{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:24:01Z","timestamp":1762341841589,"version":"build-2065373602"},"reference-count":70,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T00:00:00Z","timestamp":1652400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["BD\/26033\/2005","UIDB\/04551\/2020"],"award-info":[{"award-number":["BD\/26033\/2005","UIDB\/04551\/2020"]}]},{"DOI":"10.13039\/501100001871","name":"R&amp;D Unit","doi-asserted-by":"publisher","award":["BD\/26033\/2005","UIDB\/04551\/2020"],"award-info":[{"award-number":["BD\/26033\/2005","UIDB\/04551\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria, I.P.","award":["BD\/26033\/2005","UIDB\/04551\/2020"],"award-info":[{"award-number":["BD\/26033\/2005","UIDB\/04551\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Platypus cylindrus is the most common ambrosia beetle in stands of Quercus suber in Portugal. This insect farms specialized fungi in sapwood galleries, using its mycangia to carry and store these organisms. Some ectosymbiotic fungi carried by P. cylindrus are phytopathogenic and cause extensive tree mortality and severe economic losses. To understand the role of P. cylindrus fungal symbionts in stands of Q. suber we examined beetle galleries present in declining and\/or dying cork oak trees during field surveys. Logs with active galleries were obtained in situ and from captured emerging beetles. Insects were aseptically dissected, and their mycangia and intestine were retrieved. Morphological and molecular profiles of fungal isolates obtained from cultured insect parts were carried out to accurately characterize and identify isolated fungi.\u00a0Molecular characterizations were performed with DNA sequence data from four loci, i.e., LSU, SSU, 5.8S-ITS2-28S, and TUB. Morphological results consistently showed a collection of Ophiostoma-like fungal axenic isolates, while phylogenies inferred that this collection constitutes an undescribed taxon reported herein for the first time in association with P. cylindrus in Portuguese cork oak stands. The novel species was erected as Ceratocystiopsis quercina sp. nov. and constitutes a new phytopathogenic fungal species associated with symptoms of vegetative cork oak decline.<\/jats:p>","DOI":"10.3390\/biology11050750","type":"journal-article","created":{"date-parts":[[2022,5,14]],"date-time":"2022-05-14T09:27:56Z","timestamp":1652520476000},"page":"750","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Ceratocystiopsis quercina sp. nov. Associated with Platypus cylindrus on Declining Quercus suber in Portugal"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6883-5288","authenticated-orcid":false,"given":"Maria L.","family":"In\u00e1cio","sequence":"first","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta do Marqu\u00eas, 2780-159 Oeiras, Portugal"},{"name":"GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa (ITQN NOVA), Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"given":"Jos\u00e9","family":"Marcelino","sequence":"additional","affiliation":[{"name":"Entomology and Nematology Department, University of Florida, Gainesville, FL 32608, USA"}]},{"given":"Arlindo","family":"Lima","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Environment Agriculture and Food, Instituto Superior de Agronomia (ISA), University of Lisbon, Tapada de Ajuda, 1349-017 Lisbon, Portugal"},{"name":"LPVVA\u2014Laborat\u00f3rio de Patologia Vegetal \u201cVer\u00edssimo de Almeida\u201d, Instituto Superior de Agronomia (ISA), University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6297-2938","authenticated-orcid":false,"given":"Edmundo","family":"Sousa","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta do Marqu\u00eas, 2780-159 Oeiras, Portugal"},{"name":"GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa (ITQN NOVA), Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4318-1142","authenticated-orcid":false,"given":"Filomena","family":"N\u00f3brega","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta do Marqu\u00eas, 2780-159 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,13]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Contribui\u00e7\u00e3o para a hist\u00f3ria da Entomologia em portugal","volume":"6","author":"Seabra","year":"1939","journal-title":"Publica\u00e7\u00f5es Dir. 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