{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T21:38:43Z","timestamp":1774647523767,"version":"3.50.1"},"reference-count":77,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T00:00:00Z","timestamp":1649894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portugal 2020 - Algarve Regional Operational Programme","award":["ALG-01-0247-FEDER-069971 \u2013 ALLARVAE"],"award-info":[{"award-number":["ALG-01-0247-FEDER-069971 \u2013 ALLARVAE"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>S. costatum and C. calcitrans are two cosmopolitan high-value centric diatoms, with a rich nutritional profile. The following work optimised the culture medium of S. costatum and C. calcitrans cultures, respectively, in a stepwise process as follows: 2.4 mM and 1.2 mM of silicate, 4 mM of nitrate, 100 \u00b5M of phosphate, 20 and 80 \u00b5M iron, and 0.5 mL L\u22121 of micronutrients. The results that were obtained revealed an increase in biomass productivity with a 1.8- and 3.2-fold increase in biomass that was produced by S. costatum and C. calcitrans, respectively. The biochemical profile showed an increase in high-value PUFAs such as 2.6-fold and 2.3-fold increase in EPA for S. costatum and C. calcitrans, respectively, whilst a 2.6-fold increase in DHA was detected in S. costatum cultures. The present work provides the basic tools for the industrial cultivation of S. costatum and C. calcitrans with enhanced productivity as well as improved biomass quality, two factors which are highly relevant for a more effective application of these diatoms to aquaculture and nutraceutical production.<\/jats:p>","DOI":"10.3390\/biology11040594","type":"journal-article","created":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T21:44:06Z","timestamp":1649972646000},"page":"594","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Optimisation of Biomass Production and Nutritional Value of Two Marine Diatoms (Bacillariophyceae), Skeletonema costatum and Chaetoceros calcitrans"],"prefix":"10.3390","volume":"11","author":[{"given":"Carolina R. V.","family":"Bastos","sequence":"first","affiliation":[{"name":"Centre of Marine Sciences, Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal"},{"name":"Necton S.A., Belamandil, 8700-152 Olh\u00e3o, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7509-2216","authenticated-orcid":false,"given":"In\u00eas B.","family":"Maia","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal"},{"name":"Necton S.A., Belamandil, 8700-152 Olh\u00e3o, Portugal"}]},{"given":"Hugo","family":"Pereira","sequence":"additional","affiliation":[{"name":"GreenCoLab-Associa\u00e7\u00e3o Oceano Verde, Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal"}]},{"given":"Jo\u00e3o","family":"Navalho","sequence":"additional","affiliation":[{"name":"Necton S.A., Belamandil, 8700-152 Olh\u00e3o, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3101-693X","authenticated-orcid":false,"given":"Jo\u00e3o C. S.","family":"Varela","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal"},{"name":"GreenCoLab-Associa\u00e7\u00e3o Oceano Verde, Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.biortech.2018.08.116","article-title":"Optimizing Culture Conditions for Heterotrophic-Assisted Photoautotrophic Biofilm Growth of Chlorella vulgaris to Simultaneously Improve Microalgae Biomass and Lipid Productivity","volume":"270","author":"Ye","year":"2018","journal-title":"Bioresour. 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