{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T07:06:57Z","timestamp":1777100817619,"version":"3.51.4"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"9031-2020-BG-CONENTPRIV-FONDCRUI-GOFORIT_001"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>Seed priming with synthetic chemicals may be harmful to the environment and human health. Their replacement with bio-based compounds may overcome these concerns. In this study, we assessed the effectiveness of wood distillate (WD) in enhancing in vitro germination of crop plants using basil, chickpea, and lettuce as case studies. Seeds of the three species were soaked for 24 h in 0.25% and 0.17% WD solutions and then left to germinate for 7 days at 20 \u00b0C in a dark germination chamber. Seed pre-treatment with 0.25% WD enhanced germination in all tested species, while 0.17% WD stimulated germination in lettuce and chickpea, but not in basil. For lettuce, 0.17% WD worked better than 0.25% WD. Radicle length of basil and chickpea increased following pre-treatment with 0.25% WD, while in lettuce, it increased after pre-treatment with 0.17% WD. Treating seeds with appropriate WD solutions is a potential strategy to improve germination of crop plants.<\/jats:p>","DOI":"10.3390\/app14020631","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T08:27:07Z","timestamp":1704961627000},"page":"631","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Wood Distillate Enhances Seed Germination of Chickpea, Lettuce, and Basil"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4656-320X","authenticated-orcid":false,"given":"Viviana","family":"Maresca","sequence":"first","affiliation":[{"name":"Department of Life Sciences, University of Siena, 53100 Siena, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8736-1313","authenticated-orcid":false,"given":"Riccardo","family":"Fedeli","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, 53100 Siena, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3789-6691","authenticated-orcid":false,"given":"Andrea","family":"Vannini","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, 53100 Siena, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0071-7699","authenticated-orcid":false,"given":"Silvana","family":"Munzi","sequence":"additional","affiliation":[{"name":"Centro Interuniversit\u00e1rio de Hist\u00f3ria das Ci\u00eancias e da Tecnologia Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"},{"name":"Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"given":"Ana","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3100-463X","authenticated-orcid":false,"given":"Cristina","family":"Cruz","sequence":"additional","affiliation":[{"name":"Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3404-1017","authenticated-orcid":false,"given":"Stefano","family":"Loppi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, 53100 Siena, Italy"},{"name":"BAT Center\u2014Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples \u2018Federico II\u2019, 80138 Napoli, Italy"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,11]]},"reference":[{"key":"ref_1","first-page":"1249","article-title":"Effect of osmopriming with polyethylene glycol 8000 (PEG8000) on germination and seedling growth of wheat (Triticum aestivum L.) seeds under salt stress","volume":"3","author":"Ghiyasi","year":"2008","journal-title":"Res. J. Biol. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"623","DOI":"10.15258\/sst.2005.33.3.09","article-title":"Physiological and Biochemical Aspects of Seed Vigour Enhancement Treatments in Fine Rice (Oryza sativa L.)","volume":"33","author":"Basra","year":"2005","journal-title":"J. Seed Sci. Technol."},{"key":"ref_3","unstructured":"Broud, K.P. (2006). Mineral Nutrition of Higher Plant, Academic Press. [2nd ed.]."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1007\/s00299-015-1784-y","article-title":"Seed priming: State of the art and new perspectives","volume":"34","author":"Paparella","year":"2015","journal-title":"Plant Cell Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"10301","DOI":"10.1038\/s41598-018-28712-3","article-title":"Arsenic\u2013silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes","volume":"8","author":"Khan","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4311","DOI":"10.1038\/s41598-019-40849-3","article-title":"Rice seed priming with sodium selenate: Effects on germination, seedling growth, and biochemical attributes","volume":"9","author":"Du","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jhazmat.2018.05.017","article-title":"Rice seed priming with Se: A novel approach to mitigate as induced adverse consequences on growth, yield and as load in brown rice","volume":"355","author":"Moulick","year":"2018","journal-title":"J. Hazard. Mater."},{"key":"ref_8","first-page":"172","article-title":"Use of commercial fertilizers as osmotica for rice priming","volume":"1","author":"Farooq","year":"2005","journal-title":"J. Agric. Soc. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.jplph.2013.12.007","article-title":"Abscisic acid and abiotic stress tolerance\u2013Different tiers of regulation","volume":"171","author":"Mehrotra","year":"2014","journal-title":"J. Plant Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.jaap.2018.09.008","article-title":"Production, prospects and potential application of pyroligneous acid in agriculture","volume":"135","author":"Grewal","year":"2018","journal-title":"J. Anal. Appl. Pyrolysis"},{"key":"ref_11","first-page":"1508","article-title":"Effect of pyroligneous acid on growth, yield and quality improvement of rockmelon in soilless culture","volume":"5","author":"Zulkarami","year":"2011","journal-title":"Aust. J. Crop Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1007\/s10086-002-0472-z","article-title":"Effect of bamboo vinegar on regulation of germination and radicle growth of seed plants","volume":"49","author":"Mu","year":"2003","journal-title":"J. Wood Sci."