{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T23:11:19Z","timestamp":1776813079739,"version":"3.51.2"},"reference-count":37,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T00:00:00Z","timestamp":1596412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["032094"],"award-info":[{"award-number":["032094"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Phenylethyl isothiocyanate (PEITC) was reported as a useful antioxidant, anti-inflammatory, and chemopreventive agent. Due to technological and stability issues, it is necessary to be able to extract PEITC from its natural matrix (watercress) through sustainable and scalable methodologies. In this article, we explored, for the first time, the extractive capacity of aqueous micellar systems (AMSs) of two non-ionic surfactants. For this, we compared the AMSs with conventional organic solvents. Furthermore, we developed and optimised a new integral PEITC production and extraction process by a multifactorial experimental design. Finally, we analysed the antioxidant capacity by the oxygen radical absorbance capacity (ORAC) and ABTS methods. As results, the AMSs were able to extract PEITC at the same level as the tested conventional solvents. In addition, we optimised by response surface methodology the integrated process (2.0% m\/m, 25.0 \u00b0C, pH 9.0), which was equally effective (ca. 2900 \u00b5g PEITC\/g watercress), regardless of the surfactant used. The optimal extracts showed greater antioxidant capacity than pure PEITC, due to other antioxidant compounds extracted in the process. In conclusion, by the present work, we developed an innovative cost-effective and low environmental impact process for obtaining PEITC extracts from watercress by-products.<\/jats:p>","DOI":"10.3390\/antiox9080698","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T09:02:48Z","timestamp":1596445368000},"page":"698","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Phenylethyl Isothiocyanate Extracted from Watercress By-Products with Aqueous Micellar Systems: Development and Optimisation"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4496-2001","authenticated-orcid":false,"given":"Ezequiel R.","family":"Coscueta","sequence":"first","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0286-6639","authenticated-orcid":false,"given":"Celso A.","family":"Reis","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Institute of Molecular Pathology and Immunology of University of Porto, Ipatimup, 4200-135 Porto, Portugal"},{"name":"Medical Faculty, University of Porto, Al. Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"}]},{"given":"Manuela","family":"Pintado","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Voutsina, N., Payne, A.C., Hancock, R.D., Clarkson, G.J.J., Rothwell, S.D., Chapman, M.A., and Taylor, G. (2016). Characterization of the watercress (Nasturtium officinale R. Br.; Brassicaceae) transcriptome using RNASeq and identification of candidate genes for important phytonutrient traits linked to human health. BMC Genom., 17.","DOI":"10.1186\/s12864-016-2704-4"},{"key":"ref_2","first-page":"1","article-title":"Glucosinolates: Novel Sources and Biological Potential","volume":"Volume 1","author":"Montaut","year":"2015","journal-title":"Glucosinolates"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Romeo, L., Iori, R., Rollin, P., Bramanti, P., and Mazzon, E. (2018). Isothiocyanates: An Overview of Their Antimicrobial Activity against Human Infections. Molecules, 23.","DOI":"10.3390\/molecules23030624"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Mitsiogianni, M., Koutsidis, G., Mavroudis, N., Trafalis, D.T., Botaitis, S., Franco, R., Zoumpourlis, V., Amery, T., Galanis, A., and Pappa, A. (2019). The Role of Isothiocyanates as Cancer Chemo-Preventive, Chemo-Therapeutic and Anti-Melanoma Agents. Antioxidants, 8.","DOI":"10.3390\/antiox8040106"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2377","DOI":"10.1007\/s00394-018-1789-8","article-title":"Metabolic targets of watercress and PEITC in MCF-7 and MCF-10A cells explain differential sensitisation responses to ionising radiation","volume":"58","author":"Giallourou","year":"2019","journal-title":"Eur. J. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1007\/s10354-012-0163-3","article-title":"Sulforaphane and related mustard oils in focus of cancer prevention and therapy","volume":"163","author":"Herr","year":"2013","journal-title":"Wien. Med. Wochenschr."