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Therefore, it is extremely difficult to control and boost the bioremediation of these systems after an oil spill. A mathematical model was developed to assist in the prediction and decision-making regarding the in situ bioremediation of hydrocarbon-contaminated soils. The model considered the most relevant processes involved in the mass transfer and biodegradation of alkanes over time and along the depth of a flooded soil column. Aliphatic hydrocarbons were chosen since they are less water soluble than aromatics and account for 50\u201390% of the hydrocarbon fraction in several petroleum products. The effect of adding oxygen, nitrate, iron (III) or sulfate as electron acceptors was then simulated (bioremediation scenarios). Additionally, and to feed the model, batch assays were performed to obtain experimental data on hydrocarbon adsorption to soil particles (more than 60% of hydrocarbons tends to be adsorbed to soil particles), as well as hydrocarbon biodegradation rates in the presence of nitrate (0.114 d\u22121) and oxygen (0.587 d\u22121). The model indicates that saturated hydrocarbon removal occurs mainly with adsorption\/desorption and transport processes in the upper layers of soil due to methanogenic biodegradation in deeper layers, since the other microbial processes are soon limited by the lack of electron acceptors. Simulation results show that higher initial electron acceptor concentrations led to higher hydrocarbon removal, confirming that the model is performing in accordance with the expected. Close to the surface (at 0.1 m depth), all scenarios predicted more than 83% hydrocarbon removal after two years of simulation. Soil re-aeration results in faster hydrocarbon removal (more than 20% after one year) and surfactants addition (around 15% after one year) may also accelerate soil bioremediation. With this model, the simultaneous contributions of the various physicochemical and biological processes are integrated, facilitating the simulation and comparison of different bioremediation scenarios. Therefore, it represents a useful support tool for the management of contaminated sites.<\/jats:p>","DOI":"10.3390\/app122111069","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T06:49:02Z","timestamp":1667371742000},"page":"11069","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A Mathematical Model for Bioremediation of Hydrocarbon-Contaminated Soils"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7187-0538","authenticated-orcid":false,"given":"Gilberto","family":"Martins","sequence":"first","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Sara","family":"Campos","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Ana","family":"Ferreira","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4229-8947","authenticated-orcid":false,"given":"Rita","family":"Castro","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4645-908X","authenticated-orcid":false,"given":"Maria Salom\u00e9","family":"Duarte","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7999-4620","authenticated-orcid":false,"given":"Ana J.","family":"Cavaleiro","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, Campus de Gualtar, 4710-057 Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,1]]},"reference":[{"key":"ref_1","unstructured":"P\u00e9rez, A.P., and Rodr\u00edguez Eugenio, N. (2018). Status of Local Soil Contamination in Europe\u2014Revision of the Indicator \u201cProgress in the Management Contaminated Sites in Europe\u201d, Publications Office of the European Union. EUR 29124 EN."},{"key":"ref_2","unstructured":"Van Liedekerke, M., Prokop, G., Rabl-Berger, S., Kibblewhite, M., and Louwagie, G. (2022, September 15). Progress in Management of Contaminated Sites, Available online: https:\/\/www.sepa.gov.rs\/download\/lbna26376enn.pdf."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3934\/microbiol.2017.1.25","article-title":"Soil bioremediation approaches for petroleum hydrocarbon polluted environments","volume":"3","author":"Koshlaf","year":"2017","journal-title":"AIMS Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1007\/s10661-012-2766-y","article-title":"Toxicity assessment for petroleum-contaminated soil using terrestrial invertebrates and plant bioassays","volume":"185","author":"Hentati","year":"2013","journal-title":"Environ. Monit. Assess."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ali, N., Bilal, M., Khan, A., Ali, F., and Iqbal, H.M.N. (2020). Effective exploitation of anionic, nonionic, and nanoparticle-stabilized surfactant foams for petroleum hydrocarbon contaminated soil remediation. Sci. Total Environ., 704.","DOI":"10.1016\/j.scitotenv.2019.135391"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.biortech.2016.10.037","article-title":"Microbial degradation of petroleum hydrocarbons","volume":"223","author":"Varjani","year":"2017","journal-title":"Bioresour. