{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T20:22:50Z","timestamp":1774556570829,"version":"3.50.1"},"reference-count":81,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T00:00:00Z","timestamp":1648857600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>This paper presents a potentiometric biosensor for the detection of amyloid \u03b2-42 (A\u03b2-42) in point-of-care analysis. This approach is based on the molecular imprint polymer (MIP) technique, which uses covalently immobilised A\u03b2-42 to create specific detection cavities on the surface of single-walled carbon nanotubes (SWCNTs). The biosensor was prepared by binding A\u03b2-42 to the SWCNT surface and then imprinting it by adding acrylamide (monomer), N,N\u2032-methylene-bis-acrylamide (crosslinker) and ammonium persulphate (initiator). The target peptide was removed from the polymer matrix by the proteolytic action of an enzyme (proteinase K). The presence of imprinting sites was confirmed by comparing a MIP-modified surface with a negative control (NIP) consisting of a similar material where the target molecule had been removed from the process. The ability of the sensing material to rebind A\u03b2-42 was demonstrated by incorporating the MIP material as an electroactive compound in a PVC\/plasticiser mixture applied to a solid conductive support of graphite. All steps of the synthesis of the imprinted materials were followed by Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR). The analytical performance was evaluated by potentiometric transduction, and the MIP material showed cationic slopes of 75 mV-decade\u22121 in buffer pH 8.0 and a detection limit of 0.72 \u03bcg\/mL. Overall, potentiometric transduction confirmed that the sensor can discriminate A\u03b2-42 in the presence of other biomolecules in the same solution.<\/jats:p>","DOI":"10.3390\/app12073625","type":"journal-article","created":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T02:59:52Z","timestamp":1648954792000},"page":"3625","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection"],"prefix":"10.3390","volume":"12","author":[{"given":"S\u00f3nia Cla\u00fadia","family":"Ribeiro","sequence":"first","affiliation":[{"name":"BioMark Sensor Research\u2014ISEP, School of Engineering, Polytechnic Institute of Porto, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8933-3984","authenticated-orcid":false,"given":"R\u00faben","family":"Fernandes","sequence":"additional","affiliation":[{"name":"LaBMI, Laboratory of Medical and Industrial Biotechnology, Polytechnic Institute of Porto, 4249-015 Porto, Portugal"},{"name":"HEFP, FCS-UFP\u2014Fernando Pessoa Hospital, Faculty of Health Sciences, Fernando Pessoa University, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4237-8952","authenticated-orcid":false,"given":"Felismina T. C.","family":"Moreira","sequence":"additional","affiliation":[{"name":"BioMark Sensor Research\u2014ISEP, School of Engineering, Polytechnic Institute of Porto, 4249-015 Porto, Portugal"},{"name":"CEB, Centre of Biological Engineering, University of Minho, 4715-000 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9936-7336","authenticated-orcid":false,"given":"Maria Goreti Ferreira","family":"Sales","sequence":"additional","affiliation":[{"name":"BioMark Sensor Research\u2014ISEP, School of Engineering, Polytechnic Institute of Porto, 4249-015 Porto, Portugal"},{"name":"CEB, Centre of Biological Engineering, University of Minho, 4715-000 Braga, Portugal"},{"name":"BioMark, Sensor Research@UC, Department of Chemical Engineering, Faculty of Science and Technology, Coimbra University, 3030-790 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1111\/joim.12816","article-title":"Biomarkers for Alzheimer\u2019s disease: Current status and prospects for the future","volume":"284","author":"Blennow","year":"2018","journal-title":"J. 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