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Several species are industrially produced and commercialized for the feed or cosmetic industries, however, other applications in the functional food and pharmaceutical markets can be foreseen. In this study, nine industrial\/commercial species were evaluated for in vitro antioxidant, calcium-chelating, anti-tumoral, and anti-inflammatory activities. The most promising extracts were fractionated yielding several promising fractions namely, of Tetraselmis striata CTP4 with anti-inflammatory activity (99.0 \u00b1 0.8% reduction in TNF-\u03b1 production in LPS stimulated human macrophages at 50 \u00b5g\/mL), of Phaeodactylum Tricornutum with cytotoxicity towards cancerous cell lines (IC50 = 22.3 \u00b1 1.8 \u03bcg\/mL and 27.5 \u00b1 1.6 \u03bcg\/mL for THP-1 and HepG2, respectively) and of Porphyridium sp. and Skeletonema sp. with good chelating activity for iron, copper and calcium (IC50 = 0.047, 0.272, 0.0663 mg\/mL and IC50 = 0.055, 0.240, 0.0850 mg\/mL, respectively). These fractions were chemically characterized by GC\u2013MS after derivatization and in all, fatty acids at various degrees of unsaturation were the most abundant compounds. Some of the species under study proved to be potentially valuable sources of antioxidant, metal chelators, anti-tumoral and anti-inflammatory compounds with possible application in the functional food and pharmaceutical industries.<\/jats:p>","DOI":"10.3390\/app12125877","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T10:49:14Z","timestamp":1654771754000},"page":"5877","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Microalgae as Potential Sources of Bioactive Compounds for Functional Foods and Pharmaceuticals"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5368-5475","authenticated-orcid":false,"given":"M\u00e9lanie","family":"Silva","sequence":"first","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Farah","family":"Kamberovic","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Sisay Tesema","family":"Uota","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Ismael-Mohammed","family":"Kovan","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Carla S. B.","family":"Viegas","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"GenoGla Diagnostics, Centre of Marine Sciences (CCMAR), Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5145-4753","authenticated-orcid":false,"given":"Dina C.","family":"Simes","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"GenoGla Diagnostics, Centre of Marine Sciences (CCMAR), Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"given":"Katkam N.","family":"Gangadhar","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3101-693X","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Varela","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"Green Colab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4077-855X","authenticated-orcid":false,"given":"Lu\u00edsa","family":"Barreira","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"Green Colab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"574111","DOI":"10.3389\/fpubh.2020.574111","article-title":"Management and prevention strategies for non-communicable diseases (NCDs) and their risk factors","volume":"8","author":"Budreviciute","year":"2020","journal-title":"Front. 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Impact of volatile anesthetics on oxidative stress and inflammation. BioMed Res. Int., 2015.","DOI":"10.1155\/2015\/242709"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Dales, J.-P., and Desplat-J\u00e9go, S. (2020). Metal imbalance in neurodegenerative diseases with a specific concern to the brain of multiple sclerosis patients. