{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:45:45Z","timestamp":1777632345488,"version":"3.51.4"},"reference-count":29,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T00:00:00Z","timestamp":1590710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["xx"],"award-info":[{"award-number":["xx"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>The effect of different acid sulfite pretreatment conditions on released components in the hydrolysates and the pretreated solid residues\u2019 response to enzymatic hydrolysis for Eucalyptus globulus chips was investigated. Sodium bisulfite (0\u201315%), and sulfuric acid (0\u20135%) were used to pretreat chips at 170 \u00b0C and 190 \u00b0C, for as long as 30 min. The hydrolysates were analyzed through high-performance liquid chromatography (HPLC) and spectrophotometry. Overall porosity and pores larger than 2.65 nm (size of a typical cellulase) on the solid residues were estimated using glucose and two dextrans with different hydrodynamic radii as probes. The external specific surface area was analyzed by dynamic light scattering. The solid residues underwent enzymatic hydrolysis with an enzymatic cocktail. Very high (84\u201395%) carbohydrate conversion was achieved for either an extensively delignified biomass or a biomass with very high content of sulfonated residual lignin (23.4%), since internal porosity enables enzymes accessibility. At least 5% sodium bisulfite and 1% sulfuric acid was required to attain a carbohydrate release above 90% in the enzymatic hydrolysis. Results suggest that the presence of sulfonated lignin does not impair the enzymatic hydrolysis rate and extent. The increase of pretreatment temperature had a positive effect mainly on the initial rate of carbohydrates release in the enzymatic hydrolysis. The increase of the wood material dimensions from pins to conventional chips significantly decreased the hemicellulose removal in acid sulfite pretreatment but had a small effect on the enzymatic yield.<\/jats:p>","DOI":"10.3390\/app10113764","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T11:49:21Z","timestamp":1591012161000},"page":"3764","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Comprehensive Approach of Eucalyptus globulus Acid Sulfite Pretreatment for Enzymatic Hydrolysis"],"prefix":"10.3390","volume":"10","author":[{"given":"Vera","family":"D. Costa","sequence":"first","affiliation":[{"name":"Fiber Materials and Environmental Technologies unit (FibEnTech-UBI), Universidade da Beira Interior, 6201-001 Covilh\u00e3, Portugal"}]},{"given":"Ana","family":"Costa","sequence":"additional","affiliation":[{"name":"Fiber Materials and Environmental Technologies unit (FibEnTech-UBI), Universidade da Beira Interior, 6201-001 Covilh\u00e3, Portugal"}]},{"given":"Maria","family":"Amaral","sequence":"additional","affiliation":[{"name":"Fiber Materials and Environmental Technologies unit (FibEnTech-UBI), Universidade da Beira Interior, 6201-001 Covilh\u00e3, Portugal"}]},{"given":"Rog\u00e9rio","family":"S. Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Fiber Materials and Environmental Technologies unit (FibEnTech-UBI), Universidade da Beira Interior, 6201-001 Covilh\u00e3, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1111\/j.1467-7652.2009.00482.x","article-title":"Evaluation of plant biomass resources available for replacement of fossil oil","volume":"8","author":"Henry","year":"2010","journal-title":"Plant Biotechnol. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.biortech.2014.01.127","article-title":"Integration of the first and second generation bioethanol processes and the importance of by-products","volume":"165","author":"Lennartsson","year":"2014","journal-title":"Bioresour. 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