{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T22:45:13Z","timestamp":1775601913136,"version":"3.50.1"},"reference-count":52,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Every year, large quantities of stems and pits are generated during sweet cherry processing, without any substantial use. Although stems are widely recognized by traditional medicine, detailed and feasible information about their bioactive composition or biological value is still scarce, as well as the characterization of kernels. Therefore, we conducted a study in which bioactivity potential of extracts from stems and kernels of four sweet cherry cultivars (Early Bigi (grown under net cover (C) and without net cover (NC)), Burlat, Lapins, and Van) were examined. The assays included antioxidant (by 2,2\u2032-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and \u03b2-carotene-linoleic acid bleaching assays), and antibacterial activities against important Gram negative and Gram positive bacterial human isolates. Profile and individual phenolic composition of each extract were determined by High-performance liquid chromatography (HPLC) analysis. Extracts from stems of cv. Lapins and kernels of Early Bigi NC presented high levels of total phenolics, flavonoids, ortho-diphenols and saponins. Excepting for cv. Early Bigi NC, major phenolic compounds identified in stems and kernels were sakuranetin and catechin, respectively. In cv. Early Bigi NC the most abundant compounds were ellagic acid for stems and protocatechuic acid for kernels. In all extracts, antioxidant activities showed a positive correlation with the increments in phenolic compounds. Antimicrobial activity assays showed that only stem\u2019s extracts were capable of inhibiting the growth of Gram positive isolates. This new data is intended to provide new possibilities of valorization of these by-products and their valuable properties.<\/jats:p>","DOI":"10.3390\/antiox9121295","type":"journal-article","created":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T10:42:47Z","timestamp":1608201767000},"page":"1295","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit"],"prefix":"10.3390","volume":"9","author":[{"given":"S\u00edlvia","family":"Afonso","sequence":"first","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences\u2014CITAB, University of Tr\u00e1s-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal"}]},{"given":"Ivo Vaz","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences\u2014CITAB, University of Tr\u00e1s-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal"}]},{"given":"Anne S.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine, Technical University of Denmark, DTU Building 221, DK-2800 Kgs. Lyngby, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6291-8413","authenticated-orcid":false,"given":"Alfredo","family":"Aires","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences\u2014CITAB, University of Tr\u00e1s-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7492-4965","authenticated-orcid":false,"given":"Maria Jos\u00e9","family":"Saavedra","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences\u2014CITAB, University of Tr\u00e1s-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5764-024X","authenticated-orcid":false,"given":"Berta","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences\u2014CITAB, University of Tr\u00e1s-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1002\/jsfa.8106","article-title":"Characterization of autochthonous sweet cherry cultivars (Prunus avium L.) of southern Italy for fruit quality, bioactive compounds and antioxidant activity","volume":"97","author":"Russo","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.foodchem.2007.08.004","article-title":"Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.)","volume":"107","author":"Usenik","year":"2008","journal-title":"Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/j.foodchem.2012.11.024","article-title":"Fruit quality and bioactive compounds relevant to human health of sweet cherry (Prunus avium L.) cultivars grown in Italy","volume":"140","author":"Ballistreri","year":"2013","journal-title":"Food Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.foodres.2014.06.020","article-title":"Chemical composition, nutritional value and antioxidant properties of autochthonous Prunus avium cultivars from Campania Region","volume":"64","author":"Pacifico","year":"2014","journal-title":"Food Res. Int."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.tifs.2019.02.052","article-title":"Sweet and sour cherries: Origin, distribution, nutritional composition and health benefits","volume":"86","author":"Blando","year":"2019","journal-title":"Trends Food Sci. Technol."},{"key":"ref_6","unstructured":"FAOSTAT (2018, July 06). Data. Available online: http:\/\/www.fao.org\/faostat\/en\/#data\/QC."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1002\/jctb.5532","article-title":"Bioactive compounds of sweet and sour cherry stems obtained by subcritical water extraction","volume":"93","author":"Clavijo","year":"2018","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.foodres.2018.08.079","article-title":"Exploring the phenolic profile, antioxidant, antidiabetic and anti-hemolytic potential of Prunus avium vegetal parts","volume":"116","author":"Jesus","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"265","DOI":"10.18388\/abp.2016_1374","article-title":"Analysis of glycosylated flavonoids extracted from sweet-cherry stems, as antibacterial agents against pathogenic Escherichia coli isolates","volume":"64","author":"Aires","year":"2017","journal-title":"Acta Biochim. Pol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1016\/j.foodchem.2014.10.145","article-title":"Chemical characterisation and bioactive properties of Prunus avium L.: The widely studied fruits and the unexplored stems","volume":"173","author":"Bastos","year":"2015","journal-title":"Food Chem."