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P2020","award":["POCI-01-0247-FEDER-035234"],"award-info":[{"award-number":["POCI-01-0247-FEDER-035234"]}]},{"name":"Portuguese national budget P2020","award":["LISBOA-01-0247-FEDER-035234"],"award-info":[{"award-number":["LISBOA-01-0247-FEDER-035234"]}]},{"name":"Portuguese national budget P2020","award":["ALG-01-0247-FEDER-035234"],"award-info":[{"award-number":["ALG-01-0247-FEDER-035234"]}]},{"name":"Portuguese national budget P2020","award":["ALG-01-0247-FEDER-069961"],"award-info":[{"award-number":["ALG-01-0247-FEDER-069961"]}]},{"name":"Portuguese national budget P2020","award":["023310\u2013ALGACO2"],"award-info":[{"award-number":["023310\u2013ALGACO2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>The nutritional requirements of novel microalgal strains are key for their effective cultivation and metabolite content. Therefore, the optimization of heterotrophic and photoautotrophic culture media is crucial for novel Chlorococcum amblystomatis growth. Heterotrophic and photoautotrophic biomass samples were characterized to identify the differences between their heterotrophic and photoautotrophic biomass composition and their biotechnological potential. Media optimization through surface response methodology led to 44.9 and 51.2% increments in C. amblystomatis-specific growth rates under heterotrophic and photoautotrophic growth, respectively. This microalga registered high protein content (61.49\u201373.45% dry weight), with the highest value being observed in the optimized photoautotrophic growth medium. The lipid fraction mainly constituted polyunsaturated fatty acids, ranging from 44.47 to 51.41% for total fatty acids (TFA) in cells under heterotrophy. However, these contents became significantly higher (70.46\u201372.82% TFA) in cultures cultivated under photoautotrophy. An interesting carotenoids content was achieved in the cultures grown in optimized photoautotrophic medium: 5.84 mg\u00b7g\u22121\u00a0\u03b2-carotene, 5.27 mg\u00b7g\u22121 lutein, 3.66 mg\u00b7g\u22121 neoxanthin, and 0.75 mg\u00b7g\u22121 violaxanthin. Therefore, C. amblystomatis demonstrated an interesting growth performance and nutritional profile for food supplements and feed products that might contribute to meeting the world\u2019s nutritional demand.<\/jats:p>","DOI":"10.3390\/app13042089","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T06:22:25Z","timestamp":1675664545000},"page":"2089","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Heterotrophic and Photoautotrophic Media Optimization Using Response Surface Methodology for the Novel Microalga Chlorococcum amblystomatis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5411-8686","authenticated-orcid":false,"given":"N\u00e1dia","family":"Correia","sequence":"first","affiliation":[{"name":"CCMAR\u2014Centro de Ci\u00eancias do Mar, Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal"},{"name":"Allmicroalgae Natural Products S.A., Research and Development Department-Rua 25 de Abril, 2445-413 Alcoba\u00e7a, Portugal"},{"name":"LNEG\u2014Laborat\u00f3rio Nacional de Energia e Geologia I.P., Bioenergy and Biorefineries Department, Estrada do Pa\u00e7o do Lumiar 22, 1649-038 Lisbon, Portugal"}]},{"given":"Hugo","family":"Pereira","sequence":"additional","affiliation":[{"name":"GreenCoLab-Associa\u00e7\u00e3o Oceano Verde, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"given":"Peter S. C.","family":"Schulze","sequence":"additional","affiliation":[{"name":"GreenCoLab-Associa\u00e7\u00e3o Oceano Verde, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"given":"Monya M.","family":"Costa","sequence":"additional","affiliation":[{"name":"GreenCoLab-Associa\u00e7\u00e3o Oceano Verde, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3924-2256","authenticated-orcid":false,"given":"Gon\u00e7alo E.","family":"Santo","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department-Rua 25 de Abril, 2445-413 Alcoba\u00e7a, Portugal"}]},{"given":"In\u00eas","family":"Guerra","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department-Rua 25 de Abril, 2445-413 Alcoba\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9465-7681","authenticated-orcid":false,"given":"Mafalda","family":"Trov\u00e3o","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department-Rua 25 de Abril, 2445-413 Alcoba\u00e7a, Portugal"},{"name":"GreenCoLab-Associa\u00e7\u00e3o Oceano Verde, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"given":"Ana","family":"Barros","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department-Rua 25 de Abril, 2445-413 Alcoba\u00e7a, Portugal"}]},{"given":"Helena","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department-Rua 25 de Abril, 2445-413 Alcoba\u00e7a, Portugal"}]},{"given":"Joana L.","family":"Silva","sequence":"additional","affiliation":[{"name":"Allmicroalgae Natural Products S.A., Research and Development Department-Rua 25 de Abril, 2445-413 Alcoba\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0196-6360","authenticated-orcid":false,"given":"Lu\u00edsa","family":"Gouveia","sequence":"additional","affiliation":[{"name":"LNEG\u2014Laborat\u00f3rio Nacional de Energia e Geologia I.P., Bioenergy and Biorefineries Department, Estrada do Pa\u00e7o do Lumiar 22, 1649-038 Lisbon, Portugal"},{"name":"GreenCoLab-Associa\u00e7\u00e3o Oceano Verde, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3101-693X","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Varela","sequence":"additional","affiliation":[{"name":"CCMAR\u2014Centro de Ci\u00eancias do Mar, Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal"},{"name":"GreenCoLab-Associa\u00e7\u00e3o Oceano Verde, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1007\/s00253-004-1647-x","article-title":"Valuable products from biotechnology of microalgae","volume":"65","author":"Pulz","year":"2004","journal-title":"Appl. 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