{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T20:42:15Z","timestamp":1776199335998,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T00:00:00Z","timestamp":1690416000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"National Funds through the FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/4255\/2020"],"award-info":[{"award-number":["UIDP\/4255\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>This investigation aimed to assess, through a systematic review, the effect of non-thermal plasma treatments on root canal sealers\u2019 adhesion to dentin. This study followed the 2020 Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. A literature search was undertaken without limits on time or language, until May 2023, in PubMed\u2013MEDLINE, Scopus, Web of Science, OpenGrey, and three endodontic journals. The included studies underwent quality assessment and data extraction. Out of an initial 188 articles, 4 studies were included. Three of these studies based the adhesion ability on the push-out test in human extracted teeth, while the other used bovine dentin samples to measure the contact angle with the sealer (wettability). While there was no consensus about the effect of non-thermal plasma (NTP) on the AH Plus sealer\u2019s adhesion to radicular dentin, NTP seemed to positively influence the adhesion ability of BioRoot RCS and Endosequence BC. The findings of the present review should be interpreted cautiously due to the scarcity of studies on the topic. The NTP parameters should be optimized to obtain a stronger evidence base in endodontics on its role as an adjuvant tool to increase sealers\u2019 adhesion to dentin.<\/jats:p>","DOI":"10.3390\/app13158655","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T02:10:45Z","timestamp":1690510245000},"page":"8655","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of Plasma Treatment on Root Canal Sealers\u2019 Adhesion to Intraradicular Dentin\u2014A Systematic Review"],"prefix":"10.3390","volume":"13","author":[{"given":"In\u00eas","family":"Ferreira","sequence":"first","affiliation":[{"name":"School of Medicine and Dentistry, University of Santiago de Compostela, 15705 Santiago de Compostela, Spain"},{"name":"CINTESIS, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8266-0111","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Lopes","sequence":"additional","affiliation":[{"name":"Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, 4710-057 Braga, Portugal"},{"name":"LaPMET\u2014Laboratory of Physics for Materials and Emergent Technologies, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5294-0112","authenticated-orcid":false,"given":"Armando","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, 4710-057 Braga, Portugal"},{"name":"LaPMET\u2014Laboratory of Physics for Materials and Emergent Technologies, University of Minho, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5506-996X","authenticated-orcid":false,"given":"Filipe","family":"Vaz","sequence":"additional","affiliation":[{"name":"Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, 4710-057 Braga, Portugal"},{"name":"LaPMET\u2014Laboratory of Physics for Materials and Emergent Technologies, University of Minho, 4710-057 Braga, Portugal"}]},{"given":"Irene","family":"Pina-Vaz","sequence":"additional","affiliation":[{"name":"CINTESIS@RISE, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4297-4220","authenticated-orcid":false,"given":"Benjam\u00edn","family":"Mart\u00edn-Biedma","sequence":"additional","affiliation":[{"name":"Oral Sciences Research Group, Endodontics and Restorative Dentistry Unit, School of Medicine and Dentistry, Health Research Institute of Santiago (IDIS), University of Santiago de Compostela, 15705 Santiago de Compostela, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"European Society of Endodontology (2006). Quality guidelines for endodontic treatment: Consensus report of the European Society of Endodontology. Int. Endod. J., 39, 921\u2013930.","DOI":"10.1111\/j.1365-2591.2006.01180.x"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"369","DOI":"10.4103\/jcd.jcd_142_21","article-title":"A novel modified obturation technique using biosealers: An ex vivo study","volume":"24","author":"Abdellatif","year":"2021","journal-title":"J. Conserv. Dent."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/j.joen.2006.08.003","article-title":"Adhesive dentistry and endodontics. Part 2: Bonding in the root canal system-the promise and the problems: A review","volume":"32","author":"Schwartz","year":"2006","journal-title":"J. Endod."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"703","DOI":"10.4012\/dmj.2019-288","article-title":"Comprehensive review of current endodontic sealers","volume":"39","author":"Komabayashi","year":"2020","journal-title":"Dent. Mater. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4666","DOI":"10.1038\/s41598-021-83849-y","article-title":"A matched irrigation and obturation strategy for root canal therapy","volume":"11","author":"Hadis","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.jebdp.2019.04.004","article-title":"Dislodgment Resistance of Bioceramic and Epoxy Sealers: A Systematic Review and Meta-analysis","volume":"19","author":"Silva","year":"2019","journal-title":"J. Evid.