},{"key":"ref_11","first-page":"FCT73","article-title":"Anthocyanin and polyphenolic composition of fresh and processed cherries","volume":"69","author":"Chaovanalikit","year":"2004","journal-title":"J. Food Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1007\/s11130-016-0538-5","article-title":"Impact of cultivar on profile and concentration of lipophilic bioactive compounds in kernel oils recovered from sweet cherry (Prunus avium L.) by-products","volume":"71","author":"Raczyk","year":"2016","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3010","DOI":"10.1021\/jf0115589","article-title":"Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity","volume":"50","author":"Dewanto","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1002\/jsfa.5958","article-title":"A simple method for the determination of bioactive antioxidants in virgin olive oils","volume":"93","author":"Garcia","year":"2013","journal-title":"J. Sci. Food Agric."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.jfoodeng.2006.10.018","article-title":"Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum","volume":"81","author":"Chen","year":"2007","journal-title":"J. Food Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1042\/bst0110591","article-title":"Determination of total carotenoids and chlorophylls a and b of leaves in different solvents","volume":"11","author":"Lichtenthaler","year":"1983","journal-title":"Biochem. Soc. Trans."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1080\/01904160802135092","article-title":"Rapid measurement of chlorophylls with a microplate reader","volume":"31","author":"Warren","year":"2008","journal-title":"J. Plant Nutr."},{"key":"ref_19","first-page":"52","article-title":"Spectrophotometric determinations of chloroplastidic pigments in acetone, ethanol and dimethylsulphoxide","volume":"11","author":"Pompelli","year":"2013","journal-title":"Rev. Bras. Bioci\u00eanc."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2144","DOI":"10.1021\/jf020444+","article-title":"Antioxidant properties of various solvents extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam) leaves","volume":"51","author":"Siddhraju","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1021\/jf052334j","article-title":"Determination of total content of phenolic compounds and their antioxidant activity in vegetables evaluation of spectrophotometric methods","volume":"54","author":"Stratil","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_23","first-page":"1659","article-title":"Chemical compositions, antioxidant and antimicrobial activity of the essential oils of Piper officinarum (Piperaceae)","volume":"7","author":"Salleh","year":"2012","journal-title":"Nat. Prod. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1080\/19476337.2013.769025","article-title":"Phytochemical characterization and antioxidant properties of organic baby-leaf watercress produced under organic production system","volume":"11","author":"Aires","year":"2013","journal-title":"CYTA J. Food."},{"key":"ref_25","unstructured":"Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C., and Yolken, R. (2015). Antibacterial susceptibility tests: Dilution and disk diffusion methods. Manual of Clinical Microbiology, ASM Press. [7th ed.]."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2086","DOI":"10.1111\/j.1365-2672.2009.04180.x","article-title":"The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract","volume":"106","author":"Aires","year":"2009","journal-title":"J. Appl. Microbiol."},{"key":"ref_27","first-page":"1","article-title":"Phenolic compounds, antioxidant and antimicrobial properties of the wild cherry (Prunus avium L.) stem","volume":"48","year":"2017","journal-title":"Acta Period. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3471","DOI":"10.1039\/C9FO02693B","article-title":"Cherry stem infusions: Antioxidant potential and phenolic profile by UHPLC-ESI-QTOF-MS","volume":"11","author":"Peixoto","year":"2020","journal-title":"Food Funct."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.jep.2004.05.016","article-title":"Biological activities and distribution of plant saponins","volume":"94","author":"Sparg","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0147-9571(99)00020-X","article-title":"Isolation and evaluation of immunological adjuvant activities of saponins from Polygala senega L.","volume":"23","author":"Estrada","year":"2000","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.2174\/0929867023369709","article-title":"Traditional Chinese medicine (TCM): Are polyphenols and saponins the key ingredients triggering biological activities?","volume":"9","author":"Liu","year":"2002","journal-title":"Curr. Med. