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This study intends to assess the deposition rates of total settleable dust and bioburden in the indoor air of dwellings onto quartz fiber filters and electrostatic dust collectors (EDCs), respectively. EDC extracts were inoculated onto malt extract agar (MEA) and dichloran glycerol (DG18) agar-based media used for fungal contamination characterization, while tryptic soy agar (TSA) was applied for total bacteria assessment, and violet red bile agar (VRBA) for Gram-negative bacteria. Azole-resistance screening and molecular detection by qPCR was also performed. Dust loading rates ranged from 0.111 to 3.52, averaging 0.675 \u03bcg cm\u22122 day\u22121. Bacterial counts ranged from undetectable to 16.3 colony-forming units (CFU) m\u22122 day\u22121 and to 2.95 CFU m\u22122 day\u22121 in TSA and VRBA, respectively. Fungal contamination ranged from 1.97 to 35.4 CFU m\u22122 day\u22121 in MEA, and from undetectable to 48.8 CFU m\u22122 day\u22121 in DG18. Penicillium sp. presented the highest prevalence in MEA media (36.2%) and Cladosporium sp. in DG18 (39.2%). It was possible to observe: (a) settleable dust loadings and fungal contamination higher in dwellings with pets; (b) fungal species considered indicators of harmful fungal contamination; (c) Aspergillus section Candidi identified in supplemented media with voriconazole and posaconazole; (d) specific housing typologies and (e) specific housing characteristics influencing the microbial contamination.<\/jats:p>","DOI":"10.3390\/atmos12030378","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T22:13:10Z","timestamp":1615759990000},"page":"378","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Loading Rates of Dust and Bioburden in Dwellings in an Inland City of Southern Europe"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1545-6479","authenticated-orcid":false,"given":"Carla","family":"Viegas","sequence":"first","affiliation":[{"name":"H&amp;TRC\u2014Health &amp; Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"},{"name":"NOVA National School of Public Health, Public Health Research Centre, Univer-Sidade NOVA de Lisboa, 1990-096 Lisbon, Portugal"},{"name":"Comprehensive Health Research Center (CHRC), 1169-056 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2704-6995","authenticated-orcid":false,"given":"Marta","family":"Dias","sequence":"additional","affiliation":[{"name":"H&amp;TRC\u2014Health &amp; Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"}]},{"given":"Beatriz","family":"Almeida","sequence":"additional","affiliation":[{"name":"H&amp;TRC\u2014Health &amp; Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"}]},{"given":"Estela","family":"Vicente","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Environment and Planning, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Carla","family":"Candeias","sequence":"additional","affiliation":[{"name":"Geobiosciences, Geotechnologies and Geoengineering Research Centre (GeoBioTec), Department of Geosciences, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1496-2609","authenticated-orcid":false,"given":"Liliana","family":"Aranha Caetano","sequence":"additional","affiliation":[{"name":"H&amp;TRC\u2014Health &amp; Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"},{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, 1990-096 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4165-7052","authenticated-orcid":false,"given":"Elisabete","family":"Carolino","sequence":"additional","affiliation":[{"name":"H&amp;TRC\u2014Health &amp; Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3231-3186","authenticated-orcid":false,"given":"C\u00e9lia","family":"Alves","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Environment and Planning, University of Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1002\/clen.200800220","article-title":"Bacteria and Fungi Levels in Various Indoor and Outdoor Environments in Ankara, Turkey","volume":"37","author":"Mentese","year":"2009","journal-title":"CLEAN Soil Air Water"},{"key":"ref_2","first-page":"172","article-title":"Indoor air mycoflora of residential dwellings in Jos metropolis","volume":"10","author":"Ayanbimpe","year":"2010","journal-title":"Afr. 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