{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:45:50Z","timestamp":1761396350142,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T00:00:00Z","timestamp":1661558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordena\u00e7\u00e3o Aperfei\u00e7oamento de Pessoal de Nivel Superior (CAPES)","award":["88887.159533\/2017-00","301964\/2019-0","06\/2019","n\u00ba 01\/2019"],"award-info":[{"award-number":["88887.159533\/2017-00","301964\/2019-0","06\/2019","n\u00ba 01\/2019"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Sergipe (FAPITEC)","award":["88887.159533\/2017-00","301964\/2019-0","06\/2019","n\u00ba 01\/2019"],"award-info":[{"award-number":["88887.159533\/2017-00","301964\/2019-0","06\/2019","n\u00ba 01\/2019"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["88887.159533\/2017-00","301964\/2019-0","06\/2019","n\u00ba 01\/2019"],"award-info":[{"award-number":["88887.159533\/2017-00","301964\/2019-0","06\/2019","n\u00ba 01\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>Formononetin suppresses catabolic effects in primary rat chondrocytes induced by IL-1\u03b2, which makes it a promising candidate for in vivo studies on the treatment and prevention of osteoarthritis (OA). The goal of this study is to investigate the effects of the oral administration of formononetin in a rodent model of OA. OA was induced by medial meniscectomy in the right knee joint of rats. The animals were assigned into four groups (n = 6): Vehicle (treated with saline), FNT10 (formononetin, 10 mg\/kg), Ibuprofen (10 mg\/kg), and Sham (simulated surgery, treated with saline). The treatment of the animals was performed daily by the oral route. After six weeks, the knee joints were removed and histologically processed. Histological sections stained in Safranin-O were used to assess the histological grading of the articular cartilage damage. An analysis of the immunohistochemical expression of type II collagen and IL-1\u03b2 was also performed. The oral administration of formononetin significantly reduced cartilage-matrix-loss width (p &lt; 0.01), degeneration scores (p &lt; 0.05), and the total articular cartilage-wear depth (p &lt; 0.01) in comparison with Group Vehicle. Type II collagen immunoexpression was intense and homogeneous in FNT10, comparable to that of Sham, scarce and irregularly distributed in Vehicle, and homogeneous but less intense in Ibuprofen. Furthermore, formononetin significantly reduced the immunohistochemical expression of IL-1\u03b2 in joint chondrocytes (p &lt; 0.01), but ibuprofen did not (p &gt; 0.05). From this study, the oral administration of formononetin was found to attenuate OA-associated pathological damage in rodents, likely because of IL-1\u03b2 expression downregulation in chondrocytes. These findings suggest that formononetin is a potential therapeutic for treatment.<\/jats:p>","DOI":"10.3390\/app12178591","type":"journal-article","created":{"date-parts":[[2022,8,28]],"date-time":"2022-08-28T21:22:56Z","timestamp":1661721776000},"page":"8591","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Application of Formononetin for the Treatment of Knee Osteoarthritis Induced by Medial Meniscectomy in a Rodent Model"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8500-1904","authenticated-orcid":false,"given":"Ronald B.","family":"Barreto","sequence":"first","affiliation":[{"name":"Department of Medicine, Tiradentes University, Aracaju 49032-490, SE, Brazil"}]},{"given":"Bruna H. de","family":"Santana","sequence":"additional","affiliation":[{"name":"Department of Medicine, Tiradentes University, Aracaju 49032-490, SE, Brazil"}]},{"given":"Beatriz M.","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Medicine, Tiradentes University, Aracaju 49032-490, SE, Brazil"}]},{"given":"Erick S.","family":"Porto","sequence":"additional","affiliation":[{"name":"Department of Medicine, Tiradentes University, Aracaju 49032-490, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6527-6612","authenticated-orcid":false,"given":"Patricia","family":"Severino","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research (I.T.P.), Tiradentes University, Aracaju 49032-490, SE, Brazil"},{"name":"Biotechnological Postgraduate Program, Tiradentes University, Aracaju 49032-490, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0357-4144","authenticated-orcid":false,"given":"Juliana C.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research (I.T.P.), Tiradentes University, Aracaju 49032-490, SE, Brazil"},{"name":"Health and Environment Postgraduate Program, Tiradentes University, Aracaju 49032-490, SE, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9737-6017","authenticated-orcid":false,"given":"Eliana B.","family":"Souto","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, de Jorge Viterbo Ferreira n\u00ba. 228, 4050-313 Porto, Portugal"},{"name":"REQUIMTE\/UCIBIO, Faculty of Pharmacy, University of Porto, de Jorge Viterbo Ferreira n\u00ba. 228, 4050-313 Porto, Portugal"}]},{"given":"Ricardo L. C. de","family":"Albuquerque-J\u00fanior","sequence":"additional","affiliation":[{"name":"Institute of Technology and Research (I.T.P.), Tiradentes University, Aracaju 49032-490, SE, Brazil"},{"name":"Health and Environment Postgraduate Program, Tiradentes University, Aracaju 49032-490, SE, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1016\/j.foodchem.2008.08.004","article-title":"Variability of phytoestrogen content in foods from different sources","volume":"113","author":"Kuhnle","year":"2009","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1080\/00218839.2020.1714192","article-title":"Variation of the phytoestrogen composition of red propolis throughout the year","volume":"59","author":"Bataglion","year":"2020","journal-title":"J. Apic. 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