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/S1093-0191(02)00029-1","article-title":"A case study of bioremediation of petroleum-hydrocarbon contaminated soil at a crude oil spill site","volume":"7","author":"Gogoi","year":"2003","journal-title":"Adv. Environ. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.jenvman.2018.04.115","article-title":"Remediation of saline soils contaminated with crude oil using the halophyte Salicornia persica in conjunction with hydrocarbon-degrading bacteria","volume":"219","author":"Ebadi","year":"2018","journal-title":"J. Environ. Manag."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"35304","DOI":"10.1039\/C9RA06726D","article-title":"Bioremediation of petroleum hydrocarbon-contaminated soil by petroleum-degrading bacteria immobilized on biochar","volume":"9","author":"Zhang","year":"2019","journal-title":"RSC Adv."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/S0045-6535(99)00218-0","article-title":"Monitoring of bioremediation by soil biological activities","volume":"40","author":"Margesin","year":"2000","journal-title":"Chemosphere"},{"key":"ref_11","first-page":"1491","article-title":"Comparison of ex situ and in situ bioremediation of unsaturated soils contaminated by petroleum","volume":"36","author":"Carberry","year":"2001","journal-title":"J. Environ. Sci. Health-Part A Toxic\/Hazard. Subst. Environ. Eng."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ojewumi, M.E., Emetere, M.E., Babatunde, D.E., and Okeniyi, J.O. (2017). In Situ Bioremediation of Crude Petroleum Oil Polluted Soil Using Mathematical Experimentation. Int. J. Chem. Eng., 2017.","DOI":"10.1155\/2017\/5184760"},{"key":"ref_13","first-page":"135","article-title":"Review of Mathematical Modelling Techniques with Applications in Biosciences","volume":"3","author":"Alridha","year":"2022","journal-title":"Iraqi J. Comput. Sci. Math."},{"key":"ref_14","first-page":"276","article-title":"Kinetic modelling of a diesel-polluted clayey soil bioremediation process","volume":"557\u2013558","author":"Merlo","year":"2016","journal-title":"Sci. Total Environ."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.1029\/94WR01879","article-title":"Modeling biodegradation of residual petroleum in a saturated porous column","volume":"30","author":"Nicol","year":"1994","journal-title":"Water Resour. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.marpolbul.2014.04.007","article-title":"BIOB: A mathematical model for the biodegradation of low solubility hydrocarbons","volume":"83","author":"Geng","year":"2014","journal-title":"Mar. Pollut. Bull."},{"key":"ref_17","unstructured":"Weisman, W. (1998). Analysis of Petroleum Hydrocarbons in Environmental Media, Amherst Scientific Publishers."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0303-2647(00)00147-7","article-title":"Modelling of hexadecane degradation in continuous-flow cultures","volume":"59","year":"2001","journal-title":"BioSystems"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1134\/S1064229310060128","article-title":"Simulation of tridecane degradation under different soil water contents","volume":"43","author":"Kosterin","year":"2010","journal-title":"Eurasian Soil Sci."},{"key":"ref_20","first-page":"65","article-title":"Comparison of the Biodegradation of n-alkanes and Readily Biodegradable Substrates Using Open Mixed Culture under Aerobic, Anoxic and Anaerobic Conditions","volume":"5","author":"Uzukwu","year":"2017","journal-title":"Int. J. Environ. Bioremediat. Biodegrad."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1021\/es0019326","article-title":"Biodegradation of non-desorbable naphthalene in soils","volume":"35","author":"Park","year":"2001","journal-title":"Environ. Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1002\/1097-0290(20010405)73:1<12::AID-BIT1032>3.0.CO;2-W","article-title":"Evaluation of the interaction between biodegradation and sorption of phenanthrene in soil-slurry systems","volume":"73","author":"Woo","year":"2001","journal-title":"Biotechnol. Bioeng."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1061\/(ASCE)0733-9372(2001)127:8(748)","article-title":"Biodegradation of Naphthalene-Contaminated Soils in Slurry Bioreactors","volume":"127","author":"Cumaraswamy","year":"2001","journal-title":"J. Environ. Eng."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"21","DOI":"10.2166\/wst.1994.0025","article-title":"AQUASIM\u2014A tool for simulation and data analysis of aquatic systems","volume":"30","author":"Reichert","year":"1994","journal-title":"Water Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.1016\/j.apgeochem.2008.03.001","article-title":"Prospective scenarios for water quality and ecological status in Lake Sete Cidades (Portugal): The integration of mathematical modelling in decision processes","volume":"23","author":"Martins","year":"2008","journal-title":"Appl. Geochem."},{"key":"ref_26","unstructured":"Vera, L., Martel, G., Gutierrez, J., M\u00e1rquez, M., Abreu Acosta, N., Salas, J.J., Sard\u00f3n, N., Herrera Meli\u00e1n, J.A., Aguilar Bujalance, M.E., and Rexachs, J.A. (2006). Sustainable Management of Wastewater in Rural Zones: DEPURANAT Project (Gesti\u00f3n Sostenible del Agua Residual en Entornes Rurales: Proyecto DEPURANAT), Institute of the Canaries (Instituto Tecnol\u00f3gico de Can\u00e1rias)."