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21239105"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3109\/00207454.2016.1174118","article-title":"Brain biometals and Alzheimer\u2019s disease\u2014Boon or bane?","volume":"127","author":"Prakash","year":"2017","journal-title":"Int. J. Neurosci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Sheikh, S., Haque, E., and Mir, S.S. (2013). Neurodegenerative diseases: Multifactorial conformational diseases and their therapeutic interventions. J. Neurodegener. Dis., 2013.","DOI":"10.1155\/2013\/563481"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"593116","DOI":"10.3389\/fphar.2021.593116","article-title":"Medicinal prospects of antioxidants from algal sources in cancer therapy","volume":"12","author":"Ferdous","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.3390\/md9101860","article-title":"Cnidarians as a Source of new marine bioactive compounds\u2014An overview of the last decade and future steps for bioprospecting","volume":"9","author":"Rocha","year":"2011","journal-title":"Mar. Drugs"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Wahab, M., Zahoor, M., Muhammad Salman, S., Kamran, A.W., Naz, S., Burlakovs, J., Kallistova, A., Pimenov, N., and Zekker, I. (2021). Adsorption-membrane hybrid approach for the removal of azithromycin from water: An attempt to minimize drug resistance problem. Water, 13.","DOI":"10.3390\/w13141969"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Morais, E.G., Cristofoli, N.L., Maia, I.B., Magina, T., Cerqueira, P.R., Teixeira, M.R., Varela, J., Barreira, L., and Gouveia, L. (2021). Microalgal systems for wastewater treatment: Technological trends and challenges towards waste recovery. Energies, 14.","DOI":"10.3390\/en14238112"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Montero-Lobato, Z., V\u00e1zquez, M., Navarro, F., Fuentes, J.L., Bermejo, E., Garbayo, I., V\u00edlchez, C., and Cuaresma, M. (2018). Chemically-Induced production of anti-inflammatory molecules in microalgae. Mar. Drugs, 16.","DOI":"10.3390\/md16120478"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"58","DOI":"10.3389\/fnut.2018.00058","article-title":"Trends in microalgae incorporation into innovative food products with potential health benefits","volume":"5","author":"Caporgno","year":"2018","journal-title":"Front. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"278","DOI":"10.3389\/fmars.2020.00278","article-title":"A new network for the advancement of marine biotechnology in Europe and beyond","volume":"7","author":"Rotter","year":"2020","journal-title":"Front. Mar. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"628597","DOI":"10.3389\/fbioe.2021.628597","article-title":"Advantages of heterotrophic microalgae as a host for phytochemicals production","volume":"9","author":"Jareonsin","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"914","DOI":"10.3389\/fbioe.2020.00914","article-title":"Bioengineering of microalgae: Recent advances, perspectives, and regulatory challenges for industrial application","volume":"8","author":"Kumar","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.biortech.2016.03.060","article-title":"Atmospheric CO2 capture by algae: Negative carbon dioxide emission path","volume":"215","author":"Moreira","year":"2016","journal-title":"Bioresour. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"516","DOI":"10.3389\/fmicb.2016.00516","article-title":"Discovery of bioactive metabolites in biofuel microalgae that offer protection against predatory bacteria","volume":"7","author":"Bagwell","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3670","DOI":"10.1016\/j.bbagen.2013.02.008","article-title":"Natural products: A continuing source of novel drug leads","volume":"1830","author":"Cragg","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/s2175-97902018000117280","article-title":"Antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis\u2014An in vitro assay","volume":"54","author":"Ebrahimzadeh","year":"2018","journal-title":"Braz. J. Pharm. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Conde, T.A., Neves, B.F., Couto, D., Melo, T., Neves, B., Costa, M., Silva, J., Domingues, P., and Domingues, M.R. (2021). Microalgae as sustainable bio-factories of healthy lipids: Evaluating fatty acid content and antioxidant activity. Mar. Drugs, 19.","DOI":"10.3390\/md19070357"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1515\/chem-2016-0029","article-title":"Microalgae-based unsaponifiable matter as source of natural antioxidants and metal chelators to enhance the value of wet Tetraselmis chuii biomass","volume":"14","author":"Gangadhar","year":"2016","journal-title":"Open Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4845","DOI":"10.1007\/s12649-021-01349-6","article-title":"Anti-cholesterol and antioxidant activities of independent and combined microalgae aqueous extracts in vitro","volume":"12","author":"Ibrahim","year":"2021","journal-title":"Waste Biomass