{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T12:57:08Z","timestamp":1768568228347,"version":"3.49.0"},"reference-count":50,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T00:00:00Z","timestamp":1578009600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/ECM\/118372\/2010"],"award-info":[{"award-number":["PTDC\/ECM\/118372\/2010"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>This study applies a methodology to evaluate the ecotoxicological potential of raw materials and cement-based construction materials. In this study, natural aggregates and Portland cement were replaced with non-conventional recycled concrete aggregates (RA) and fly ash (FA), respectively, in the production of two concrete products alternative to conventional concrete (used as reference). The experimental program involved assessing both the chemical properties (non-metallic and metallic parameters) and ecotoxicity data (battery of tests with the luminescent bacterium Vibrio fischeri, the freshwater crustacean Daphnia magna, and the yeast Saccharomyces cerevisiae) of eluates obtained from leaching tests of RA, FA, and the three concrete mixes. Even though the results indicated that RA and FA have the ability to release some chemicals into the water and induce its alkalinisation, the respective eluate samples presented no or low levels of potential ecotoxicity. However, eluates from concrete mixes produced with a replacement ratio of Portland cement with 60% of FA and 100% of natural aggregates and produced with 60% of FA and 100% of RA were classified as clearly ecotoxic mainly towards Daphnia magna mobility. Therefore, raw materials with weak evidences of ecotoxicity could lead to the production of concrete products with high ecotoxicological potential. Overall, the results obtained highlight the importance of integrating data from the chemical and ecotoxicological characterization of materials\u2019 eluate samples aiming to assess the possible environmental risk of the construction materials, namely of incorporating non-conventional raw materials in concrete, and contributing to achieve construction sustainability.<\/jats:p>","DOI":"10.3390\/app10010351","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T04:43:03Z","timestamp":1578026583000},"page":"351","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Evaluation of the Ecotoxicological Potential of Fly Ash and Recycled Concrete Aggregates Use in Concrete"],"prefix":"10.3390","volume":"10","author":[{"given":"Patr\u00edcia","family":"Rodrigues","sequence":"first","affiliation":[{"name":"CERIS, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3710-9603","authenticated-orcid":false,"given":"Jos\u00e9 D.","family":"Silvestre","sequence":"additional","affiliation":[{"name":"CERIS, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4038-6748","authenticated-orcid":false,"given":"In\u00eas","family":"Flores-Colen","sequence":"additional","affiliation":[{"name":"CERIS, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1394-0731","authenticated-orcid":false,"given":"Cristina A.","family":"Viegas","sequence":"additional","affiliation":[{"name":"Bioengineering Department and iBB-Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2007-9257","authenticated-orcid":false,"given":"Hawreen H.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"CERIS, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"},{"name":"Department of Civil Engineering, Technical Engineering College, Erbil Polytechnic University, Kirkuk Road, Erbil 44001, Kurdistan Region, Iraq"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2429-4755","authenticated-orcid":false,"given":"Rawaz","family":"Kurda","sequence":"additional","affiliation":[{"name":"CERIS, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"},{"name":"Department of Civil Engineering, Technical Engineering College, Erbil Polytechnic University, Kirkuk Road, Erbil 44001, Kurdistan Region, Iraq"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6766-2736","authenticated-orcid":false,"given":"Jorge","family":"de Brito","sequence":"additional","affiliation":[{"name":"CERIS, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109258","DOI":"10.1016\/j.engstruct.2019.05.099","article-title":"Performance of fly ash incorporated recycled aggregates concrete column under axial compression: Experimental and numerical study","volume":"196","author":"Sunayana","year":"2019","journal-title":"Eng. Struct."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.jclepro.2017.07.236","article-title":"Effect of incorporation of high volume of recycled concrete aggregates and fly ash on the strength and global warming potential of concrete","volume":"166","author":"Kurad","year":"2017","journal-title":"J. Clean. Prod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.cemconcomp.2018.10.004","article-title":"Water absorption and electrical resistivity of concrete with recycled concrete aggregates and fly ash","volume":"95","author":"Kurda","year":"2019","journal-title":"Cem. Concr. Compos."},{"key":"ref_4","first-page":"1","article-title":"Effect of recycled concrete aggregate replacement level on the fracture behavior of concrete","volume":"3","author":"Arezoumandi","year":"2015","journal-title":"ACI Mater. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jcou.2018.11.004","article-title":"Carbonation of concrete made with high amount of fly ash and recycled concrete aggregates for utilization of CO2","volume":"29","author":"Kurda","year":"2019","journal-title":"J. CO2 Util."