{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T20:55:38Z","timestamp":1769720138984,"version":"3.49.0"},"reference-count":56,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T00:00:00Z","timestamp":1656979200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fungi4Health project","award":["NORTE-01-0247-FEDER-070171"],"award-info":[{"award-number":["NORTE-01-0247-FEDER-070171"]}]},{"name":"Fungi4Health project","award":["NORTE 2020 (North Regional Operational Program 2014\/2020)"],"award-info":[{"award-number":["NORTE 2020 (North Regional Operational Program 2014\/2020)"]}]},{"name":"European Regional Development Fund (ERDF)","award":["NORTE-01-0247-FEDER-070171"],"award-info":[{"award-number":["NORTE-01-0247-FEDER-070171"]}]},{"name":"European Regional Development Fund (ERDF)","award":["NORTE 2020 (North Regional Operational Program 2014\/2020)"],"award-info":[{"award-number":["NORTE 2020 (North Regional Operational Program 2014\/2020)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>This work aimed to evaluate the potential of three fungi strains, Lentinula edodes, Pleurotus eryngii, and Pleurotus citrinopileatus, to degrade lignin and enhance the nutritive value of grape stalks (GS). The GS was inoculated with the fungi and incubated under solid-state fermentation at 28 \u00b0C and 85% relative humidity for 7, 14, 21, 28, 35, and 42 days, in an incubation chamber. The influence of the treatments was evaluated by analyzing the potential modifications in the chemical composition, in vitro organic matter digestibility (IVOMD) and enzymatic kinetics. An increase (p &lt; 0.001) in the crude protein content was observed in the GS treated with L. edodes and P. citrinopileatus at 42 days of incubation (50 and 75%, respectively). The treatment performed with L. edodes decreased (p &lt; 0.001) lignin content by 52%, and led to higher (p &lt; 0.001) IVOMD values at 42 days of incubation. By contrast, P. eryngii did not affect lignin content and IVOMD. A higher activity of all enzymes was also detected for the treatment with L. edodes. Results indicated that L. edodes has a great potential to enhance the nutritive value of GS as an animal feed, due to its lignin degradation selectivity.<\/jats:p>","DOI":"10.3390\/app12136800","type":"journal-article","created":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T10:21:33Z","timestamp":1657016493000},"page":"6800","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Biovalorization of Grape Stalks as Animal Feed by Solid State Fermentation Using White-Rot Fungi"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8852-5113","authenticated-orcid":false,"given":"Val\u00e9ria","family":"Costa-Silva","sequence":"first","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), Department of Animal Science, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Mariana","family":"Anuncia\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), Department of Animal Science, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Ederson","family":"Andrade","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Federal University of Bahia, Salvador 40170110, Brazil"}]},{"given":"Lisete","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Chemistry Department (ECVA), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Aida","family":"Costa","sequence":"additional","affiliation":[{"name":"Floresta Viva, Golas Street, 466-748 Amarante, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5481-8770","authenticated-orcid":false,"given":"Irene","family":"Fraga","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5834-6141","authenticated-orcid":false,"given":"Ana","family":"Barros","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0963-5785","authenticated-orcid":false,"given":"Guilhermina","family":"Marques","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3341-861X","authenticated-orcid":false,"given":"Lu\u00eds","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0049-6424","authenticated-orcid":false,"given":"Miguel","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1016\/j.wasman.2011.06.001","article-title":"Valorization of winery waste vs. the costs of not recycling","volume":"31","author":"Vecino","year":"2011","journal-title":"Waste Manag."},{"key":"ref_2","first-page":"1","article-title":"Exploring the potential of wine industry by-products as source of additives to improve the quality of aquafeed","volume":"155","author":"Silva","year":"2020","journal-title":"Microchem. 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