{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T22:51:59Z","timestamp":1771455119838,"version":"3.50.1"},"reference-count":23,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T00:00:00Z","timestamp":1660262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EnergyPulse Systems"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>Essential oils, EOs, are concentrated liquids with complex mixtures of volatile organic compounds that can be extracted from different plant materials. EOs have been used by humans throughout history due to their natural properties: from pleasant fragrances, to anti-bacterial\/fungal activities. This work presents the impact of pulsed electric fields, PEF, application as a pre-treatment for the extraction of EOs from eucalyptus, rosemary, and thyme leaves. The initial PEF pre-treatment was first applied to eucalyptus and rosemary leaves two weeks after harvesting, with a 2 kV\/cm electric field and a specific energy of approximately 10 kJ\/kg, followed by EO extraction by hydrodistillation, HD, with distillation times of 30 and 60 min. The best results were obtained for PEF pre-treated samples and 30 min HD, exhibiting an increasing trend in the average extraction yield of approximately 17% and 11% for eucalyptus and rosemary, respectively, in comparison with no PEF applied. The composition of the EOs extracted from eucalyptus was analyzed for their total phenolic content, TPC, where PEF pre-treated samples showed a higher polyphenol extraction, reaching 30% for 30 min HD. Finally, the optimization of the PEF pre-treatment was also studied, for maximizing the quantity of EO extracted from dry thyme leaves, while aiming for a minimization of energy consumption, for different distillation times. For this study it was observed that, for this plant material, an electric field of 1 kV\/cm with 0.4 kJ\/kg and an HD time of 30 min, after PEF application, was able to achieve an extraction yield up to 40% higher than the conventional method.<\/jats:p>","DOI":"10.3390\/app12168107","type":"journal-article","created":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T21:09:06Z","timestamp":1660511346000},"page":"8107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Extraction of Essential Oils from Plants by Hydrodistillation with Pulsed Electric Fields (PEF) Pre-Treatment"],"prefix":"10.3390","volume":"12","author":[{"given":"Maria","family":"Barros","sequence":"first","affiliation":[{"name":"IST-UL, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2381-4627","authenticated-orcid":false,"given":"Lu\u00eds","family":"Redondo","sequence":"additional","affiliation":[{"name":"GIAAPP\/ISEL, Pulsed Power Advanced Applications Group, Lisbon Engineering Superior Institute, R. Conselheiro Em\u00eddio Navarro 1, 1959-007 Lisbon, Portugal"}]},{"given":"Duarte","family":"Rego","sequence":"additional","affiliation":[{"name":"EPS, EnergyPulse Systems, Lispolis, 1600-546 Lisbon, Portugal"}]},{"given":"Cesleste","family":"Serra","sequence":"additional","affiliation":[{"name":"Lisbon Engineering Superior Institute, R. Conselheiro Em\u00eddio Navarro 1, 1959-007 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6888-383X","authenticated-orcid":false,"given":"Kadour","family":"Miloudi","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Systems and Geomatics Research, Mascara University, Mascara 29000, Algeria"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.fct.2007.09.106","article-title":"Biological effects of essential oils\u2014A review","volume":"46","author":"Bakkali","year":"2008","journal-title":"Food Chem. Toxicol."},{"key":"ref_2","first-page":"205","article-title":"Chemical composition and antimicrobial activity of essential oil of thyme (Thymus vulgaris) from eastern Morocco","volume":"11","author":"Imelouane","year":"2009","journal-title":"Int. J. Agric. Biol."},{"key":"ref_3","first-page":"9","article-title":"Comparison extraction methods of essential oils of Rosmarinus officinalis L. In Iran by microwave assisted water distillation; water distillation and steam distillation","volume":"7","author":"Jaimand","year":"2018","journal-title":"J. Med. Plants By-Prod."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Tisserand, R. (2014). Essential Oil Safety, Elsevier. [2nd ed.].","DOI":"10.1016\/B978-0-443-06241-4.00013-8"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"R1231","DOI":"10.1111\/1750-3841.12492","article-title":"Essential Oils: Extraction, Bioactivities, and Their Uses for Food Preservation","volume":"79","author":"Tongnuanchan","year":"2014","journal-title":"J. Food Sci."},{"key":"ref_6","unstructured":"Badal, S., and Delgoda, R. (2017). Pharmacognosy: Fundamentals, Applications and Strategies. Pharmacognosy, Elsevier."