{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T01:08:36Z","timestamp":1776128916253,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T00:00:00Z","timestamp":1551830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Applied Sciences"],"abstract":"<jats:p>(1) Background: Multiple attempts have been conducted to correlate milk keeping quality with chemical, physical or bacteriological parameters. These methods only measure the chemical changes in milk produced by bacteria. Headspace solid-phase micro-extraction (HS-SPME) is an economic and recent method used to measure both volatile compounds and microbial load in milk, also allowing to keep the quality of the milk product. (2) Methods: The present study was conducted to identify and measure the off-flavoring volatile compounds through gas chromatography coupled with flame ionization detector (GC-FID) and the microbial load of pasteurized fluid milk stored at different temperatures, as a possible indicator of its keeping quality. (3) Results: The highest results were obtained to acetone, followed by butanone, pentanal and ethanol. These mean values were significantly enhanced from the 0 to 19th day of storage, at 10 \u00b0C. At day 19th, the minimum score for aroma, flavor and overall acceptability were also recorded as 4.33 \u00b1 0.17, 4.02 \u00b1 0.06, 4.00 \u00b1 0.04, respectively. Likewise, maximum values for standard plate count (Log10 CFU 15.54 \u00b1 0.40 mL\u22121) and total psychotroph count (Log10 CFU 11.67 \u00b1 0.30mL\u22121) were reported at 10 \u00b0C and 4 \u00b0C. (4) Conclusion: HS-SPME\/GC-FID methodology revealed to be very sensitive and capable to be applied in volatile compounds quantification in pasteurized milk produced during the storage period at different temperatures.<\/jats:p>","DOI":"10.3390\/app9050959","type":"journal-article","created":{"date-parts":[[2019,3,7]],"date-time":"2019-03-07T10:52:22Z","timestamp":1551955942000},"page":"959","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Measurement of Off-Flavoring Volatile Compounds and Microbial Load as a Probable Marker for Keeping Quality of Pasteurized Milk"],"prefix":"10.3390","volume":"9","author":[{"given":"Anjum","family":"Rashid","sequence":"first","affiliation":[{"name":"School of Food Sciences, Washington State University, Pullman, WA 99164-5110, USA"}]},{"given":"Imran","family":"Javed","sequence":"additional","affiliation":[{"name":"Department of Dairy Technology, University of Veterinary and Animal Sciences, Lahore 5400, Pakistan"}]},{"given":"Barbara","family":"Rasco","sequence":"additional","affiliation":[{"name":"School of Food Sciences, Washington State University, Pullman, WA 99164-5110, USA"}]},{"given":"Shyam","family":"Sablani","sequence":"additional","affiliation":[{"name":"School of Food Sciences, Washington State University, Pullman, WA 99164-5110, USA"}]},{"given":"Muhammad","family":"Ayaz","sequence":"additional","affiliation":[{"name":"Department of Dairy Technology, University of Veterinary and Animal Sciences, Lahore 5400, Pakistan"}]},{"given":"Muhammad","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, University of Veterinary and Animal Sciences, Lahore 5400, Pakistan"}]},{"given":"Muhammad","family":"Abdullah","sequence":"additional","affiliation":[{"name":"Department of Dairy Technology, University of Veterinary and Animal Sciences, Lahore 5400, Pakistan"}]},{"given":"Muhammad","family":"Imran","sequence":"additional","affiliation":[{"name":"University Institute of Diet and Nutritional Sciences, The University of Lahore, Lahore 54000, Pakistan"}]},{"given":"Tanweer","family":"Gondal","sequence":"additional","affiliation":[{"name":"School of Exercise and Nutrition, Deakin University, Victoria 3217, Australia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7338-6237","authenticated-orcid":false,"given":"Muhammad","family":"Afzal","sequence":"additional","affiliation":[{"name":"Department of Biosciences, COMSATS University Islamabad, Tarlai Kalan, Islamabad 46000, Pakistan"}]},{"given":"Muhammad","family":"Atif","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72341, Saudi Arabia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6900-9797","authenticated-orcid":false,"given":"Bahare","family":"Salehi","sequence":"additional","affiliation":[{"name":"Student Research Committee, Bam University of Medical Sciences, Bam 44340847, Iran"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8633-2230","authenticated-orcid":false,"given":"C\u00e9lia","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s\/n, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7301-8151","authenticated-orcid":false,"given":"Javad","family":"Sharifi-Rad","sequence":"additional","affiliation":[{"name":"Food Safety Research Center (salt), Semnan University of Medical Sciences, Semnan 3519899951, Iran"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5934-5201","authenticated-orcid":false,"given":"Nat\u00e1lia","family":"Martins","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Porto, Alameda Prof. Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"},{"name":"Institute for Research and Innovation in Health (i3S), University of Porto, 4200-135 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E15","DOI":"10.3168\/jds.S0022-0302(06)72360-8","article-title":"Influence of raw milk quality on fluid milk shelf life","volume":"89","author":"Barbano","year":"2006","journal-title":"J. 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