Valorization"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Casas-Arrojo, V., Decara, J., de Los \u00c1ngeles Arrojo-Agudo, M., P\u00e9rez-Manr\u00edquez, C., and Abdala-D\u00edaz, R.T. (2021). Immunomodulatory, antioxidant activity and cytotoxic effect of sulfated polysaccharides from Porphyridium cruentum. (S.F.Gray) N\u00e4geli. Biomolecules, 11.","DOI":"10.3390\/biom11040488"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1007\/s40200-020-00681-3","article-title":"Evaluation of the anti-inflammatory and antioxidant effects of the microalgae Nannochloropsis gaditana in streptozotocin-induced diabetic rats","volume":"19","author":"Nacer","year":"2020","journal-title":"J. Diabetes Metab. Disord."},{"key":"ref_27","first-page":"365","article-title":"Evaluation of antioxidant properties, total phenolic and carotenoid content of Chaetoceros calcitrans, Chlorella salina and Isochrysis galbana","volume":"3","author":"Saranya","year":"2014","journal-title":"Int. J. Curr. Microbiol. App.Sci."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Neumann, U., Derwenskus, F., Flaiz Flister, V., Schmid-Staiger, U., Hirth, T., and Bischoff, S.C. (2019). Fucoxanthin, A carotenoid derived from Phaeodactylum tricornutum exerts antiproliferative and antioxidant activities in vitro. Antioxidants, 8.","DOI":"10.3390\/antiox8060183"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.foodchem.2011.08.047","article-title":"Microalgae of different phyla display antioxidant, metal chelating and acetylcholinesterase inhibitory activities","volume":"131","author":"Justo","year":"2012","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"43","DOI":"10.22159\/ajpcr.2016.v9i6.14507","article-title":"Pharmaceutically valuable bioactive compounds of algae","volume":"9","author":"Bhattacharjee","year":"2016","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s10811-017-1156-9","article-title":"Effects of salinity on the CO2 permeation across lipid bilayer for microalgae biofixation: A molecular dynamics study","volume":"30","author":"Manrique","year":"2018","journal-title":"J. Appl. Phycol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"71628","DOI":"10.1039\/C6RA15051A","article-title":"Exploring saccharinate-tetrazoles as selective Cu(II) ligands: Structure, magnetic properties and cytotoxicity of copper(II) complexes based on 5-(3-aminosaccharyl)-tetrazoles","volume":"6","author":"Ismael","year":"2016","journal-title":"RSC Adv."},{"key":"ref_33","first-page":"S23","article-title":"Nanoencapsulation as a novel delivery approach for therapeutic applications of gla-rich protein (GRP)","volume":"53","author":"Viegas","year":"2021","journal-title":"Ann. Med."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Ara\u00fajo, N., Viegas, C.S.B., Zub\u00eda, E., Magalh\u00e3es, J., Ramos, A., Carvalho, M.M., Cruz, H., Sousa, J.P., Blanco, F.J., and Vermeer, C. (2020). Amentadione from the alga Cystoseira usneoides as a novel osteoarthritis protective agent in an ex vivo co-culture OA model. Mar. Drugs, 18.","DOI":"10.3390\/md18120624"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.indcrop.2017.10.038","article-title":"Solvent selection for efficient extraction of bioactive compounds from grape pomace","volume":"111","author":"Beara","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.jfda.2013.11.001","article-title":"Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica","volume":"22","author":"Do","year":"2014","journal-title":"J. Food Drug Anal."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.foodchem.2004.09.037","article-title":"Optimization of accelerated solvent extraction of antioxidants from Spirulina platensis microalga","volume":"93","author":"Herrero","year":"2005","journal-title":"Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Aminina, N.M., Karaulova, E.P., Vishnevskaya, T.I., Yakush, E.V., Kim, Y.-K., Nam, K.-H., and Son, K.-T. (2020). Characteristics of polyphenolic content in brown algae of the Pacific coast of Russia. Mol. Basel Switz., 25.","DOI":"10.3390\/molecules25173909"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jbiotec.2016.11.026","article-title":"Antioxidant capacities of fucoxanthin-producing algae as influenced by their carotenoid and phenolic contents","volume":"241","author":"Foo","year":"2017","journal-title":"J. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"103","DOI":"10.4028\/www.scientific.net\/MSF.964.103","article-title":"Lipids extraction from wet and unbroken microalgae Chlorella vulgaris using subcritical water","volume":"964","author":"Zullaikah","year":"2019","journal-title":"Mater. Sci. Forum"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Ardiles, P., Cerezal-Mezquita, P., Salinas-Fuentes, F., \u00d3rdenes, D., Renato, G., and Ruiz-Dom\u00ednguez, M.C. (2020). Biochemical composition and phycoerythrin extraction from red microalgae: A comparative study using green extraction technologies. Processes, 8.","DOI":"10.3390\/pr8121628"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.jbiotec.2014.10.016","article-title":"Light modulation of biomass and macromolecular composition of the diatom Skeletonema marinoi","volume":"192","author":"Chandrasekaran","year":"2014","journal-title":"J. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.carbpol.2018.07.037","article-title":"Advances in exopolysaccharides based bioremediation of heavy metals in soil and water: A critical review","volume":"199","author":"Kranthi","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"012006","DOI":"10.1088\/1755-1315\/160\/1\/012006","article-title":"Phycobiliproteins production and heavy metals reduction ability of Porphyridium sp.","volume":"160","author":"Priatni","year":"2018","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"43","DOI":"10.3233\/AJW200034","article-title":"Microalgae Skeletonema costatum for Cd and Cu remediation","volume":"17","author":"Pratiwi","year":"2020","journal-title":"Asian J. Water Environ. Pollut."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.foodchem.2015.02.147","article-title":"Determination of reducing power and metal chelating ability of antioxidant peptides: Revisited methods","volume":"183","author":"Kessler","year":"2015","journal-title":"Food Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.2174\/1385272819666150709165210","article-title":"Several classes of natural products with metal ion chelating ability","volume":"19","author":"Shi","year":"2015","journal-title":"Curr. Org. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Gulcin, \u0130., and Alwasel, S.H. (2022). Metal ions, metal chelators and metal chelating assay as antioxidant method. Processes, 10.","DOI":"10.3390\/pr10010132"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"4659","DOI":"10.1038\/s41598-022-08380-0","article-title":"Discovery of calcium-binding peptides derived from defatted lemon basil seeds with enhanced calcium uptake in human intestinal epithelial cells, caco-2","volume":"12","author":"Kheeree","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"487","DOI":"10.2147\/NDT.S350939","article-title":"Role of calcium homeostasis in Alzheimer\u2019s Disease","volume":"18","author":"Ge","year":"2022","journal-title":"Neuropsychiatr. Dis. Treat."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.ceca.2017.06.008","article-title":"Calcium signaling and molecular mechanisms underlying neurodegenerative diseases","volume":"70","author":"Pchitskaya","year":"2018","journal-title":"Cell Calcium"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e1704","DOI":"10.7717\/peerj.1704","article-title":"Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds","volume":"4","author":"Acosta","year":"2016","journal-title":"PeerJ"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1517\/17425255.2.2.183","article-title":"Hepatocyte cell lines: Their use, scope and limitations in drug metabolism studies","volume":"2","author":"Castell","year":"2006","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Saide, A., Damiano, S., Ciarcia, R., and Lauritano, C. (2021). Promising Activities of marine natural products against hematopoietic malignancies. Biomedicines, 9.","DOI":"10.3390\/biomedicines9060645"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.steroids.2017.03.006","article-title":"Synthesis and anti-cancer activity of chiral tetrahydropyrazolo[1,5-a]pyridine-fused steroids","volume":"122","author":"Lopes","year":"2017","journal-title":"Steroids"},{"key":"ref_56","first-page":"1066","article-title":"Constituents from roots of Maytenus distichophylla, antimicrobial activity and toxicity for cells and Caenorhabditis elegans","volume":"43","author":"Morales","year":"2020","journal-title":"Qu\u00edm. Nova"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.2174\/0929867322666150209150639","article-title":"A simple and reliable approach for assessing anticancer activity in vitro","volume":"22","year":"2015","journal-title":"Curr. Med. Chem."