},{"key":"ref_7","first-page":"449","article-title":"Effects of Temperature and pH on Myrosinase Activity and Gluconasturtiin Hydrolysis Products in Watercress","volume":"3","author":"Farhana","year":"2016","journal-title":"Trans. Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.jfoodeng.2008.04.016","article-title":"Van Effects of pressure\/temperature treatments on stability and activity of endogenous broccoli (Brassica oleracea L. cv. Italica) myrosinase and on cell permeability","volume":"89","author":"Oey","year":"2008","journal-title":"J. Food Eng."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jfoodeng.2013.02.011","article-title":"Microwave-assisted extraction of sulforaphane from white cabbages: Effects of extraction condition, solvent and sample pretreatment","volume":"117","author":"Tanongkankit","year":"2013","journal-title":"J. Food Eng."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1039\/C8AY02442A","article-title":"Simplified analytical methodology for glucosinolate hydrolysis products: A miniaturized extraction technique and multivariate optimization","volume":"11","author":"Fusari","year":"2019","journal-title":"Anal. Methods"},{"key":"ref_11","unstructured":"Pusateri, D.J., Kizer, T.R., and Lowry, A.N. (2004). Extraction of Non-Polar Isothiocyanates from Plants. (6,824,796), US Patent."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"30905","DOI":"10.1039\/C5RA28068K","article-title":"Recovery of antioxidant and antiproliferative compounds from watercress using pressurized fluid extraction","volume":"6","author":"Rodrigues","year":"2016","journal-title":"RSC Adv."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Pereira, L., Silva, P., Duarte, M., Rodrigues, L., Duarte, C., Albuquerque, C., and Serra, A. (2017). Targeting Colorectal Cancer Proliferation, Stemness and Metastatic Potential Using Brassicaceae Extracts Enriched in Isothiocyanates: A 3D Cell Model-Based Study. Nutrients, 9.","DOI":"10.3390\/nu9040368"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.talanta.2017.02.065","article-title":"Evaluation of new natural deep eutectic solvents for the extraction of isoflavones from soy products","volume":"168","author":"Bajkacz","year":"2017","journal-title":"Talanta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1080\/00268970110068750","article-title":"Light scattering and fluorescence probe studies on micellar properties of Triton X-100 in KCl solutions","volume":"99","author":"Aguiar","year":"2001","journal-title":"Mol. Phys."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"875","DOI":"10.2116\/analsci.14.875","article-title":"Aqueous Micellar Two-Phase Systems for Protein Separation","volume":"14","author":"Tani","year":"1998","journal-title":"Anal. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.seppur.2016.07.052","article-title":"Single-step extraction of carotenoids from brown macroalgae using non-ionic surfactants","volume":"172","author":"Vieira","year":"2017","journal-title":"Sep. Purif. Technol."},{"key":"ref_18","first-page":"1122","article-title":"Application of cloud point extraction using surfactants in the isolation of physical antioxidants (phenols) from olive mill wastewater","volume":"15","author":"Katsoyannos","year":"2006","journal-title":"Proc. Fresenius Environ. Bull."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.foodchem.2016.07.001","article-title":"Integrated extraction and purification of soy isoflavones by using aqueous micellar systems","volume":"213","author":"Cordisco","year":"2016","journal-title":"Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"333","DOI":"10.3139\/113.110315","article-title":"Cloud point extraction of polycyclic aromatic hydrocarbons in aqueous solution with nonionic surfactants","volume":"51","author":"Alibrahim","year":"2014","journal-title":"Tenside Surfactants Deterg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s11746-007-1180-z","article-title":"Recovery of natural antioxidants from olive mill wastewater using Genapol-X080","volume":"85","author":"Gortzi","year":"2008","journal-title":"JAOCS J. Am. Oil Chem. Soc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7","DOI":"10.5923\/j.scit.20110101.02","article-title":"Aqueous Two Phase Systems for the Recovery of Biomolecules\u2014A Review","volume":"1","author":"Raja","year":"2012","journal-title":"Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.fluid.2015.03.011","article-title":"Physicochemical characterization of aqueous micellar systems formed by environmentally friendly salts","volume":"393","author":"Cordisco","year":"2015","journal-title":"Fluid Phase Equilib."