{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T17:39:44Z","timestamp":1772559584913,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad Nacional Jorge Basadre Grohmann through the program \u201cFondos del canon, sobrecanon y regal\u00edas mineras\u201d","award":["11174-2023-UNJBG"],"award-info":[{"award-number":["11174-2023-UNJBG"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT) for AGS","award":["2023.07625.CEECIND\/CP2848\/CT0010"],"award-info":[{"award-number":["2023.07625.CEECIND\/CP2848\/CT0010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aquaculture Journal"],"abstract":"<jats:p>Background: The reproductive cycle of Octopus mimus is regulated by environmental and hormonal factors, with photoperiod playing a key role in spawning induction and reproductive maturation. Understanding its underlying molecular mechanisms is essential for developing strategies to enhance controlled reproduction in aquaculture. Methods: We analyzed the expression of genes involved in the photoperiod-activated spawning induction cascade in the optic lobe and its downstream effects on the oviducal gland by performing transcriptomic analyses on females exposed to continuous light (24:0), which inhibits reproductive development, and a natural photoperiod, which induces spawning. The mRNA sequencing (RNA-Seq), quality control, gene annotation, and differential expression analyses were conducted using edgeR. Results: Spawning was completely inhibited under constant light, while 80% of control females spawned. Expression profiling revealed 89 downregulated and 34 upregulated genes in the optic lobe, and 178 downregulated and 237 upregulated genes in the oviducal gland (FDR &lt; 0.05, |log2FC| \u2265 2), including key orthologs such as FMRFamide and myomodulin. Conclusions: These results show that the optic lobe integrates photoperiodic cues that modulate reproductive activation via a neuroendocrine cascade and coordinates spawning regulation through the oviducal gland, providing insights for improving reproductive control in aquaculture systems.<\/jats:p>","DOI":"10.3390\/aquacj6010007","type":"journal-article","created":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T15:22:21Z","timestamp":1772551341000},"page":"7","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Photoperiod-Mediated Transcriptomic Regulation of Spawning in Octopus mimus"],"prefix":"10.3390","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0940-2594","authenticated-orcid":false,"given":"Calixto","family":"Quispe-Pilco","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Agropecuarias, Escuela Profesional de Ingenier\u00eda Pesquera, Universidad Nacional Jorge Basadre Grohmann, Av. Miraflores s\/n, Tacna 23001, Peru"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3356-2524","authenticated-orcid":false,"given":"In\u00eas","family":"Ferreira","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5803-185X","authenticated-orcid":false,"given":"Diogo","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"}]},{"given":"Rui","family":"Resende-Pinto","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9973-4861","authenticated-orcid":false,"given":"Andr\u00e9 Gomes","family":"dos Santos","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7227-1465","authenticated-orcid":false,"given":"Freddy Walter","family":"Delgado-Cabrera","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Agropecuarias, Escuela Profesional de Ingenier\u00eda Pesquera, Universidad Nacional Jorge Basadre Grohmann, Av. Miraflores s\/n, Tacna 23001, Peru"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8871-3587","authenticated-orcid":false,"given":"Khiara Aliyah Bet","family":"Moreno-Salazar-Calderon","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Agropecuarias, Escuela Profesional de Ingenier\u00eda Pesquera, Universidad Nacional Jorge Basadre Grohmann, Av. Miraflores s\/n, Tacna 23001, Peru"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3208-8062","authenticated-orcid":false,"given":"Cintia P.","family":"Fern\u00e1ndez-C\u00e1rdenas","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Agropecuarias, Escuela Profesional de Ingenier\u00eda Pesquera, Universidad Nacional Jorge Basadre Grohmann, Av. Miraflores s\/n, Tacna 23001, Peru"}]},{"given":"Fredy Esfrayn","family":"Tapia-Alave","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Agropecuarias, Escuela Profesional de Ingenier\u00eda Pesquera, Universidad Nacional Jorge Basadre Grohmann, Av. Miraflores s\/n, Tacna 23001, Peru"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7697-386X","authenticated-orcid":false,"given":"L. Filipe C.","family":"Castro","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7733-1903","authenticated-orcid":false,"given":"Manuel","family":"Nande","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,3]]},"reference":[{"key":"ref_1","first-page":"75","article-title":"Influencia de la luz en la maduraci\u00f3n sexual de hembras Octopus mimus","volume":"14","author":"Olivares","year":"1995","journal-title":"Estud. Ocean."},{"key":"ref_2","unstructured":"Jim\u00e9nez, J., Louis, V., Yamashiro, C., and Kochzius, M. (2019). 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