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"109584","DOI":"10.1016\/j.foodres.2020.109584","article-title":"Physiological genetics, chemical composition, health benefits and toxicology of tea (Camellia sinensis L.) flower: A review","volume":"137","author":"Chen","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4290","DOI":"10.1021\/jf0502698","article-title":"Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements","volume":"53","author":"Prior","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3396","DOI":"10.1021\/jf9913458","article-title":"Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing\/antioxidant power assay","volume":"48","author":"Pulido","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3122","DOI":"10.1021\/jf0116606","article-title":"Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: A comparative study","volume":"50","author":"Ou","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5126","DOI":"10.3390\/molecules17055126","article-title":"Comparison of the simple cyclic voltammetry (CV) and DPPH assays for the determination of antioxidant capacity of active principles","volume":"17","author":"Arteaga","year":"2012","journal-title":"Molecules"},{"key":"ref_37","first-page":"117","article-title":"A simple electrochemical method for the rapid estimation of antioxidant potentials of some selected medicinal plants","volume":"11","author":"Amidi","year":"2012","journal-title":"Iran. J. Pharm. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"152","DOI":"10.17660\/eJHS.2019\/84.3.5","article-title":"Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: Correlation with spectrophotometric assays","volume":"84","year":"2019","journal-title":"Eur. J. Hortic. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1600475","DOI":"10.1002\/mnfr.201600475","article-title":"Buckwheat bioactive compounds, their derived phenolic metabolites and their health benefits","volume":"61","author":"Zielinski","year":"2017","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1002\/jsfa.9209","article-title":"Effect of detoxification on biological quality of wild apricot (Prunus armeniaca L.) kernel","volume":"99","author":"Tanwar","year":"2019","journal-title":"J. Sci. Food Agric."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"10621","DOI":"10.1021\/acs.jafc.7b03408","article-title":"Fruit seeds of the Rosaceae family: A waste, new life, or a danger to human health?","volume":"65","author":"Senica","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.foodchem.2016.02.110","article-title":"Transition of phenolics and cyanogenic glycosides from apricot and cherry fruit kernels into liqueur","volume":"203","author":"Senica","year":"2016","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1590\/S0100-879X2009000400006","article-title":"Antioxidant and antimicrobial activities of bitter and sweet apricot (Prunus armeniaca L.) kernels","volume":"42","author":"Mavi","year":"2009","journal-title":"Braz. J. Med. Biol. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1007\/s11130-011-0246-0","article-title":"Antioxidant capacity and phenolics content of apricot (Prunus armeniaca L.) kernel as a function of genotype","volume":"66","author":"Korekar","year":"2011","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"162750","DOI":"10.1155\/2013\/162750","article-title":"Chemistry and biological activities of flavonoids: An overview","volume":"2013","author":"Kumar","year":"2013","journal-title":"Sci. World J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4606","DOI":"10.1128\/AEM.65.10.4606-4610.1999","article-title":"Mechanisms of action of carvacrol on the food-borne pathogen Bacillus cereus","volume":"65","author":"Ultee","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1111\/j.1750-3841.2011.02213.x","article-title":"Antibacterial activity and mechanism of action of chlorogenic acid","volume":"76","author":"Lou","year":"2011","journal-title":"J. Food Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1021\/jf203259h","article-title":"Anti-inflammatory and antifibrotic effects of naringenin in diabetic mice","volume":"60","author":"Tsai","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1016\/j.jnutbio.2013.05.001","article-title":"Benefits of polyphenols on gut microbiota and implications in human health","volume":"24","author":"Cardona","year":"2013","journal-title":"J. Nutr. Biochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1080\/13880209.2016.1230636","article-title":"Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs","volume":"55","author":"Nunes","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Taofiq, O., Gonz\u00e1lez-Param\u00e1s, A.M., Barreiro, M.F., and Ferreira, I.C. (2017). Hydroxycinnamic acids and their derivatives: Cosmeceutical significance, challenges and future perspectives, a review. Molecules, 22.","DOI":"10.3390\/molecules22020281"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.foodres.2014.01.057","article-title":"Extraction and quantification of saponins: A review","volume":"59","author":"Cheok","year":"2014","journal-title":"Food Res. Int."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/12\/1295\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:46:21Z","timestamp":1760179581000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/12\/1295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,17]]},"references-count":52,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["antiox9121295"],"URL":"https:\/\/doi.org\/10.3390\/antiox9121295","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,17]]}}}