{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T14:06:01Z","timestamp":1776521161081,"version":"3.51.2"},"reference-count":33,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T00:00:00Z","timestamp":1615334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Sergipe (FAPITEC), Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq, #443238\/2014-6, #470388\/2014-5) and Banco do Nordeste (FUNDECI\/2017.0014), the Portuguese Science and Technology Foundation","award":["UIDB\/04469\/2020 (strategic fund)"],"award-info":[{"award-number":["UIDB\/04469\/2020 (strategic fund)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>Entomopathogenic fungi are microbial agents of insect control in nature. They have been used as biologic strategies to manage insect invasion; however, the challenge is to maintain their shelf life and viability when exposed to high temperatures, ultraviolet radiation, and humidity. Synthesized silver nanoparticles (AgNPs) from fungal extracellular enzymes are an alternative using these microorganisms to obtain nanoparticles with insecticidal action. The present study evaluates the biomass production and the potential to synthesize silver nanoparticles using entomopathogenic fungi isolates. Sixteen isolates of entomopathogenic fungi were used in this study. The fungi pathogenicity and virulence were evaluated using the insect model Tenebrio molitor, at a concentration of 5 \u00d7 106 conidia\/mL. The fungal biomass was produced in a liquid medium, dried, and weighed. The synthesis of silver nanoparticles was performed with aqueous extracts of the entomopathogenic fungi and silver nitrate solution (1 mM), following characterization by a UV\/vis spectrophotometer, mean size, and polydispersity index. The results showed a significant variation in pathogenicity, virulence, and biomass production among the evaluated fungi isolates; however, only one of the isolates did not have the potential to synthesize silver nanoparticles. Pearson\u2019s correlation showed significant correlation values only between virulence \u00d7 biosynthesis potential and biomass production \u00d7 biosynthesis potential, both with negative values, indicating an inverse correlation. Thus, AgNPs with entomopathogenic fungus extract can produce an innovative bioinsecticide product using a green production process.<\/jats:p>","DOI":"10.3390\/app11062465","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T13:27:20Z","timestamp":1615382840000},"page":"2465","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Entomopathogenic Fungi Biomass Production and Extracellular Biosynthesis of Silver Nanoparticles for Bioinsecticide Action"],"prefix":"10.3390","volume":"11","author":[{"given":"T\u00e1rcio S.","family":"Santos","sequence":"first","affiliation":[{"name":"University of Tiradentes (Unit) Av, Murilo Dantas, 300, 49010-390 Aracaju, Sergipe, Brazil"},{"name":"Institute of Technology and Research (ITP) Av, Murilo Dantas, 300, 49010-390 Aracaju, Sergipe, Brazil"}]},{"given":"Eliana M. dos","family":"Passos","sequence":"additional","affiliation":[{"name":"Sergipe Agricultural Development Company (Emdagro), Av. Carlos Rodrigues da Cruz s\/n, Aracaju, 49081-015 Sergipe, Brazil"}]},{"given":"Matheus G. de Jesus","family":"Seabra","sequence":"additional","affiliation":[{"name":"University of Tiradentes (Unit) Av, Murilo Dantas, 300, 49010-390 Aracaju, Sergipe, Brazil"}]},{"given":"Eliana B.","family":"Souto","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Coimbra, P\u00f3lo das Ci\u00eancias da Sa\u00fade, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal"},{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6527-6612","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Severino","sequence":"additional","affiliation":[{"name":"University of Tiradentes (Unit) Av, Murilo Dantas, 300, 49010-390 Aracaju, Sergipe, Brazil"},{"name":"Institute of Technology and Research (ITP) Av, Murilo Dantas, 300, 49010-390 Aracaju, Sergipe, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6003-4796","authenticated-orcid":false,"given":"Marcelo da Costa","family":"Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"University of Tiradentes (Unit) Av, Murilo Dantas, 300, 49010-390 Aracaju, Sergipe, Brazil"},{"name":"Institute of Technology and Research (ITP) Av, Murilo Dantas, 300, 49010-390 Aracaju, Sergipe, Brazil"},{"name":"Sergipe Agricultural Development Company (Emdagro), Av. Carlos Rodrigues da Cruz s\/n, Aracaju, 49081-015 Sergipe, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Khan, M.R., Fromm, K.M., Rizvi, T.F., Giese, B., Ahamad, F., Turner, R.J., F\u00fceg, M., and Marsili, E. (2020). Metal Nanoparticle\u2013Microbe Interactions: Synthesis and Antimicrobial Effects. Part. Part. Syst. Charact., 1900419.","DOI":"10.1002\/ppsc.201900419"},{"key":"ref_2","unstructured":"Moraga, E.Q. (2020). Entomopathogenic fungi as endophytes: Their broader contribution to IPM and crop production. Biocontrol Sci. Technol., 1\u201314."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Mora, M.A.E., Castilho, A.M.C., and Fraga, M.E. (2017). Classification and infection mechanism of entomopathogenic fungi. Arq. Inst. 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