{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T11:52:32Z","timestamp":1769860352352,"version":"3.49.0"},"reference-count":41,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T00:00:00Z","timestamp":1696291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT","award":["UID\/BIM\/04293\/2013"],"award-info":[{"award-number":["UID\/BIM\/04293\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>Gold nanoparticles (AuNPs) have garnered attention as a potential alternative to conventional antibiotics due to their innovative antibacterial properties. This study demonstrates the successful production of biosynthetic gold nanoparticles (bAuNPs) using Pseudomonas aeruginosa (P. aeruginosa) as spherical nanostructures at 58 \u00b0C for 24 h, under alkaline pH (9.0). The successful synthesis of bAuNPs was confirmed through UV-Vis spectroscopy, exhibiting a characteristic peak within the 500\u2013600 nm wavelength range, and the evaluation of the main functional groups and morphology were stressed by Fourier Transform Infrared Spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM), respectively. Subsequently, the synthesized bAuNPs were combined with low concentrations of ampicillin and evaluated against Methicillin-Susceptible Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) through the classical serial dilution method. This innovative approach holds the potential to address the escalating issue of antibiotic resistance, providing a viable and sustainable solution.<\/jats:p>","DOI":"10.3390\/app131910934","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T01:48:17Z","timestamp":1696297697000},"page":"10934","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Magic Cocktail: Ampicillin and Biosynthesized Gold Nanoparticles Synergism against Staphylococcus\u00a0aureus"],"prefix":"10.3390","volume":"13","author":[{"given":"Marco","family":"Oliveira","sequence":"first","affiliation":[{"name":"FP-BHS\u2014Biomedical and Health Sciences Research Unit, FP-I3ID\u2014Instituto de Investiga\u00e7\u00e3o, Inova\u00e7\u00e3o e Desenvolvimento, FFP\u2014Funda\u00e7\u00e3o Fernando Pessoa, 4420-096 Porto, Portugal"},{"name":"CTBIO\u2014Polytechnic, Institute of Porto, 4420-096 Porto, Portugal"},{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4344-7860","authenticated-orcid":false,"given":"Silvia","family":"Soares","sequence":"additional","affiliation":[{"name":"FP-BHS\u2014Biomedical and Health Sciences Research Unit, FP-I3ID\u2014Instituto de Investiga\u00e7\u00e3o, Inova\u00e7\u00e3o e Desenvolvimento, FFP\u2014Funda\u00e7\u00e3o Fernando Pessoa, 4420-096 Porto, Portugal"},{"name":"HE-FP, Hospital Fernando Pessoa, CECLIN\u2014Center of Clinical Studies, 4420-096 Gondomar, Portugal"},{"name":"Faculty of Medicine, University of Porto, 4420-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1376-2624","authenticated-orcid":false,"given":"Sara","family":"S\u00e1","sequence":"additional","affiliation":[{"name":"FP-BHS\u2014Biomedical and Health Sciences Research Unit, FP-I3ID\u2014Instituto de Investiga\u00e7\u00e3o, Inova\u00e7\u00e3o e Desenvolvimento, FFP\u2014Funda\u00e7\u00e3o Fernando Pessoa, 4420-096 Porto, Portugal"},{"name":"HE-FP, Hospital Fernando Pessoa, CECLIN\u2014Center of Clinical Studies, 4420-096 Gondomar, Portugal"},{"name":"Faculty of Medicine, University of Porto, 4420-319 Porto, Portugal"}]},{"given":"\u00c1lvaro","family":"Gestoso","sequence":"additional","affiliation":[{"name":"FP-BHS\u2014Biomedical and Health Sciences Research Unit, FP-I3ID\u2014Instituto de Investiga\u00e7\u00e3o, Inova\u00e7\u00e3o e Desenvolvimento, FFP\u2014Funda\u00e7\u00e3o Fernando Pessoa, 4420-096 Porto, Portugal"},{"name":"HE-FP, Hospital Fernando Pessoa, CECLIN\u2014Center of Clinical Studies, 4420-096 Gondomar, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1950-1414","authenticated-orcid":false,"given":"Miguel","family":"Correa-Duarte","sequence":"additional","affiliation":[{"name":"CINBIO\u2014Center for Research in Nanomaterials and Biomedicine, University of Vigo, 36310 Vigo, Spain"},{"name":"Faculty of Chemistry, Department of Physical Chemistry, University of Vigo, 36310 Vigo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3740-862X","authenticated-orcid":false,"given":"Pilar","family":"Baylina","sequence":"additional","affiliation":[{"name":"FP-BHS\u2014Biomedical and Health Sciences Research Unit, FP-I3ID\u2014Instituto de Investiga\u00e7\u00e3o, Inova\u00e7\u00e3o e Desenvolvimento, FFP\u2014Funda\u00e7\u00e3o Fernando Pessoa, 4420-096 Porto, Portugal"},{"name":"HE-FP, Hospital Fernando Pessoa, CECLIN\u2014Center of Clinical Studies, 4420-096 Gondomar, Portugal"},{"name":"School of Health of the Polytechnic, Institute of Porto, 4420-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8933-3984","authenticated-orcid":false,"given":"R\u00faben","family":"Fernandes","sequence":"additional","affiliation":[{"name":"FP-BHS\u2014Biomedical and Health Sciences Research Unit, FP-I3ID\u2014Instituto de Investiga\u00e7\u00e3o, Inova\u00e7\u00e3o e Desenvolvimento, FFP\u2014Funda\u00e7\u00e3o Fernando Pessoa, 4420-096 Porto, Portugal"},{"name":"HE-FP, Hospital Fernando Pessoa, CECLIN\u2014Center of Clinical Studies, 4420-096 Gondomar, Portugal"},{"name":"Faculty of Health Sciences, Fernando Pessoa University, 420-150 Porto, Portugal"}]},{"given":"Carla F.","family":"Pereira","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1111\/1751-7915.12884","article-title":"The race for antimicrobials in the multidrug resistance era","volume":"11","author":"Romero","year":"2018","journal-title":"Microb. 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