},{"key":"ref_7","first-page":"127","article-title":"Effect of pulsed electric fields (PEF) on oil yield and quality during distillation of white oil-bearing rose (Rosa alba L.)","volume":"15","author":"Dobreva","year":"2010","journal-title":"Z. Fur Arznei Gewurzpflanzen"},{"key":"ref_8","first-page":"117","article-title":"Techniques for Extraction of Essential Oils from Plants: A Review","volume":"10","author":"Rassem","year":"2016","journal-title":"Aust. J. Basic Appl. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1109\/MEI.2012.6268438","article-title":"Cell membrane electroporation\u2014Part 1: The phenomenon","volume":"28","author":"Kotnik","year":"2012","journal-title":"IEEE Electr. Insul. Mag."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s13197-020-04512-4","article-title":"The application of PEF technology in food processing and human nutrition","volume":"58","author":"Nowosad","year":"2020","journal-title":"J. Food Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Akiyama, H., and Heller, R. (2017). Environmental Applications, Food and Biomass Processing by Pulsed Electric Fields. Bioelectrics, Springer.","DOI":"10.1007\/978-4-431-56095-1"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1007\/s41614-021-00059-9","article-title":"Pulsed power applications for agriculture and food processing","volume":"5","author":"Takaki","year":"2021","journal-title":"Rev. Mod. Plasma Phys."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.3389\/fbioe.2019.00265","article-title":"Perspective on Pulsed Electric Field Treatment in the Bio-based Industry","volume":"7","author":"Buchmann","year":"2019","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"639150","DOI":"10.3389\/fbioe.2021.639150","article-title":"Editorial: Pulsed Electric Fields in Biotechnology","volume":"9","author":"Vorobiev","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.biortech.2018.01.068","article-title":"Electric field-based technologies for valorization of bioresources","volume":"254","author":"Rocha","year":"2018","journal-title":"Bioresour. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/tee.23282","article-title":"Pulsed Discharge Plasmas in Contact with Water and their Applications","volume":"16","author":"Akiyama","year":"2021","journal-title":"IEEJ Trans. Electr. Electron. Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1109\/MEI.2013.6410537","article-title":"Cell Membrane Electroporation\u2014Part 2: The Applications","volume":"29","author":"Haberl","year":"2013","journal-title":"IEEE Electr. Insul. Mag."},{"key":"ref_18","first-page":"1255","article-title":"Effect of pulsed electric fields on distillation of essential oil crops. Comptes rendus l\u2019Acad\u00e9mie Bulg","volume":"66","author":"Dobreva","year":"2013","journal-title":"CR Acad. Bulg. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1080\/0972060X.2018.1484820","article-title":"Intensification of Essential Oil Extraction of the Marrubium vulgare Using Pulsed Electric Field","volume":"21","author":"Miloudi","year":"2018","journal-title":"J. Essent. Oil Bear. Plants"},{"key":"ref_20","first-page":"295","article-title":"Effects of high intensity pulsed electric fields on yield and chemical composition of rose essential oil","volume":"10","author":"Zhou","year":"2017","journal-title":"Int. J. Agric. Biol. Eng."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Miloudi, K., Tilmatine, A., Benmimoun, Y., Hamimed, A., Taibi, A., and Bellebna, Y. (2018, January 28\u201331). Intensification of Essential Oil Extraction of Artemisia herba alba Using Pulsed Electric Field. Proceedings of the 2018 International Conference on Electrical Sciences and Technologies in Maghreb (CISTEM), Algiers, Algeria.","DOI":"10.1109\/CISTEM.2018.8613580"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1109\/TPS.2009.2023221","article-title":"Repetitive High-Voltage Solid-State Marx Modulator Design for Various Load Conditions","volume":"37","author":"Redondo","year":"2009","journal-title":"IEEE Trans. Plasma Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."}],"container-title":["Applied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3417\/12\/16\/8107\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:08:09Z","timestamp":1760141289000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3417\/12\/16\/8107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,12]]},"references-count":23,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["app12168107"],"URL":"https:\/\/doi.org\/10.3390\/app12168107","relation":{},"ISSN":["2076-3417"],"issn-type":[{"value":"2076-3417","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,12]]}}}