.-M., Jerkovi\u0107, I., Molnar, M., \u0160ubari\u0107, D., and Joki\u0107, S. (2019). New trends for macroalgal natural products applications. Nat. Prod. Res., 1\u201312.","DOI":"10.1080\/14786419.2019.1644629"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Kim, J.H., Lee, J.-E., Kim, K.H., and Kang, N.J. (2018). Beneficial effects of marine algae-derived carbohydrates for skin health. Mar. Drugs, 16.","DOI":"10.20944\/preprints201810.0772.v1"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/jdv.15043","article-title":"Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: A brief look at the latest updates","volume":"32","author":"Dreno","year":"2018","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1128\/CMR.00021-11","article-title":"The Malassezia Genus in Skin and Systemic Diseases","volume":"25","author":"Gaitanis","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1093\/mmy\/myx134","article-title":"Malassezia ecology, pathophysiology, and treatment","volume":"56","author":"Theelen","year":"2018","journal-title":"Med Mycol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Lopes, G., Sousa, C., Silva, L.R., Pinto, E., Andrade, P.B., Bernardo, J., Mouga, T., and Valent\u00e3o, P. (2012). Can phlorotannins purified extracts constitute a novel pharmacological alternative for microbial infections with associated inflammatory conditions?. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0031145"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Barbalace, M.C., Malaguti, M., Giusti, L., Lucacchini, A., Hrelia, S., and Angeloni, C. (2019). Anti-inflammatory activities of marine algae in neurodegenerative diseases. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20123061"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.3390\/md12031406","article-title":"Anti-inflammatory potential of monogalactosyl diacylglycerols and a monoacylglycerol from the edible brown seaweed Fucus spiralis Linnaeus","volume":"12","author":"Lopes","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_51","first-page":"2539","article-title":"Comparative study by GC-MS and chemometrics on the chemical and nutritional profile of Fucus spiralis L. juvenile and mature life-cycle phases","volume":"30","author":"Seca","year":"2018","journal-title":"Environ. Boil. Fishes"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Prasedya, E.S., Syafitri, S.M., Geraldine, B.A.F.D., Hamdin, C.D., Frediansyah, A., Miyake, M., Kobayashi, D., Hazama, A., and Sunarpi, H. (2019). UVA photoprotective activity of brown macroalgae Sargassum cristafolium. Biomedicines, 7.","DOI":"10.3390\/biomedicines7040077"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Pangestuti, R., Siahaan, E.A., and Kim, S.-K. (2018). Photoprotective substances derived from marine algae. Mar. Drugs, 16.","DOI":"10.3390\/md16110399"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Pozharitskaya, O.N., Shikov, A.N., Obluchinskaya, E.D., and Vuorela, H. (2019). The pharmacokinetics of fucoidan after topical application to rats. Mar. Drugs, 17.","DOI":"10.3390\/md17120687"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Pozharitskaya, O.N., Obluchinskaya, E.D., and Shikov, A.N. (2020). Mechanisms of bioactivities of fucoidan from the brown seaweed Fucus vesiculosus L. of the Barents Sea. Mar. Drugs, 18.","DOI":"10.3390\/md18050275"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s11094-015-1250-8","article-title":"Composition and anticoagulant properties of dry Fucus extract","volume":"49","author":"Obluchinsksya","year":"2015","journal-title":"Pharm. Chem. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1146\/annurev-food-032818-121401","article-title":"Source, extraction, characterization, and applications of novel antioxidants from seaweed","volume":"10","author":"Jacobsen","year":"2019","journal-title":"Annu. Rev. Food Sci. Technol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/j.jsps.2016.11.002","article-title":"LC-ESI-MS\/MS profiling of phenolics from Eleutherococcus spp. inflorescences, structure-activity relationship as antioxidants, inhibitors of hyaluronidase and acetylcholinesterase","volume":"25","author":"Olech","year":"2017","journal-title":"Saudi Pharm. J."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/7\/611\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:50:24Z","timestamp":1760176224000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/7\/611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,11]]},"references-count":58,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["antiox9070611"],"URL":"https:\/\/doi.org\/10.3390\/antiox9070611","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,11]]}}}