},{"key":"ref_58","first-page":"1","article-title":"Small molecules in targeted cancer therapy: Advances, challenges, and future perspectives","volume":"6","author":"Zhong","year":"2021","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1007\/s00449-019-02273-9","article-title":"Improvement of carotenoid extraction from a recently isolated, robust microalga, Tetraselmis sp. CTP4 (Chlorophyta)","volume":"43","author":"Gangadhar","year":"2020","journal-title":"Bioprocess Biosyst. Eng."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"469","DOI":"10.3389\/fbioe.2020.00469","article-title":"Isolation and characterization of novel Chlorella vulgaris mutants with low chlorophyll and improved protein contents for food applications","volume":"8","author":"Machado","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"125385","DOI":"10.1016\/j.biortech.2021.125385","article-title":"Carotenoid biosynthetic gene expression, pigment and n-3 fatty acid contents in carotenoid-rich Tetraselmis striata CTP4 strains under heat stress combined with high light","volume":"337","author":"Bombo","year":"2021","journal-title":"Bioresour. Technol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Almutairi, A.W. (2020). Improvement of chemical composition of Tisochrysis lutea grown mixotrophically under nitrogen depletion towards biodiesel production. Molecules, 25.","DOI":"10.3390\/molecules25204609"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1021\/np800124k","article-title":"Apoptosis-inducing galactolipids from a cultured marine diatom, Phaeodactylum tricornutum","volume":"71","author":"Andrianasolo","year":"2008","journal-title":"J. Nat. Prod."},{"key":"ref_64","first-page":"4500","article-title":"Omega-3 polyunsaturated fatty acid inhibits the malignant progression of hepatocarcinoma by inhibiting the wnt\/\u03b2-catenin pathway","volume":"22","author":"Chang","year":"2018","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Hussein, H.A., and Abdullah, M.A. (2020). Anticancer compounds derived from marine diatoms. Mar. Drugs, 18.","DOI":"10.3390\/md18070356"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Gangadhar, K.N., Rodrigues, M.J., Pereira, H., Gaspar, H., Malcata, F.X., Barreira, L., and Varela, J. (2020). Anti-hepatocellular carcinoma (HepG2) activities of monoterpene hydroxy lactones isolated from the marine microalga Tisochrysis lutea. Mar. Drugs, 18.","DOI":"10.3390\/md18110567"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Le Goff, M., Delbrut, A., Quinton, M., Pradelles, R., Bescher, M., Burel, A., Schoefs, B., Sergent, O., Lagadic-Gossmann, D., and Le Ferrec, E. (2020). Protective action of Ostreococcus tauri and Phaeodactylum tricornutum extracts towards benzo[a]pyrene-induced cytotoxicity in endothelial cells. Mar. Drugs, 18.","DOI":"10.3390\/md18010003"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.chroma.2016.06.015","article-title":"Substitution of carcinogenic solvent dichloromethane for the extraction of volatile compounds in a fat-free model food system","volume":"1456","author":"Cayot","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1475-2859-13-14","article-title":"A Comparative study: The impact of different lipid extraction methods on current microalgal lipid research","volume":"13","author":"Li","year":"2014","journal-title":"Microb. Cell Factories"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Xia, D., Qiu, W., Wang, X., and Liu, J. (2021). Recent advancements and future perspectives of microalgae-derived pharmaceuticals. Mar. Drugs, 19.","DOI":"10.3390\/md19120703"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Ulusoy Erol, H.B., Menegazzo, M.L., Sandefur, H., Gottberg, E., Vaden, J., Asgharpour, M., Hestekin, C.N., and Hestekin, J.A. (2020). Porphyridium cruentum grown in ultra-filtered swine wastewater and its effects on microalgae growth productivity and fatty acid composition. Energies, 13.","DOI":"10.3390\/en13123194"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Sharmin, T., Monirul Hasan, C.M., Aftabuddin, S., Rahman, M.A., and Khan, M. (2016). Growth, fatty acid, and lipid composition of marine microalgae Skeletonema costatum