{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T06:38:38Z","timestamp":1771915118848,"version":"3.50.1"},"reference-count":84,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T00:00:00Z","timestamp":1707523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund (ERDF)","award":["CENTRO-01-0145-FEDER-000012- HealthyAging2020"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000012- HealthyAging2020"]}]},{"name":"European Regional Development Fund (ERDF)","award":["CENTRO-01-0145-FEDER-022095"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-022095"]}]},{"name":"European Regional Development Fund (ERDF)","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}]},{"name":"European Regional Development Fund (ERDF)","award":["UIDP\/04539\/2020"],"award-info":[{"award-number":["UIDP\/04539\/2020"]}]},{"name":"European Regional Development Fund (ERDF)","award":["LA\/P\/0058\/2020"],"award-info":[{"award-number":["LA\/P\/0058\/2020"]}]},{"name":"European Regional Development Fund (ERDF)","award":["10.54499\/DL57\/2016\/CP1448\/CT0025"],"award-info":[{"award-number":["10.54499\/DL57\/2016\/CP1448\/CT0025"]}]},{"name":"COMPETE 2020\u2013Operational Programme for Competitiveness and Internationalisation","award":["CENTRO-01-0145-FEDER-000012- HealthyAging2020"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000012- HealthyAging2020"]}]},{"name":"COMPETE 2020\u2013Operational Programme for Competitiveness and Internationalisation","award":["CENTRO-01-0145-FEDER-022095"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-022095"]}]},{"name":"COMPETE 2020\u2013Operational Programme for Competitiveness and Internationalisation","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}]},{"name":"COMPETE 2020\u2013Operational Programme for Competitiveness and Internationalisation","award":["UIDP\/04539\/2020"],"award-info":[{"award-number":["UIDP\/04539\/2020"]}]},{"name":"COMPETE 2020\u2013Operational Programme for Competitiveness and Internationalisation","award":["LA\/P\/0058\/2020"],"award-info":[{"award-number":["LA\/P\/0058\/2020"]}]},{"name":"COMPETE 2020\u2013Operational Programme for Competitiveness and Internationalisation","award":["10.54499\/DL57\/2016\/CP1448\/CT0025"],"award-info":[{"award-number":["10.54499\/DL57\/2016\/CP1448\/CT0025"]}]},{"name":"Chantal Fernandes","award":["CENTRO-01-0145-FEDER-000012- HealthyAging2020"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000012- HealthyAging2020"]}]},{"name":"Chantal Fernandes","award":["CENTRO-01-0145-FEDER-022095"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-022095"]}]},{"name":"Chantal Fernandes","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}]},{"name":"Chantal Fernandes","award":["UIDP\/04539\/2020"],"award-info":[{"award-number":["UIDP\/04539\/2020"]}]},{"name":"Chantal Fernandes","award":["LA\/P\/0058\/2020"],"award-info":[{"award-number":["LA\/P\/0058\/2020"]}]},{"name":"Chantal Fernandes","award":["10.54499\/DL57\/2016\/CP1448\/CT0025"],"award-info":[{"award-number":["10.54499\/DL57\/2016\/CP1448\/CT0025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>Seaweeds have been explored as a natural resource of compounds of interest due to their bioactivities. Although many studies report the interest and the application of seaweeds in various areas, from food or human health to the economy, these data mostly focus on raw extracts and not on specific compounds, such as seaweed pigments. Fungal infections of the skin, nails, and hair caused by dermatophytes are the most common fungal infections worldwide. These pathologies require long periods of topical and\/or systemic treatment associated with adverse effects and increased antifungal resistance. So, this study had two objectives: the first was to isolate and characterize the pigments of the seaweeds Calliblepharis jubata and Fucus vesiculosus; the second was to assess their antifungal activity. The extraction of pigments was performed using a method of extraction by exhaustion, and the purification was achieved via column chromatography. Three techniques were used to characterize the pigments: thin-layer chromatography (TLC), UV\u2013visible spectrophotometry, and Fourier-transform infrared spectroscopy (FTIR). The antifungal activity against the three most common dermatophytes, Trichophyton rubrum, Trichophyton mentagrophytes, and Microsporum canis, was evaluated using a microdilution methodology, following the EUCAST international standards. It was possible to observe that the extracts obtained from the seaweed C. jubata, corresponding to the purified pigment phycobiliprotein, and the crude extract (an enriched extract) showed antifungal activity against the three fungal agents of human skin infection.<\/jats:p>","DOI":"10.3390\/app14041456","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T03:50:27Z","timestamp":1707709827000},"page":"1456","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Seaweed Calliblepharis jubata and Fucus vesiculosus Pigments: Anti-Dermatophytic Activity"],"prefix":"10.3390","volume":"14","author":[{"given":"Louisa","family":"Gomes","sequence":"first","affiliation":[{"name":"Marine Resources, Conservation and Technology, Marine Algae Lab, CFE\u2014Centre for Functional Ecology, Science for People & Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5244-221X","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Cotas","sequence":"additional","affiliation":[{"name":"Marine Resources, Conservation and Technology, Marine Algae Lab, CFE\u2014Centre for Functional Ecology, Science for People & Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7865-9946","authenticated-orcid":false,"given":"Chantal","family":"Fernandes","sequence":"additional","affiliation":[{"name":"CNC\u2014Center for Neurosciences and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9347-0535","authenticated-orcid":false,"given":"Teresa","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"CNC\u2014Center for Neurosciences and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal"},{"name":"FMUC\u2014Faculty of Medicine, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6819-0619","authenticated-orcid":false,"given":"Leonel","family":"Pereira","sequence":"additional","affiliation":[{"name":"Marine Resources, Conservation and Technology, Marine Algae Lab, CFE\u2014Centre for Functional Ecology, Science for People & Planet, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"739","DOI":"10.3390\/md9050739","article-title":"Anti-Phytopathogenic Activities of Macro-Algae Extracts","volume":"9","author":"Dorta","year":"2011","journal-title":"Mar. 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