{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T11:58:53Z","timestamp":1765886333571,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T00:00:00Z","timestamp":1648944000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04046\/2020","PTDC\/Bia-BQM\/28355\/2017","SFRH\/BD\/135692\/2018","UID\/AMB\/00681\/2020"],"award-info":[{"award-number":["UIDB\/04046\/2020","PTDC\/Bia-BQM\/28355\/2017","SFRH\/BD\/135692\/2018","UID\/AMB\/00681\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>Cynara cardunculus L. inflorescence infusion has been used for several centuries as curd in traditional cheese making, such as some highly prized Portuguese cheeses. To promote the sustainable use of all C. cardunculus plants, C. cardunculus extract leaves decoction (CL), inflorescence decoction (CI), chlorogenic acid (CA) (a compound in the plant leaves), and rosmarinic acid (RA) (a similar phenolic compound) solutions were tested for antimicrobial activity against bacteria that may appear on the cheese rind. The antimicrobial activity was evaluated by 15 bacterial strains using two different methodologies: solid and liquid. The influence of these extracts and the phenolic compounds on melanin bioproduction by Pseudomonas putida ESACB 191 was also studied. CA and RA (1 mg\/mL) showed antimicrobial activity. CL and CA reduced P. putida ESACB 191 growth in the liquid assay and melanin bioproduction by 6.20 Log CFU\/mL and 50%, respectively. Cynarin, CA, and its derivates were identified as the main phenolic compounds (52%) of CL, which may justify its inhibitory action on bacterial growth and melanin bioproduction. Thus, future perspectives include the application of CL extracts with antimicrobial activity in edible films and\/or coatings to applied in cheese rind to increase the shelf time.<\/jats:p>","DOI":"10.3390\/app12073629","type":"journal-article","created":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T06:04:01Z","timestamp":1648965841000},"page":"3629","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Influence of Cynara cardunculus L. Phenolic Compounds on Pseudomonas putida Isolated from the Dairy Industry: Growth and Melanin Bioproduction"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8356-907X","authenticated-orcid":false,"given":"Ana Rita","family":"Ferraz","sequence":"first","affiliation":[{"name":"Instituto de Biossistemas e Ci\u00eancias Integrativas (BioISI), Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"},{"name":"Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5433-4258","authenticated-orcid":false,"given":"Cristina M. B. S.","family":"Pintado","sequence":"additional","affiliation":[{"name":"Escola Superior Agr\u00e1ria, Instituto Polit\u00e9cnico de Castelo Branco, Quinta da Sra. de M\u00e9rcoles, 6001-909 Castelo Branco, Portugal"},{"name":"CERNAS\/IPCB, Centro de Estudos de Recursos Naturais, Ambiente e Sociedade\/Instituto Polit\u00e9cnico de Castelo Branco, 6001-909 Castelo Branco, Portugal"},{"name":"QRural\u2014Unidade de Investiga\u00e7\u00e3o Qualidade de Vida no Mundo Rural, Instituto Polit\u00e9cnico de Castelo Branco, Av. Pedro \u00c1lvares Cabral, n\u00ba 12, 6000-084 Castelo Branco, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7541-9613","authenticated-orcid":false,"given":"Maria Lu\u00edsa","family":"Serralheiro","sequence":"additional","affiliation":[{"name":"Instituto de Biossistemas e Ci\u00eancias Integrativas (BioISI), Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"},{"name":"Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103937","DOI":"10.1016\/j.jff.2020.103937","article-title":"Cynara cardunculus L.: Outgoing and potential trends of phytochemical, industrial, nutritive and medicinal merits","volume":"69","author":"Zayed","year":"2020","journal-title":"J. Funct. 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