-Based Dent. Pract."},{"key":"ref_7","first-page":"71","article-title":"Adhesion and formation of tags from MTA Fillapex compared with AH Plus\u00ae cement","volume":"11","author":"BaechtoldI","year":"2014","journal-title":"RSBO"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.joen.2012.06.030","article-title":"Evaluation of cytotoxicity and physicochemical properties of calcium silicate-based endodontic sealer MTA Fillapex","volume":"39","author":"Silva","year":"2013","journal-title":"J. Endod."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1111\/j.1365-2591.2011.01925.x","article-title":"Push-out bond strength of two new calcium silicate-based endodontic sealers to root canal dentine","volume":"44","author":"Sagsen","year":"2011","journal-title":"Int. Endod. J."},{"key":"ref_10","first-page":"9753210","article-title":"Bioceramic-Based Root Canal Sealers: A Review","volume":"2016","year":"2016","journal-title":"Int. J. Biomater."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.joen.2016.11.019","article-title":"Are Premixed Calcium Silicate-based Endodontic Sealers Comparable to Conventional Materials? A Systematic Review of In Vitro Studies","volume":"43","author":"Moraes","year":"2017","journal-title":"J. Endod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100200","DOI":"10.1016\/j.mtbio.2021.100200","article-title":"Aurora Borealis in dentistry: The applications of cold plasma in biomedicine","volume":"13","author":"Lata","year":"2022","journal-title":"Mater. Today Bio"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1590\/0103-6440201600486","article-title":"Effect of Argon Plasma on Root Dentin after Use of 6% NaOCl","volume":"27","author":"Prado","year":"2016","journal-title":"Braz. Dent. J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1111\/j.1600-0722.2010.00761.x","article-title":"Dentin surface treatment using a non-thermal argon plasma brush for interfacial bonding improvement in composite restoration","volume":"118","author":"Ritts","year":"2010","journal-title":"Eur. J. Oral Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1016\/j.dental.2013.05.002","article-title":"Surface modification of several dental substrates by non-thermal, atmospheric plasma brush","volume":"29","author":"Chen","year":"2013","journal-title":"Dent. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jhin.2003.09.019","article-title":"Plasma cleaning of dental instruments","volume":"56","author":"Whittaker","year":"2004","journal-title":"J. Hosp. Infect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1590\/1679-775720130016","article-title":"High-efficiency tooth bleaching using non-thermal atmospheric pressure plasma with low concentration of hydrogen peroxide","volume":"21","author":"Nam","year":"2013","journal-title":"J. Appl. Oral Sci. Rev. FOB"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Jungbauer, G., Moser, D., M\u00fcller, S., Pfister, W., Sculean, A., and Eick, S. (2021). The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens-A Systematic Review of In-Vitro Studies. Antibiotics, 10.","DOI":"10.3390\/antibiotics10020211"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1002\/ppap.201200088","article-title":"Modification of Enamel and Dentin Surfaces by Non-Thermal Atmospheric Plasma","volume":"10","author":"Lehmann","year":"2013","journal-title":"Plasma Process. Polym."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/j.jclinepi.2009.06.005","article-title":"Preferred reporting items for systematic reviews and meta-analyses: The PRISMA statement","volume":"62","author":"Moher","year":"2009","journal-title":"J. Clin. Epidemiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1111\/aej.12563","article-title":"Influence of the use of chelating agents as final irrigant on the push-out bond strength of epoxy resin-based root canal sealers: A systematic review","volume":"48","author":"Augusto","year":"2022","journal-title":"Aust. Endod. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.msec.2016.05.062","article-title":"Surface modification of gutta-percha cones by non-thermal plasma","volume":"68","author":"Prado","year":"2016","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"811","DOI":"10.4103\/njcp.njcp_578_19","article-title":"The effect of nonthermal plasma on the push-out bond strength of two different root canal sealers","volume":"23","author":"Yeter","year":"2020","journal-title":"Niger. J. Clin. Pract."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1590\/1678-7757-2016-0498","article-title":"Effect of photodynamic therapy and non-thermal plasma on root canal filling: Analysis of adhesion and sealer penetration","volume":"25","author":"Menezes","year":"2017","journal-title":"J. Appl. Oral Sci. Rev. FOB"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"41","DOI":"10.4103\/JCD.JCD_500_20","article-title":"Effect of nonthermal atmospheric plasma on the push-out bond strength of epoxy resin-based and bioceramic root canal sealers: An in vitro study","volume":"24","author":"Garlapati","year":"2021","journal-title":"J. Conserv. Dent. JCD"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1016\/j.joen.2018.05.003","article-title":"Design Variability of the Push-out Bond Test in Endodontic Research: A Systematic Review","volume":"44","author":"Brichko","year":"2018","journal-title":"J. Endod."