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"012023","DOI":"10.1088\/1755-1315\/290\/1\/012023","article-title":"Environmental impact of concrete and concrete-based construction waste leachates","volume":"290","author":"Sackey","year":"2019","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_7","unstructured":"APA. (Portuguese Environment Agency) (2015, April 02). (In Portuguese)."},{"key":"ref_8","unstructured":"Santos, A. (2014). Occupational Toxicology\u2014Toxicity of Organic Solvents, Portuguese Chemical Society. (In Portuguese)."},{"key":"ref_9","unstructured":"Nassis, C. (2015, April 02). (In Portuguese)."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1002\/tox.20187","article-title":"Application of leaching tests for toxicity evaluation of coal fly ash","volume":"21","author":"Tsiridis","year":"2006","journal-title":"Environ. Toxicol."},{"key":"ref_11","unstructured":"(2002). Characterisation of Waste. Leaching. Compliance Test for Leaching of Granular Waste Materials and Sludges. Part 2: One Stage Batch Test at a Liquid to Solid Ratio of 10 L\/kg for Materials with Particle Size below 4 mm (without or with Size Reduction), Comit\u00e9 Europ\u00e9en de Normalisation. EN 12457-2:2002."},{"key":"ref_12","unstructured":"(1992). Toxicity Characteristic Leaching Procedure, TCLP 1311:1992."},{"key":"ref_13","first-page":"41","article-title":"Evaluation of daphnia magna for the ecotoxicity assessment of alkali leachate from concrete","volume":"26","author":"Choi","year":"2013","journal-title":"Int. J. Indust. Entomol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.scitotenv.2014.09.094","article-title":"Suitability of a Saccharomyces cerevisiae-based assay to assess the toxicity of pyrimethanil sprayed soils via surface runoff: Comparison with standard aquatic and soil toxicity assays","volume":"505","author":"Gil","year":"2015","journal-title":"Sci. Total Environ."},{"key":"ref_15","unstructured":"Kanare, H.M., and West, B.W. (1993). Leachability of selected chemical elements from concrete. Emerging Technologies Symposium on Cement and Concrete in the Global Environment, Portland Cement Association."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0166-1116(08)70411-4","article-title":"Leaching tests for concrete containing fly ash-Evaluation and mechanism","volume":"48","author":"Rankers","year":"1991","journal-title":"Stud. Environ. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0008-8846(98)00200-2","article-title":"Long-term leaching of toxic trace metals from Portland cement concrete","volume":"29","author":"Hillier","year":"1991","journal-title":"Cement Concrete Res."},{"key":"ref_18","unstructured":"(1980). Council Directive 80\/778\/EEC of 15 July 1980 Relating to the Quality of Water Intended for Human Consumption\u2014Repealed by Council Directive 98\/83\/EC on the Quality of Water Intended for Human Consumption."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.wasman.2015.12.029","article-title":"Evaluation of heavy metal leaching from coal ash-versus conventional concrete monoliths and debris","volume":"49","author":"Gwenzi","year":"2006","journal-title":"J. Waste Manag."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00611","DOI":"10.1016\/j.heliyon.2018.e00611","article-title":"Toxicity and environmental and economic performance of fly ash and recycled concrete aggregates use in concrete: A review","volume":"4","author":"Kurda","year":"2018","journal-title":"Heliyon"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Rodrigues, P., Silvestre, J.D., Flores-Colen, I., Viegas, C.A., de Brito, J., Kurad, R., and Demertzi, M. (2017). Methodology for the assessment of the ecotoxicological potential of construction materials. Materials, 10.","DOI":"10.3390\/ma10060649"},{"key":"ref_22","unstructured":"(2008). Directive 2008\/98\/EC of the European Parliament and of the Council of 19 November 2008 on Waste and Repealing Certain Directives (Text with EEA Relevance), European Parliament and Council of the European Union."},{"key":"ref_23","unstructured":"(1999). Directive 1999\/31\/EC of 26 April 1999 on the landfill of waste. Off. J. Eur. Communities, L182, 1\u201319."},{"key":"ref_24","unstructured":"(2003). Council decision 2003\/33\/EC of the European Community of 19 December 2002 Establishing Criteria and Procedures for the Acceptance of Waste and Landfills Pursuant to Article 16 of and Annex II to Directive 1999\/31\/EC. Off. J. Eur. Communities, L11, 27\u201349."},{"key":"ref_25","unstructured":"(1998). Criteria on the Evaluation Methods of Waste Ecotoxicity: Proposal\u2014CEMWE, ADEME. ADEME 1998."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jhazmat.2006.12.064","article-title":"Chemical and ecotoxicological characterization of ashes obtained from sewage sludge combustion in a fluidised-bed reactor","volume":"147","author":"Lapa","year":"2007","journal-title":"J. Hazard Mater."},{"key":"ref_27","unstructured":"(2013). Tests of the Mechanical and Physical Properties of the Aggregates. Part 6: Determination of Density and Water Absorption, Comit\u00e9 Europ\u00e9en de Normalisation. EN 1097-6:2013."},{"key":"ref_28","unstructured":"(1998). Tests for mechanical and physical properties of aggregates. Methods for the Determination of Resistance to Fragmentation, Comit\u00e9 Europ\u00e9en de Normalisation. EN 1097-2:1998."},{"key":"ref_29","unstructured":"(2002). Tests to Determine the Geometric Characteristics of the Aggregates. Part 4: Determination of the Shape of the Particles, Form index; Comit\u00e9 Europ\u00e9en de Normalisation. EN 933-4:2002."