{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T12:50:26Z","timestamp":1763988626331,"version":"3.45.0"},"reference-count":68,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>In this study, two native microalgae, Chlorella sp. MC18 (CH) and Scenedesmus sp. MJ23-R (SC) were cultivated in bubble column photobioreactors for wastewater treatment. Domestic wastewater (DWW) was used as the main culture medium, alone (100%) and blended (10%) with vinasse, whey, or agro-food waste (AFW), respectively. Both species thrived in 100% DWW, achieving significantly high removal efficiencies for chemical oxygen demand, total nitrogen, and total phosphorus. Mineral removal exceeded 90% in all blended systems, highlighting the strong nutrient uptake capacity of both strains. The maximum specific growth rate (\u00b5max) in 100% DWW was higher for SC than in standard BG11 medium, and supplementation with vinasse, whey, or AFW further increased \u00b5max for both species. Blending DWW significantly enhanced microalgal biomass and lipid production compared to 100% DWW. Lipid production (max., 374 mg L\u22121), proximate lipid composition (max., 30.4%), and lipid productivity (max., 52.9 mg L\u22121 d\u22121) significantly increased in all supplemented cultures relative to DWW alone, demonstrating the potential of co-substrate supplementation to optimize microalgal cultivation. This study contributes to reducing the water footprint and fills a gap in the bioprocessing potential of algae-based systems, highlighting wastewater blending as a circular economy-aligned approach that supports sustainable bioprocesses and resource recovery.<\/jats:p>","DOI":"10.3390\/bioengineering12121291","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T11:47:22Z","timestamp":1763984842000},"page":"1291","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sustainable Nutrient Recovery from Wastewater Mixture to Optimize Microalgal Lipid Production: A Vision of Zero Water Footprint"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0694-8727","authenticated-orcid":false,"given":"Marco Alberto","family":"Mamani Condori","sequence":"first","affiliation":[{"name":"Facultad de Ingenier\u00eda de Procesos, Universidad Nacional de San Agust\u00edn de Arequipa, Arequipa 04000, Peru"}]},{"given":"Danae","family":"Colque Ollachica","sequence":"additional","affiliation":[{"name":"Facultad de Ingenier\u00eda de Procesos, Universidad Nacional de San Agust\u00edn de Arequipa, Arequipa 04000, Peru"}]},{"given":"Abel Roberto","family":"Ccapa Loncone","sequence":"additional","affiliation":[{"name":"Facultad de Ingenier\u00eda de Procesos, Universidad Nacional de San Agust\u00edn de Arequipa, Arequipa 04000, Peru"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2738-5102","authenticated-orcid":false,"given":"Jos\u00e9 C. M.","family":"Pires","sequence":"additional","affiliation":[{"name":"LEPABE, ALiCE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6402-2389","authenticated-orcid":false,"given":"Ana Mar\u00eda","family":"Gagneten","sequence":"additional","affiliation":[{"name":"Laboratorio de Ecotoxicolog\u00eda, Facultad de Humanidades y Ciencias, Universidad Nacional del Litoral, RN 168, Km 0, Santa Fe S3000ADQ, Argentina"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.5194\/essd-13-237-2021","article-title":"Country-level and gridded estimates of wastewater production, collection, treatment and reuse","volume":"13","author":"Jones","year":"2021","journal-title":"Earth Syst. Sci. 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