},{"key":"ref_13","unstructured":"(2022, October 12). Italian Ministerial Decree 6793. Available online: https:\/\/www.gazzettaufficiale.it\/eli\/id\/2018\/09\/05\/18A05693\/sg."},{"key":"ref_14","first-page":"012186","article-title":"Effect of adding wood vinegar on cucumber (Cucumis sativus L.) seed germination","volume":"Volume 128","author":"Lei","year":"2018","journal-title":"IOP Conference Series: Earth and Environmental Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/aab.12794","article-title":"Foliar application of wood distillate boosts plant yield and nutritional parameters of chickpea","volume":"182","author":"Fedeli","year":"2023","journal-title":"Ann. Appl. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Fedeli, R., Vannini, A., Guarnieri, M., Monaci, F., and Loppi, S. (2022). Bio-Based Solutions for Agriculture: Foliar Application of Wood Distillate Alone and in Combination with Other Plant-Derived Corroborants Results in Different Effects on Lettuce (Lactuca Sativa L.). Biology, 11.","DOI":"10.3390\/biology11030404"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ofoe, R., Qin, D., Gunupuru, L.R., Thomas, R.H., and Abbey, L. (2022). Effect of pyroligneous acid on the productivity and nutritional quality of greenhouse tomato. Plants, 11.","DOI":"10.2139\/ssrn.4027336"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/aab.12837","article-title":"Wood distillate (pyroligneous acid) boosts nutritional traits of potato tubers","volume":"183","author":"Fedeli","year":"2023","journal-title":"Ann. Appl. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5324","DOI":"10.1111\/1750-3841.16829","article-title":"Nutritionally enriched tomatoes (Solanum lycopersicum L.) grown with wood distillate: Chemical and biological characterization for quality assessment","volume":"88","author":"Fedeli","year":"2023","journal-title":"J. Food Sci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Filippelli, A., Ciccone, V., Loppi, S., and Morbidelli, L. (2021). Characterization of the safety profile of sweet chestnut wood distillate employed in agriculture. Safety, 7.","DOI":"10.3390\/safety7040079"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"24","DOI":"10.2137\/145960610791015023","article-title":"Acute toxicity of birch tar oil on aquatic organisms","volume":"19","author":"Hagner","year":"2010","journal-title":"Agric. Food Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13","DOI":"10.2137\/145960610791015096","article-title":"Effects of birch tar oils on soil organisms and plants","volume":"19","author":"Hagner","year":"2010","journal-title":"Agric. Food Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Fa\u010dkovcov\u00e1, Z., Vannini, A., Monaci, F., Grattacaso, M., Paoli, L., and Loppi, S. (2020). Uptake of trace elements in the water fern Azolla filiculoides after short-term application of chestnut wood distillate (Pyroligneous acid). Plants, 9.","DOI":"10.3390\/plants9091179"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Fanfarillo, E., Fedeli, R., Fiaschi, T., de Simone, L., Vannini, A., Angiolini, C., and Maccherini, S. (2022). Effects of wood distillate on seedling emergence and first-stage growth in five threatened arable plants. Diversity, 14.","DOI":"10.3390\/d14080669"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.jfca.2016.03.004","article-title":"Nutritional value, bioactive compounds and health benefits of lettuce (Lactuca sativa L.)","volume":"49","author":"Kim","year":"2016","journal-title":"J. Food Compos. Anal."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.clnu.2003.10.009","article-title":"Health effect of vegetable-based diet: Lettuce consumption improves cholesterol metabolism and antioxidant status in the rat","volume":"23","author":"Nicolle","year":"2004","journal-title":"Clin. Nutr."},{"key":"ref_27","first-page":"499","article-title":"Basil: A source of aroma compounds and a popular culinary and ornamental herb","volume":"16","author":"Simon","year":"1999","journal-title":"Perspect. New Crops New Uses"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1300\/J044v13n03_10","article-title":"Ocimum sp.(basil): Botany, cultivation, pharmaceutical properties, and biotechnology","volume":"13","author":"Makri","year":"2008","journal-title":"J. Herbs Spices Med. Plants"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1017\/S0007114512000797","article-title":"Nutritional quality and health benefits of chickpea (Cicer arietinum L.): A review","volume":"108","author":"Jukanti","year":"2012","journal-title":"Br. J. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0378-4290(97)00029-4","article-title":"Chickpea (Cicer arietinum L.)","volume":"53","author":"Singh","year":"1997","journal-title":"Field Crops Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Fedeli, R., Fiaschi, T., Angiolini, C., Maccherini, S., Loppi, S., and Fanfarillo, E. (2023). Dose-Dependent and Species-Specific Effects of Wood Distillate Addition on the Germination Performance of Threatened Arable Plants. Plants, 12.","DOI":"10.3390\/plants12173028"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"169","DOI":"10.2134\/agronj1991.00021962008300010039x","article-title":"Interaction of salinity and temperature on the germination of crambe","volume":"83","author":"Fowler","year":"1991","journal-title":"Agron. