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Nano"],"abstract":"<jats:p>Bacterial spot (BS) of tomato (S. lycopersicum), caused by Xanthomonas spp., namely X. euvesicatoria (Xeu), is one of the major threats for the production of this crop worldwide. Developing new biocontrol solutions against this disease will allow disease management strategies to be less based on Cu compounds. Nanoclays, such as montmorillonite (NMT), have been under investigation for their antimicrobial activity, or as delivery tools\/stabilizers for organic compounds, such as essential oils (EOs), that also possess antimicrobial activity against plant pathogens. This work aims to assess how the application of NMT alone or incorporating S. montana EO on Xeu-infected hosts (var. Oxheart) affects the shoots\u2019 redox status and antioxidant defense mechanisms. In vitro shoots, grown on Murashige and Skoog medium, were divided in two groups, Xeu-infected and uninfected (control) shoots. Shoots of each group were then treated with NMT, S. montana EO, EO-NMT. Results show that the NMT was able to reduce Xeu bacterial amount, while reducing ROS production and keeping the transcript levels of the defense-related genes close to those of the control. When applied to uninfected shoots, the treatments triggered the production of ROS and upregulated the phenylpropanoid and hormone pathway, which suggest that they act as defense elicitors. Globally, the results indicate that NMT has the potential to integrate BS management strategies, due to its antimicrobial activity, and that EO and\/or nanoclays could be successfully employed as new disease preventive strategies, since they enhance the healthy shoots\u2019 defense, thus potentially limiting the pathogen establishment.<\/jats:p>","DOI":"10.3390\/applnano3030009","type":"journal-article","created":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T11:15:05Z","timestamp":1656933305000},"page":"126-142","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Montmorillonite Nanoclay and Formulation with Satureja montana Essential Oil as a Tool to Alleviate Xanthomonas euvesicatoria Load on Solanum lycopersicum"],"prefix":"10.3390","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9080-2469","authenticated-orcid":false,"given":"Paulo R.","family":"Oliveira-Pinto","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1695-2762","authenticated-orcid":false,"given":"Nuno","family":"Mariz-Ponte","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, Portugal"}]},{"given":"Renato L.","family":"Gil","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5603-0140","authenticated-orcid":false,"given":"Edite","family":"Cunha","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3422-3013","authenticated-orcid":false,"given":"C\u00e9lia G.","family":"Amorim","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1523-5043","authenticated-orcid":false,"given":"Maria C. B. S. M.","family":"Montenegro","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"LAQV-REQUIMTE, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal"}]},{"given":"Manuel","family":"Fernandes-Ferreira","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, Portugal"},{"name":"GreenUPorto & Inov4Agro, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, Portugal"},{"name":"CITAB & Inov4Agro, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8979-1229","authenticated-orcid":false,"given":"Rose M. O. 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