},{"key":"ref_27","unstructured":"Reichert, P. (1998). Aquasim 2.0-User Manual, Computer Program for the Identification and Simulation of Aquatic Systems, EAWAG."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.copbio.2013.09.002","article-title":"Syntrophic biodegradation of hydrocarbon contaminants","volume":"27","author":"Gieg","year":"2014","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1159\/000441679","article-title":"Methanogenic Hydrocarbon Degradation: Evidence from Field and Laboratory Studies","volume":"26","author":"Richnow","year":"2016","journal-title":"Microb. Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4044","DOI":"10.1016\/j.biortech.2007.01.057","article-title":"Inhibition of anaerobic digestion process: A review","volume":"99","author":"Chen","year":"2008","journal-title":"Bioresour. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1021\/es00058a023","article-title":"Speciation of Fe(II) and Fe(III) in Contaminated Aquifer Sediments Using Chemical Extraction Techniques","volume":"28","author":"Heron","year":"1994","journal-title":"Environ. Sci. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s00254-007-0988-z","article-title":"Estimation of kinetic Monod parameters for anaerobic degradation of benzene in groundwater","volume":"55","author":"Vogt","year":"2008","journal-title":"Environ. Geol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.proenv.2011.09.006","article-title":"The Enhancement of Naphthalene Degradation in Soil by Hydroxypropyl-\u03b2-Cyclodextrin","volume":"10","author":"Dou","year":"2011","journal-title":"Procedia Environ. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Mielki, G.F., Novais, R.F., Ker, J.C.J.C., Verg\u00fctz, L., de Castro, G.F., Verg\u00fctz, L., and de Castro, G.F. (2016). Iron Availability in Tropical Soils and Iron Uptake by Plants. Rev. Bras. Ci\u00eanc. Solo, 40.","DOI":"10.1590\/18069657rbcs20150174"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1111\/j.1365-2389.1993.tb02328.x","article-title":"Simple determination of nitrate in soils by second-derivative spectroscopy","volume":"44","author":"Sempere","year":"1993","journal-title":"J. Soil Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/nrmicro1348","article-title":"Marine microorganisms make a meal of oil","volume":"4","author":"Head","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.apgeochem.2006.09.010","article-title":"Sorption isotherms: A review on physical bases, modeling and measurement","volume":"22","author":"Limousin","year":"2007","journal-title":"Appl. Geochem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1128\/jb.47.3.239-251.1944","article-title":"Studies of the Effect of Sodium Azide on Microbic Growth and Respiration: III. The Effect of Sodium Azide on the Gas Metabolism of B. subtilis and P. aeruginosa and the Influence of Pyocyanine on the Gas Exchange of a Pyocyanine-Free Strain of P. aerugino","volume":"47","author":"Lichstein","year":"1944","journal-title":"J. Bacteriol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1128\/aem.59.4.1114-1119.1993","article-title":"Growth of syntrophic propionate-oxidizing bacteria with fumarate in the absence of methanogenic bacteria","volume":"59","author":"Stams","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Silva, R.M., Fernandes, A.M., Fiume, F., Castro, A.R., Machado, R., and Pereira, M.A. (2021). Sequencing batch airlift reactors (SBAR): A suitable technology for treatment and valorization of mineral oil wastewaters towards lipids production. J. Hazard. Mater., 409.","DOI":"10.1016\/j.jhazmat.2020.124492"},{"key":"ref_41","unstructured":"Platen, P.H., and Wirtz, A. (1999). Applications of Analysis Measurement of the Respiration Activity of Soils Using the OxiTop Control Measuring System Basic Principles and Process Characteristic Quantities, WTW. Available online: https:\/\/download.sechang.com\/pds\/2000\/2000_18033a.pdf."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1080\/01490451.2011.619633","article-title":"Bacterial Diversity and Geochemical Profiles in Sediments from Eutrophic Azorean Lakes","volume":"29","author":"Martins","year":"2012","journal-title":"Geomicrobiol. J."},{"key":"ref_43","unstructured":"APHA, AWWA, and WPCF (1999). Standard Methods for the Examination of Water and Wastewater, American Public Health Association."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"725","DOI":"10.4141\/S05-075","article-title":"A proposed method for rapid and economical extraction of petroleum hydrocarbons from contaminated soils","volume":"86","author":"Siddique","year":"2006","journal-title":"Can. J. Soil Sci."},{"key":"ref_45","unstructured":"EPA (1996). Method 3510C: Separatory Funnel Liquid-Liquid Extraction, United States Environmental Protection Agency."