available in bangladesh coast: Consideration as biodiesel feedstock. J. Mar. Biol., 2016.","DOI":"10.1155\/2016\/6832847"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.egypro.2017.10.251","article-title":"Analyzing Phaeodactylum tricornutum lipid profile for biodiesel production","volume":"136","author":"Martin","year":"2017","journal-title":"Energy Procedia"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1007\/s10499-019-00489-w","article-title":"Lipid composition and some bioactivities of 3 newly isolated microalgae (Tetraselmis sp. IMP3, Tetraselmis sp. CTP4, and Skeletonema sp.)","volume":"28","author":"Cardoso","year":"2020","journal-title":"Aquac. Int."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Calviello, G., Su, H.-M., Weylandt, K.H., Fasano, E., Serini, S., and Cittadini, A. (2013). Experimental evidence of -3 polyunsaturated fatty acid modulation of inflammatory cytokines and bioactive lipid mediators: Their potential role in inflammatory, neurodegenerative, and neoplastic diseases. BioMed Res. Int., 2013.","DOI":"10.1155\/2013\/743171"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1080\/1028415X.2017.1290928","article-title":"Neuroprotective effect of omega-3 polyunsaturated fatty acids in the 6-OHDA model of Parkinson\u2019s disease is mediated by a reduction of inducible nitric oxide synthase","volume":"21","author":"Mori","year":"2018","journal-title":"Nutr. Neurosci."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"El-Fayoumy, E.A., Shanab, S.M.M., Gaballa, H.S., Tantawy, M.A., and Shalaby, E.A. (2021). Evaluation of antioxidant and anticancer activity of crude extract and different fractions of Chlorella vulgaris axenic culture grown under various concentrations of copper ions. BMC Complement. Med. Ther., 21.","DOI":"10.1186\/s12906-020-03194-x"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1007\/s10295-016-1814-y","article-title":"Biobased production of alkanes and alkenes through metabolic engineering of microorganisms","volume":"44","author":"Kang","year":"2017","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_79","first-page":"1865","article-title":"Phytochemical and antioxidant studies of leaf of Tetrapleura tetraptera (schum and thon) taubert (mimosaceae)","volume":"4","author":"Enema","year":"2019","journal-title":"Brifish J. Pharm. Med. Res."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"313","DOI":"10.22159\/ajpcr.2018.v11i10.25577","article-title":"Identification of bioactive components in Enhalus acoroides seagrass extract by gas chromatography\u2013mass spectrometry","volume":"11","author":"Parthasarathy","year":"2018","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/3-540-48255-5_1","article-title":"Cyclopropane derivatives and their diverse biological activities","volume":"Volume 207","year":"2000","journal-title":"Small Ring Compounds in Organic Synthesis VI"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"7645","DOI":"10.3390\/molecules17077645","article-title":"Butylated hydroxytoluene analogs: Synthesis and evaluation of their multipotent antioxidant activities","volume":"17","author":"Yehye","year":"2012","journal-title":"Molecules"},{"key":"ref_83","first-page":"1229","article-title":"Synthesis and antimicrobial evaluation of 4H-pyrans and schiff bases fused 4H-pyran derivatives as inhibitors of Mycobacterium bovis (BCG)","volume":"17","author":"Mahdavi","year":"2018","journal-title":"Iran. J. Pharm. Res. IJPR"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"4231","DOI":"10.3390\/md13074231","article-title":"Advances in microalgae-derived phytosterols for functional food and pharmaceutical applications","volume":"13","author":"Luo","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_85","first-page":"775","article-title":"Anti-inflammation effects of 8-Oxo-9-octadecenoic acid isolated from Undaria peterseniana in lipopolysaccharide-stimulated macrophage cells","volume":"17","author":"Kang","year":"2018","journal-title":"EXCLI J."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Bigagli, E., D\u2019Ambrosio, M., Cinci, L., Niccolai, A., Biondi, N., Rodolfi, L., Dos Santos Nascimiento, L.B., Tredici, M.R., and Luceri, C. (2021). A Comparative in vitro evaluation of the anti-inflammatory effects of a Tisochrysis lutea extract and fucoxanthin. Mar. 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