},{"key":"ref_30","unstructured":"(2002). Aggregates for Concrete, Instituto Portugu\u00eas da Qualidade. EN 12620:2002+A1:2010."},{"key":"ref_31","unstructured":"(2002). Characterization of Waste. Leaching\u2014Compliance Test for Leaching of Granular Waste Materials and Sludges. Part 4: One Stage Batch Test at a Liquid to Solid Ratio of 10 L\/kg for Materials with Particle Size below 10 mm (without or with Size Reduction), Comit\u00e9 Europ\u00e9en de Normalisation. EN 12457-4:2002."},{"key":"ref_32","unstructured":"(2007). Water Quality\u2014Determination of Selected Elements by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), International Organization for Standardization. ISO 11885:2007."},{"key":"ref_33","unstructured":"(2017). Determination of Anions by Ion Chromatograph. Standard Methods for the Examination of Water and Wastewater, American Public Health Association, American Water Works Association and Water Environment Federation. SMEWW 4110:2017."},{"key":"ref_34","unstructured":"(2017). Solids. Standard Methods for the Examination of Water and Wastewater, American Public Health Association, American Water Works Association and Water Environment Federation. SMEWW 2540:2017."},{"key":"ref_35","unstructured":"(2017). Total Organic Carbon (TOC). Standard Methods for the Examination of Water and Wastewater, American Public Health Association, American Water Works Association and Water Environment Federation. SMEWW 5310:2017."},{"key":"ref_36","unstructured":"(2017). H+ pH value. Standard Methods for the Examination of Water and Wastewater, American Public Health Association, American Water Works Association and Water Environment Federation. SMEWW 4500:2017."},{"key":"ref_37","unstructured":"(2017). Boron. Standard Methods for the Examination of Water and Wastewater, American Public Health Association, American Water Works Association and Water Environment Federation. SMEWW 4500:2017."},{"key":"ref_38","unstructured":"(1996). Determination of Conductivity\u2014Condutimetry, Instituto Portugu\u00eas da Qualidade. (In Portuguese)."},{"key":"ref_39","unstructured":"(2007). Water Quality\u2014Determination of the Inhibitory Effect of Water Samples on the Light Emission of Vibrio Fischeri (Luminescent Bacteria Test). Part 3: Method Using Freeze Dried Bacteria, International Organization for Standardization. ISO 11348-3:2007."},{"key":"ref_40","unstructured":"(2012). Determination of the Inhibition of the Mobility of Daphnia Magna Straus (Cladocera, Crustacea)\u2014Acute Toxicity Test, International Organization for Standardization. ISO 6341:2012."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/tox.10141","article-title":"A practical and user-friendly toxicity classification system with microbiotests for natural waters and wastewaters","volume":"18","author":"Persoone","year":"2003","journal-title":"Environ. Toxicol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/j.wasman.2012.02.005","article-title":"Correlation analysis between sulphate content and leaching of sulphates in recycled aggregates from construction and demolition wastes","volume":"32","author":"Barbudo","year":"2012","journal-title":"J. Waste Manag."},{"key":"ref_43","unstructured":"(2006). Regulation (EC) 1907\/2006 of 18 December 2006, of the European Parliament concerning the Registration, evaluation, authorization and restriction of chemicals (REACH). Off. J. Eur. Union, L396, 1\u2013849."},{"key":"ref_44","unstructured":"(2008). Regulation (EC) 1272\/2008 of the European Parliament and of the Council of 16 December 2008 on Classification, Labelling and Packaging of Substances and Mixtures, Europa EU."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.fuel.2004.06.038","article-title":"Physico-chemical characteristics of European pulverized coal combustion fly ashes","volume":"84","author":"Moreno","year":"2005","journal-title":"Fuel"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"974","DOI":"10.2136\/sssaj1974.03615995003800060038x","article-title":"Boron availability as influenced by application of fly ash to soil","volume":"38","author":"Plank","year":"1974","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_47","first-page":"83","article-title":"Physical and chemical properties of fly ash from coal-fired power plants with special reference to environmental impacts","volume":"71","author":"Page","year":"1979","journal-title":"Residue Rev."},{"key":"ref_48","unstructured":"(2008). Directive 2008\/105\/EC of the European Parliament and of the Council of 16 December 2008 on Environmental Quality Standards in the Field of Water Policy, Europa EU."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.ecoenv.2012.07.011","article-title":"Environmental hazard assessment of coal fly ashes using leaching and ecotoxicity tests","volume":"84","author":"Tsiridis","year":"2012","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_50","unstructured":"(2008). Directive 2000\/60\/EC of the European Parliament and of the Council Establishing a Framework for the Community Action in the Field of Water Policy, Europa EU."}],"container-title":["Applied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3417\/10\/1\/351\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:18:46Z","timestamp":1760361526000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3417\/10\/1\/351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,3]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["app10010351"],"URL":"https:\/\/doi.org\/10.3390\/app10010351","relation":{},"ISSN":["2076-3417"],"issn-type":[{"value":"2076-3417","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,3]]}}}