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.foodchem.2015.03.106","article-title":"Surfactant mediated extraction of total phenolic contents (TPC) and antioxidants from fruits juices","volume":"185","author":"Sharma","year":"2015","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.foodchem.2018.05.015","article-title":"Micellar systems of aliphatic alcohol ethoxylates as a sustainable alternative to extract soybean isoflavones","volume":"264","author":"Coscueta","year":"2018","journal-title":"Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.lwt.2018.04.025","article-title":"Aqueous micellar two-phase system as an alternative method to selectively remove soy antinutritional factors","volume":"93","author":"Haidar","year":"2018","journal-title":"LWT"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"100006","DOI":"10.1016\/j.fochx.2019.100006","article-title":"Enzymatic soy protein hydrolysis: A tool for biofunctional food ingredient production","volume":"1","author":"Coscueta","year":"2019","journal-title":"Food Chem. X"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1021\/jf8020199","article-title":"Effects of Elevated CO2 on Grapevine (Vitis vinifera L.): Volatile Composition, Phenolic Content, and in vitro Antioxidant Activity of Red Wine","volume":"57","author":"Falco","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","unstructured":"Kutner, M.H., Nachtsheim, C.J., Neter, J., and Li, W. (2005). Applied Linear Statistical Models, McGraw Hill. [5th ed.]."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"99","DOI":"10.2307\/3001913","article-title":"Comparing individual means in the analysis of variance","volume":"5","author":"Tukey","year":"1949","journal-title":"Biometrics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"285","DOI":"10.2307\/1270613","article-title":"Response Surface Methodology: Process and Product Optimization Using Designed Experiments","volume":"38","author":"Gunst","year":"1996","journal-title":"Technometrics"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Grumezescu, A.M., and Holban, A.M. (2017). Ingredients Extraction by Physicochemical Methods in Food. Handbook of Food Bioengineering, Elsevier Science.","DOI":"10.1016\/B978-0-12-811521-3.00022-3"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"84","DOI":"10.3109\/10715762.2015.1108520","article-title":"Phenethyl isothiocyanate, by virtue of its antioxidant activity, inhibits invasiveness and metastatic potential of breast cancer cells: HIF-1\u03b1 as a putative target","volume":"50","author":"Sarkar","year":"2016","journal-title":"Free Radic. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1002\/em.20468","article-title":"Antioxidant and pro-oxidant capacities of ITCs","volume":"50","author":"Valgimigli","year":"2009","journal-title":"Environ. Mol. Mutagen."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/s00204-005-0656-6","article-title":"Chemoprevention of acrylamide toxicity by antioxidative agents in rats\u2014Effective suppression of testicular toxicity by phenylethyl isothiocyanate","volume":"79","author":"Lee","year":"2005","journal-title":"Arch. Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Soundararajan, P., and Kim, J. (2018). Anti-Carcinogenic Glucosinolates in Cruciferous Vegetables and Their Antagonistic Effects on Prevention of Cancers. Molecules, 23.","DOI":"10.3390\/molecules23112983"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/j.1439-0396.2005.00523.x","article-title":"The time-dependent effect of gluconasturtiin and phenethyl isothiocyanate on metabolic and antioxidative parameters in rats","volume":"89","author":"Okulicz","year":"2005","journal-title":"J. Anim. Physiol. Anim. Nutr. (Berl.)"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/8\/698\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:54:01Z","timestamp":1760176441000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/8\/698"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,3]]},"references-count":37,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8]]}},"alternative-id":["antiox9080698"],"URL":"https:\/\/doi.org\/10.3390\/antiox9080698","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,3]]}}}