{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T07:06:07Z","timestamp":1776582367151,"version":"3.51.2"},"reference-count":98,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["PD \/ BD \/ 150567 \/ 2020"],"award-info":[{"award-number":["PD \/ BD \/ 150567 \/ 2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/50017\/2020+UIDB\/50017\/2020+ LA\/P\/0094\/2020"],"award-info":[{"award-number":["UIDP\/50017\/2020+UIDB\/50017\/2020+ LA\/P\/0094\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"\u201cAgencia Estatal de Investigaci\u00f3n\u201d of the Ministry of Science and Innovation of Spain and by the IDAEA-CSIC, the Centro de Your Excellency Severo Ochoa","award":["CEX2018-000794-S"],"award-info":[{"award-number":["CEX2018-000794-S"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>Fluoxetine is an antidepressant widely used to treat depressive and anxiety states. Due to its mode of action in the central nervous system (selective serotonin reuptake inhibitor (SSRI)), it becomes toxic to non-target organisms, leading to changes that are harmful to their survival. In this work, the effects of fluoxetine on juvenile zebrafish (Danio rerio) were evaluated, assessing biochemical (phase II biotransformation\u2014glutathione S-transferase (GST), neurotransmission\u2014acetylcholinesterase (ChE), energy metabolism\u2014lactate dehydrogenase (LDH), and oxidative stress\u2014glutathione peroxidase (GPx)) and behavior endpoints (swimming behavior, social behavior, and thigmotaxis) after 21 days exposure to 0 (control), 0.1, 1 and 10 \u00b5g\/L. Biochemically, although chronic exposure did not induce significant effects on neurotransmission and energy metabolism, GPx activity was decreased after exposure to 10 \u00b5g\/L of fluoxetine. At a behavioral level, exploratory and social behavior was not affected. However, changes in the swimming pattern of exposed fish were observed in light and dark periods (decreased locomotor activity). Overall, the data show that juvenile fish chronically exposed to fluoxetine may exhibit behavioral changes, affecting their ability to respond to environmental stressors and the interaction with other fish.<\/jats:p>","DOI":"10.3390\/app12042256","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:24:21Z","timestamp":1645475061000},"page":"2256","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Chronic Effects of Fluoxetine on Danio rerio: A Biochemical and Behavioral Perspective"],"prefix":"10.3390","volume":"12","author":[{"given":"Daniela","family":"Correia","sequence":"first","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Biology, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"given":"In\u00eas","family":"Domingues","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Biology, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]},{"given":"Melissa","family":"Faria","sequence":"additional","affiliation":[{"name":"Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Jordi Girona 18, 08034 Barcelona, Spain"}]},{"given":"Miguel","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Biology, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.jocn.2021.07.023","article-title":"Investigating the potential mechanisms of depression induced-by COVID-19 infection in patients","volume":"91","author":"Mohammadkhanizadeh","year":"2021","journal-title":"J. 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