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1111\/iej.13085","article-title":"Bovine teeth can reliably substitute human dentine in an intra-tooth push-out bond strength model?","volume":"52","author":"Silva","year":"2019","journal-title":"Int. Endod. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1111\/j.1365-2591.2010.01848.x","article-title":"The impact of root dentine conditioning on sealing ability and push-out bond strength of an epoxy resin root canal sealer","volume":"44","author":"Neelakantan","year":"2011","journal-title":"Int. Endod. J."},{"key":"ref_29","first-page":"17","article-title":"Effect of Gutta-percha Solvents on the Bond Strength of Sealers to Intraradicular Dentin: A Systematic Review","volume":"16","author":"Ferreira","year":"2021","journal-title":"Iran. Endod. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.joen.2011.03.021","article-title":"Effect of disinfectant solutions on the surface free energy and wettability of filling material","volume":"37","author":"Prado","year":"2011","journal-title":"J. Endod."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"124","DOI":"10.4103\/0972-0707.153075","article-title":"Evaluation of push-out bond strength of endosequence BC sealer with lateral condensation and thermoplasticized technique: An in vitro study","volume":"18","author":"Gade","year":"2015","journal-title":"J. Conserv. Dent."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1016\/j.joen.2011.08.014","article-title":"Evaluation of the interaction between endodontic sealers and dentin treated with different irrigant solutions","volume":"37","author":"Prado","year":"2011","journal-title":"J. Endod."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1016\/j.joen.2011.06.005","article-title":"Quantitative analysis of the effect of irrigant solution sequences on dentin erosion","volume":"37","author":"Qian","year":"2011","journal-title":"J. Endod."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.cpme.2014.04.002","article-title":"Plasma in dentistry","volume":"2","author":"Cha","year":"2014","journal-title":"Clin. Plasma Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5117","DOI":"10.1007\/s00253-019-09877-x","article-title":"Technical applications of plasma treatments: Current state and perspectives","volume":"103","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_36","first-page":"83","article-title":"Atmospheric pressure glow discharge plasma and its applications in textile","volume":"31","author":"Samanta","year":"2006","journal-title":"Indian J. Fibre Text. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/00016357.2013.795660","article-title":"Plasma in dentistry: A review of basic concepts and applications in dentistry","volume":"72","author":"Kim","year":"2014","journal-title":"Acta Odontol. Scand."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Borges, A.C., Kostov, K.G., Pessoa, R.S., de Abreu, G.M.A., Lima, G.d.M.G., Figueira, L.W., and Koga-Ito, C.Y. (2021). Applications of Cold Atmospheric Pressure Plasma in Dentistry. Appl. Sci., 11.","DOI":"10.3390\/app11051975"},{"key":"ref_39","first-page":"e15","article-title":"The impact of different low-pressure plasma types on the physical, chemical and biological surface properties of PEEK","volume":"37","author":"Fu","year":"2021","journal-title":"Dent. Mater. Off. Publ. Acad. Dent. Mater."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1590\/0103-6440201600914","article-title":"Effect of non-thermal argon plasma on bond strength of a self-etch adhesive system to NaOCl-Treated Dentin","volume":"27","author":"Abreu","year":"2016","journal-title":"Braz. Dent. J."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sevilla, P., Lopez-Suarez, C., Pelaez, J., Tobar, C., Rodriguez-Alonso, V., and Suarez, M.J. (2020). Influence of low-pressure plasma on the surface properties of CAD-CAM leucite-reinforced feldspar and resin matrix ceramics. Appl. Sci., 10.","DOI":"10.3390\/app10248856"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"104762","DOI":"10.1016\/j.jmbbm.2021.104762","article-title":"In vitro study on how cold plasma affects dentin surface characteristics","volume":"123","author":"Suzuki","year":"2021","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Izdebska-Podsiad\u0142y, J. (2023). Study of Argon and Oxygen Mixtures in Low Temperature Plasma for Improving PLA Film Wettability. Coatings, 13.","DOI":"10.3390\/coatings13020279"}],"container-title":["Applied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3417\/13\/15\/8655\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:20:34Z","timestamp":1760127634000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3417\/13\/15\/8655"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,27]]},"references-count":43,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["app13158655"],"URL":"https:\/\/doi.org\/10.3390\/app13158655","relation":{},"ISSN":["2076-3417"],"issn-type":[{"value":"2076-3417","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,27]]}}}