{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T18:33:41Z","timestamp":1773426821124,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T00:00:00Z","timestamp":1675814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["UIDB\/CVT\/00772\/2020"],"award-info":[{"award-number":["UIDB\/CVT\/00772\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["DL57\/2016\/CP1327\/CT0006"],"award-info":[{"award-number":["DL57\/2016\/CP1327\/CT0006"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["UIDB\/CVT\/00772\/2020"],"award-info":[{"award-number":["UIDB\/CVT\/00772\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese Foundation for Science and Technology (FCT)","doi-asserted-by":"publisher","award":["DL57\/2016\/CP1327\/CT0006"],"award-info":[{"award-number":["DL57\/2016\/CP1327\/CT0006"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Ovarian ageing stands as the major contributor towards fertility loss. As such, there is an urge for studies addressing the mechanisms that promote ovarian ageing and new strategies aiming to delay it. Recently, the presence of a unique population of multinucleated giant cells has been identified in the ovaries of reproductively aged mice. These cells have been considered hallmarks of ovarian ageing. However, up to date multinucleated giant cells have only been described in the ovaries of the mice. Therefore, the aim of the present work was to evaluate and characterize the presence of such hallmarks of ovarian ageing in the sheep and the goat. In this study, ovaries from juvenile (6 months) and mature animals (18\u201324 months) were used. The hematoxylin and eosin technique was performed to describe the ovarian morphology and evaluate the ovarian follicle reserve pool. Sudan black B staining and the detection of autofluorescence emission were used to identify and characterize the presence of multinucleated giant cells. Statistical analyses were performed with GraphPad Prism 9.0.0. A decrease in the follicle reserve pool and the presence of multinucleated giant cells, with lipofuscin accumulation and the emission of autofluorescence, were observed in the ovaries of the mature animals of both species. Our results support the interest in the use of the ovine and the caprine model, that share physiological and pathophysiological characteristics with humans, in future studies addressing ovarian ageing.<\/jats:p>","DOI":"10.3390\/biology12020270","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T04:57:41Z","timestamp":1675832261000},"page":"270","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Contribution of the Sheep and the Goat Model to the Study of Ovarian Ageing"],"prefix":"10.3390","volume":"12","author":[{"given":"Lu\u00eds","family":"Montenegro","sequence":"first","affiliation":[{"name":"Animal and Veterinary Research Center (CECAV), Universidade de Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Veterinary Hospital Refer\u00eancia Veterin\u00e1ria Montenegro, Rua da P\u00f3voa 34, 4000-395 Porto, Portugal"},{"name":"AL4AnimalS-Associate Laboratory for Animal and Veterinary Sciences, 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9879-8756","authenticated-orcid":false,"given":"Paulo","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Unidade de Biologia Experimental, Departamento de Biomedicina, Faculdade de Medicina, Universidade do Porto, Alameda Professor Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"},{"name":"Instituto de Biologia Molecular e Celular (IBMC), i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal"}]},{"given":"Adriana Costa","family":"Guerreiro","sequence":"additional","affiliation":[{"name":"Unidade de Biologia Experimental, Departamento de Biomedicina, Faculdade de Medicina, Universidade do Porto, Alameda Professor Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"},{"name":"Instituto de Biologia Molecular e Celular (IBMC), i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal"}]},{"given":"Catarina","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Unidade de Biologia Experimental, Departamento de Biomedicina, Faculdade de Medicina, Universidade do Porto, Alameda Professor Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"},{"name":"Instituto de Biologia Molecular e Celular (IBMC), i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal"}]},{"given":"Anabela","family":"Pinto","sequence":"additional","affiliation":[{"name":"Unidade de Biologia Experimental, Departamento de Biomedicina, Faculdade de Medicina, Universidade do Porto, Alameda Professor Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3036-3211","authenticated-orcid":false,"given":"Henrique","family":"Almeida","sequence":"additional","affiliation":[{"name":"Unidade de Biologia Experimental, Departamento de Biomedicina, Faculdade de Medicina, Universidade do Porto, Alameda Professor Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"},{"name":"Instituto de Biologia Molecular e Celular (IBMC), i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal"},{"name":"Ginecologia-Obstetr\u00edcia, Hospital CUF, Estrada da Circunvala\u00e7\u00e3o 14341, 4100-180 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9566-3100","authenticated-orcid":false,"given":"Ana","family":"Martins-Bessa","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Center (CECAV), Universidade de Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"AL4AnimalS-Associate Laboratory for Animal and Veterinary Sciences, 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6736-3469","authenticated-orcid":false,"given":"Elisabete","family":"Silva","sequence":"additional","affiliation":[{"name":"Unidade de Biologia Experimental, Departamento de Biomedicina, Faculdade de Medicina, Universidade do Porto, Alameda Professor Hern\u00e2ni Monteiro, 4200-319 Porto, Portugal"},{"name":"Instituto de Biologia Molecular e Celular (IBMC), i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal"},{"name":"Departamento de Imuno-Fisiologia e Farmacologia, Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar (ICBAS), Universidade do Porto, Rua de Jorge Viterbo Ferreira, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1093\/humupd\/dmr026","article-title":"Why do people postpone parenthood? 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