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This work aims to characterize the value-added compounds extracted from Curcubita pepo seeds using green methodologies, namely microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE), employing water as an extracting solvent for two ratios (condition 1: 1 mg\/20 mL; condition 2: 2.5 mg\/20 mL). The extract with the best antioxidant\/antiradical activity in FRAP (71.09 \u03bcmol FSE\/g DW) and DPPH (5.08 mg TE\/g DW) assays was MAE condition 1, while MAE condition 2 exhibited the highest activity in the ABTS assay (13.29 mg AAE\/g DW) and TPC (16.89 mg GAE\/g DW). A remarkable scavenging capacity was observed, particularly for HOCl, with IC50 values ranging from 1.88\u201313.50 \u03bcg\/mL. A total of 21 phenolic compounds were identified, being catechin (4.567\u20137.354 mg\/g DW), caffeine (1.147\u20132.401 mg\/g DW) and gallic acid (0.945\u20131.337 mg\/g DW) predominant. No adverse effects were observed on Caco-2 viability after exposure to MAE extracts, while the other conditions led to a slight viability decrease in NSC-34. These results highlighted that the extract from MAE condition 2 is the most promising as a potential nutraceutical ingredient.<\/jats:p>","DOI":"10.3390\/app12031763","type":"journal-article","created":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T01:03:40Z","timestamp":1644368620000},"page":"1763","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Microwave- and Ultrasound-Assisted Extraction of Cucurbita pepo Seeds: A Comparison Study of Antioxidant Activity, Phenolic Profile, and In-Vitro Cells Effects"],"prefix":"10.3390","volume":"12","author":[{"given":"Catarina","family":"Macedo","sequence":"first","affiliation":[{"name":"REQUIMTE\/LAQV-Polytechnique of Porto-School of Engineering, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1823-9816","authenticated-orcid":false,"given":"Ana Margarida","family":"Silva","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV-Polytechnique of Porto-School of Engineering, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 431, 4249-015 Porto, Portugal"}]},{"given":"Ana Sofia","family":"Ferreira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV-Polytechnique of Porto-School of Engineering, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1500-9211","authenticated-orcid":false,"given":"Manuela M.","family":"Moreira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV-Polytechnique of Porto-School of Engineering, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3924-776X","authenticated-orcid":false,"given":"Cristina","family":"Delerue-Matos","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV-Polytechnique of Porto-School of Engineering, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8803-0041","authenticated-orcid":false,"given":"Francisca","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV-Polytechnique of Porto-School of Engineering, Rua Dr. Ant\u00f3nio Bernardino de Almeida, 431, 4249-015 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,8]]},"reference":[{"key":"ref_1","unstructured":"Boisrobert, C.E., Stjepanovic, A., Oh, S., and Lelieveld, H.L.M. 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