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1007\/s10811-013-0078-4","article-title":"Effect of liquid seaweed extracts on growth of tomato seedlings (Solanum lycopersicum L.)","volume":"26","author":"Norrie","year":"2014","journal-title":"J. Appl. Phycol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e02245","DOI":"10.7554\/eLife.02245","article-title":"Hidden shift of the ionome of plants exposed to elevated CO2 depletes minerals at the base of human nutrition","volume":"3","author":"Loladze","year":"2014","journal-title":"eLife"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the False Discovery Rate: A Practical and Powerful Approach to MultipleTesting","volume":"57","author":"Benjamini","year":"1995","journal-title":"J. R. Stat. Soc. Ser. B Stat. Methodol."},{"key":"ref_36","unstructured":"R Core Team (2023). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_37","first-page":"413","article-title":"Response of sunflower to various pre-germination techniques for breaking seed dormancy","volume":"47","author":"Nasreen","year":"2015","journal-title":"Pak. J. Bot."},{"key":"ref_38","first-page":"450","article-title":"The subcellular basis of seed priming","volume":"99","author":"Varier","year":"2010","journal-title":"Curr. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1104\/pp.122.2.327","article-title":"Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not","volume":"122","author":"Groot","year":"2000","journal-title":"Plant Physiol."},{"key":"ref_40","first-page":"1127","article-title":"Effect of seed priming with ascorbic acid, salicylic acid and hydrogen peroxide on emergence, vigor and antioxidant activities of maize","volume":"11","author":"Ahmad","year":"2012","journal-title":"Afr. J. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/s13593-017-0466-2","article-title":"Wood vinegar seed priming improves yield and suppresses weeds in dryland direct-seeding rice under rainfed production","volume":"37","author":"Simma","year":"2017","journal-title":"Agron. Sustain. Dev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"155","DOI":"10.17660\/ActaHortic.2009.813.20","article-title":"Karrikinolide\u2014A phytoreactive compound derived from smoke with applications in horticulture, ecological restoration and agriculture","volume":"813","author":"Dixon","year":"2009","journal-title":"Acta Hortic."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.plantsci.2009.06.007","article-title":"Karrikins: A new family of plant growth regulators in smoke","volume":"177","author":"Chiwocha","year":"2009","journal-title":"Plant Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.scienta.2010.02.005","article-title":"Effect of smoke-water and a smoke-isolated butenolide on the growth and genotoxicity of commercial onion","volume":"124","author":"Kulkarni","year":"2010","journal-title":"Sci. Hortic."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1146\/annurev-arplant-042811-105545","article-title":"Regulation of seed germination and seedling growth by chemical signals from burning vegetation","volume":"63","author":"Nelson","year":"2012","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1006\/anbo.1996.0364","article-title":"The Effects of Ethanol on the Germination of Seeds of Japonica and Indica Rice (Oryza sativa L.) under Anaerobic and Aerobic Conditions","volume":"79","author":"Miyoshi","year":"1997","journal-title":"Ann. Bot."},{"key":"ref_47","first-page":"6","article-title":"Effect of different ethanol concentrations on seed germination of three turfgrass genera","volume":"2","author":"Salehi","year":"2008","journal-title":"Adv. Nat. Appl. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Chu, L., Liu, H., Zhang, Z., Zhan, Y., Wang, K., Yang, D., and Yu, J. (2022). Evaluation of Wood Vinegar as an Herbicide for Weed Control. Agronomy, 12.","DOI":"10.3390\/agronomy12123120"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/j.1439-037X.2006.00213.x","article-title":"Stimulation of rice (Oryza sativa L.) seedling vigour by smoke-water and butenolide","volume":"192","author":"Kulkarni","year":"2006","journal-title":"J. Agron. Crop Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s11258-023-01296-3","article-title":"Impacts of moderateness and cobalancedness in seed mass and coat thickness on seed dispersal effectiveness of Quercus variabilis by rodents","volume":"224","author":"Yang","year":"2023","journal-title":"Plant Ecol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/s11284-008-0524-y","article-title":"Effects of the pericarp on imbibition, seed germination, and seedling establishment in seeds of Hedysarum scoparium Fisch. et Mey","volume":"24","author":"Hu","year":"2008","journal-title":"Ecol. Res."}],"container-title":["Applied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3417\/14\/2\/631\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:44:47Z","timestamp":1760103887000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3417\/14\/2\/631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,11]]},"references-count":51,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,1]]}},"alternative-id":["app14020631"],"URL":"https:\/\/doi.org\/10.3390\/app14020631","relation":{},"ISSN":["2076-3417"],"issn-type":[{"value":"2076-3417","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,11]]}}}