{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T08:29:03Z","timestamp":1768292943189,"version":"3.49.0"},"reference-count":55,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,4,27]],"date-time":"2020-04-27T00:00:00Z","timestamp":1587945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portugal 2020","award":["023310 \u2013 ALGACO2"],"award-info":[{"award-number":["023310 \u2013 ALGACO2"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}]},{"name":"CRESC Algarve 2020 and COMPETE 2020","award":["EMBRC.PT ALG-01-0145-FEDER-022121"],"award-info":[{"award-number":["EMBRC.PT ALG-01-0145-FEDER-022121"]}]},{"name":"CRESC Algarve 2020 and COMPETE 2020","award":["BIODATA.PT ALG-01-0145-FEDER-022231"],"award-info":[{"award-number":["BIODATA.PT ALG-01-0145-FEDER-022231"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g\u00b7m\u22122\u00b7day\u22121) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g\u00b7m\u22122\u00b7day\u22121). Chlorococcum amblystomatis displayed high protein content (48%\u201356% dry weight (DW)) and moderate levels of total lipids (18%\u201331% DW), carbohydrates (6%\u201318% DW) and ashes (9%\u201316% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg\u00b7g\u22121 DW, followed by lutein with 5.37 mg\u00b7g\u22121 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids.<\/jats:p>","DOI":"10.3390\/app10093040","type":"journal-article","created":{"date-parts":[[2020,4,28]],"date-time":"2020-04-28T05:05:32Z","timestamp":1588050332000},"page":"3040","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5411-8686","authenticated-orcid":false,"given":"N\u00e1dia","family":"Correia","sequence":"first","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department\u2014Rua 25 de Abril, n\u00ba 19, 2445-413 Pataias, Portugal"},{"name":"MARE\u2014Marine and Environmental Sciences Centre, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1369-2099","authenticated-orcid":false,"given":"Hugo","family":"Pereira","sequence":"additional","affiliation":[{"name":"CCMAR\u2014Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Joana T.","family":"Silva","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department\u2014Rua 25 de Abril, n\u00ba 19, 2445-413 Pataias, Portugal"}]},{"given":"Tam\u00e1ra","family":"Santos","sequence":"additional","affiliation":[{"name":"CCMAR\u2014Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Maria","family":"Soares","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department\u2014Rua 25 de Abril, n\u00ba 19, 2445-413 Pataias, Portugal"}]},{"given":"Carolina B.","family":"Sousa","sequence":"additional","affiliation":[{"name":"CCMAR\u2014Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, Portugal"},{"name":"Global Health and Tropical Medicine (GHMT), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, 1349-008 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0961-5397","authenticated-orcid":false,"given":"Lisa M.","family":"Sch\u00fcler","sequence":"additional","affiliation":[{"name":"CCMAR\u2014Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9147-8621","authenticated-orcid":false,"given":"Margarida","family":"Costa","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department\u2014Rua 25 de Abril, n\u00ba 19, 2445-413 Pataias, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3101-693X","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Varela","sequence":"additional","affiliation":[{"name":"CCMAR\u2014Centre of Marine Sciences, University of Algarve, Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6819-0619","authenticated-orcid":false,"given":"Leonel","family":"Pereira","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal"}]},{"given":"Joana","family":"Silva","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department\u2014Rua 25 de Abril, n\u00ba 19, 2445-413 Pataias, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.plipres.2006.01.001","article-title":"Lipids and lipid metabolism in eukaryotic algae","volume":"45","author":"Guschina","year":"2006","journal-title":"Prog. 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