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1111\/1751-7915.12886","article-title":"Enhancement of methane production from 1-hexadecene by additional electron donors","volume":"11","author":"Paulo","year":"2018","journal-title":"Microb. Biotechnol."},{"key":"ref_47","first-page":"831","article-title":"Oxygen dynamics in petroleum hydrocarbon contaminated salt marsh soils: III","volume":"24","author":"Shin","year":"2003","journal-title":"A rate model. Environ. Technol."},{"key":"ref_48","unstructured":"EPA (2022, March 17). CompTox Chemicals Dashboard, Available online: https:\/\/comptox.epa.gov\/dashboard\/."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Sparks, D.L. (2003). 5\u2014Sorption Phenomena on Soils. Environmental Soil Chemistry, Academic Press. [2nd ed.].","DOI":"10.1016\/B978-012656446-4\/50005-0"},{"key":"ref_50","unstructured":"Vilarinho, C., Castro, F., Gon\u00e7alves, M., and Fernando, A.L. (2020). Microbial conversion of oily wastes to methane: Effect of ferric nanomaterials. Wastes: Solutions, Treatments and Opportunities III, Proceedings of the 5th International Conference Wastes 2019, Lisbon, Portugal, 4\u20136 September 2019, Taylor & Francis Group."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.jhazmat.2007.05.063","article-title":"Application of biosurfactants, rhamnolipid, and surfactin, for enhanced biodegradation of diesel-contaminated water and soil","volume":"151","author":"Whang","year":"2008","journal-title":"J. Hazard. Mater."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1574-6941.2000.tb00694.x","article-title":"Hexadecane mineralization and denitrification in two diesel fuel-contaminated soils","volume":"32","author":"Roy","year":"2000","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1002\/etc.5620170705","article-title":"Bioavailability and biodegradation of weathered diesel fuel in aquifer material under denitrifying conditions","volume":"17","author":"Bregnard","year":"1998","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1021\/es9500872","article-title":"Model for microbial degradation of nonpolar organic contaminants in a soil slurry reactor","volume":"30","author":"Geerdink","year":"1996","journal-title":"Environ. Sci. Technol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5892","DOI":"10.1021\/es200649t","article-title":"Anaerobic Biodegradation of Longer-Chain n-Alkanes Coupled to Methane Production in Oil Sands Tailings","volume":"45","author":"Siddique","year":"2011","journal-title":"Environ. Sci. Technol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1126\/science.1063294","article-title":"Anaerobes to the Rescue","volume":"293","author":"Lovley","year":"2001","journal-title":"Science"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1038\/35008145","article-title":"Hexadecane decay by methanogenesis","volume":"404","author":"Anderson","year":"2000","journal-title":"Nature"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Sherry, A., Grant, R.J., Aitken, C.M., Jones, M., Bowler, B.F.J., Larter, S.R., Head, I.M., and Gray, N.D. (2020). Methanogenic crude oil-degrading microbial consortia are not universally abundant in anoxic environments. Int. Biodeterior. Biodegrad., 155.","DOI":"10.1016\/j.ibiod.2020.105085"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1002\/bit.1172","article-title":"Mass transfer effects on microbial uptake of naphthalene from complex NAPLs","volume":"75","author":"Mukherji","year":"2001","journal-title":"Biotechnol. Bioeng."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Hamdi, W., Gamaoun, F., Pelster, D.E., and Seffen, M. (2013). Nitrate sorption in an agricultural soil profile. Appl. Environ. Soil Sci., 2013.","DOI":"10.1155\/2013\/597824"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0016-7061(03)00186-1","article-title":"Sulfate adsorption and its relationships with properties of representative soils of the S\u00e3o Paulo State, Brazil","volume":"118","author":"Alves","year":"2004","journal-title":"Geoderma"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1080\/10889869991219433","article-title":"Biodegradation rates for fuel Hydrocarbons and Chlorinated Solvents in groundwater","volume":"3","author":"Suarez","year":"1999","journal-title":"Bioremediat. J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1186\/s13068-017-0895-9","article-title":"Coexistence and competition of sulfate-reducing and methanogenic populations in an anaerobic hexadecane-degrading culture","volume":"10","author":"Ma","year":"2017","journal-title":"Biotechnol. Biofuels"}],"container-title":["Applied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3417\/12\/21\/11069\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:08:43Z","timestamp":1760144923000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3417\/12\/21\/11069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,1]]},"references-count":63,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["app122111069"],"URL":"https:\/\/doi.org\/10.3390\/app122111069","relation":{},"ISSN":["2076-3417"],"issn-type":[{"value":"2076-3417","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,1]]}}}