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At the forefront of this revolutionary progress are stem cells. Stem cells typically reside in specialized environments in vivo, known as microenvironments or niches, which play critical roles in regulating stem cell behavior and determining their fate. Therefore, understanding the complex microenvironments that surround stem cells is crucial for advancing treatment options in regenerative medicine and tissue engineering applications. Several research articles have made significant contributions to this field by exploring the interactions between stem cells and their surrounding niches, investigating the influence of biomechanical and biochemical cues, and developing innovative strategies for tissue regeneration. This review highlights the key findings and contributions of these studies, shedding light on the diverse applications that may arise from the understanding of stem cell microenvironments, thus harnessing the power of these microenvironments to transform the landscape of medicine and offer new avenues for regenerative therapies.<\/jats:p>","DOI":"10.3390\/bioengineering11030289","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T05:04:12Z","timestamp":1710911052000},"page":"289","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Unlocking the Potential of Stem Cell Microenvironments In Vitro"],"prefix":"10.3390","volume":"11","author":[{"given":"Chiara","family":"Scodellaro","sequence":"first","affiliation":[{"name":"Department of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"}]},{"given":"Raquel R.","family":"Pina","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5177-6237","authenticated-orcid":false,"given":"Frederico Castelo","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2079-2864","authenticated-orcid":false,"given":"Paola","family":"Sanjuan-Alberte","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4651-2832","authenticated-orcid":false,"given":"Tiago G.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s13287-019-1165-5","article-title":"Stem Cells: Past, Present, and Future","volume":"10","author":"Zakrzewski","year":"2019","journal-title":"Stem Cell Res. 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